FlyBase Allele Report: Dmel\pont[5.1]

General Information

Symbol

Dmel\pont^5.1

Species

D. melanogaster

Name

FlyBase ID

FBal0120381

Feature type

allele

Associated gene

Dmel\pont

Associated Insertion(s)

Carried in Construct

Also Known As

Pontin^5.1

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Bauer et al., 2000)

Mutagen

P-element activity

(Bauer et al., 2000)

Progenitor genotype

pont^S022905a

(Bauer et al., 2000)

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | somatic clone

(Bellosta et al., 2005)

lethal - all die before end of larval stage | recessive

(Bellosta et al., 2005, Bauer et al., 2000)

some die during larval stage | recessive

(Bellosta et al., 2005, Bauer et al., 2000)

suppressor of variegation | dominant, with In(1)w^m4

(Prozzillo et al., 2021)

Phenotype Manifest In

wing | somatic clone

(Bellosta et al., 2005)

Detailed Description

Statement

Reference

Homozygous pont^5.1 larvae hatch at normal rates, showing that the zygotic product is not essential for embryogenesis. The mutant larvae live for up to 7 days but grow only minimally in size. Clones of pont^5.1 cells located within phenotypically wild-type wing imaginal discs grow poorly, indicating that the observed defects are cell-autonomous. Only a few small pont^5.1 clones survive at 72 hours after induction of the clones, with none observed after 120 hours, suggesting cell competition. In contrast, when the cells surrounding the pont^5.1 clones are are growth impaired, the mutant clones thrive even at 120 hours after induction. pont^5.1 cells are not smaller than wild-type control cells.

(Bellosta et al., 2005)

Mutant animals die at first instar larval stage.

(Bauer et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

brm², pont[+]/pont^5.1 has visible | dominant phenotype, suppressible by pont^UAS.cDa/Scer\GAL4^arm.PS

(Diop et al., 2008)

Enhancer of

Statement

Reference

pont[+]/pont^5.1 is an enhancer of visible | dominant phenotype of brm²

(Diop et al., 2008)

pont[+]/pont^5.1 is an enhancer of abnormal developmental rate phenotype of Myc^P0

(Bellosta et al., 2005)

pont[+]/pont^5.1 is an enhancer of partially lethal - majority die phenotype of Myc^P0

(Bellosta et al., 2005)

NOT Enhancer of

Statement

Reference

rept³⁵, rept[+], pont[+], pont^5.1 is a non-enhancer of visible | dominant phenotype of brm²

(Diop et al., 2008)

Other

Statement

Reference

Myc^P0, pont[+]/pont^5.1 has decreased cell number phenotype

(Bellosta et al., 2005)

Myc^P0, pont[+]/pont^5.1 has decreased cell size phenotype

(Bellosta et al., 2005)

pont^5.1, rept³⁵ has lethal | larval stage phenotype

(Bellosta et al., 2005)

Myc^P0, pont^5.1 has decreased cell number phenotype

(Bellosta et al., 2005)

Myc^P0, pont^5.1 has decreased cell size phenotype

(Bellosta et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

brm², pont[+]/pont^5.1 has wing phenotype, suppressible by pont^UAS.cDa/Scer\GAL4^arm.PS

(Diop et al., 2008)

Enhancer of

Statement

Reference

pont[+]/pont^5.1 is an enhancer of wing phenotype of brm²

(Diop et al., 2008)

pont[+]/pont^5.1 is an enhancer of eye phenotype of Myc^P0

(Bellosta et al., 2005)

pont[+]/pont^5.1 is an enhancer of ommatidium phenotype of Myc^P0

(Bellosta et al., 2005)

pont^5.1 is an enhancer of wing margin phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Bauer et al., 2000)

NOT Enhancer of

Statement

Reference

rept³⁵, rept[+], pont[+], pont^5.1 is a non-enhancer of wing phenotype of brm²

(Diop et al., 2008)

Suppressor of

Statement

Reference

pont^5.1 is a suppressor of wing margin bristle | ectopic phenotype of Scer\GAL4^en-e16E, arm^UAS.cSa

(Bauer et al., 2000)

Other

Statement

Reference

Myc^P0, pont[+]/pont^5.1 has wing phenotype

(Bellosta et al., 2005)

Myc^P0, pont[+]/pont^5.1 has wing margin phenotype

(Bellosta et al., 2005)

Myc^P0, pont^5.1 has wing phenotype

(Bellosta et al., 2005)

Myc^P0, pont^5.1 has wing margin phenotype

(Bellosta et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The low-penetrance "held-out" wing phenotype of brm²/+ adults is enhanced in a pont^5.1/+ background. Held-out wings are observed in 15% of double-heterozygous animals compared to 1.4% in brm²/+ mutants. Co-expression of pont^Scer\UAS.cDa under the control of Scer\GAL4^arm.PS in brm²/pont^5.1 animals reduces the penetrance of the wing phenotype to 1.7%.

Triple-heterozygous brm², pont^5.1, and rept³⁵ mutant adults have a "held-out" wing phenotype at a low penetrance (2.1%), quite similar to the penetrance observed in brm²/+ animals.

(Diop et al., 2008)

The defects of pont^5.1 rept³⁵ double mutants are similar to the single mutants, although the larvae do not survive for longer than 5 days after egg deposition.

(Bellosta et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64756

w^*; P{neoFRT}82B pont^5.1/TM3, Sb¹

Notes on Origin

Discoverer

Comments

Created by imprecise excision of the P{lacW}pont^S022905a insertion.

(Bauer et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pontin^5.1

(Prozzillo et al., 2021, Flegel et al., 2016)

pon^5.1

(Bauer et al., 2000)

pont^5.1

pont⁵¹

(Diop et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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