FlyBase Allele Report: Dmel\Myc[4]

General Information

Symbol

Dmel\Myc⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0175709

Feature type

allele

Associated gene

Dmel\Myc

Associated Insertion(s)

Carried in Construct

Also Known As

dm⁴, dMyc^dm4, dMyc⁴

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Pierce et al., 2004)

Progenitor genotype

Myc^BG02383

(Pierce et al., 2004)

Myc^BG00605

(Pierce et al., 2004)

Cytology

Description

The region between the insertion sited of P{GT1}dm^BG02383 and P{GT1}dm^BG00605 is deleted.

(Pierce et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Females heterozygous for dm⁴ have a significantly decreased mutation load and live longer than controls.

(Greer et al., 2013)

dm⁴ mutant larvae show impaired starvation induced autophagy of the fat body and midgut.

(Nagy et al., 2013)

Disc cells homozygous for dm⁴ proliferate poorly and form small, infrequently recovered clones.

(Neto-Silva et al., 2010)

dm⁴ germline stem cell (GSC) clones behave similarly to control GSC clones, and have normal division rates - GSC maintenance is not affected.

(Jin et al., 2008)

Eyes consisting of dm⁴ homozygous cells (induced using the ey-FLP method) contain a reduced number of small ommatidia.

(Steiger et al., 2008)

Homozygous clones in the eye generated using the ey-FLP system only ever occupy a small fraction of the mosaic eyes, in contrast to wild-type clones induced using this method.

(Steiger et al., 2008)

Embryonic development and hatching of larvae appears normal in dm⁴ hemizygous males. Nuclei of endoreplicating tissues in these animals (salivary gland, fat body, and gut tissue) are normal in size. However, within 24 hours of hatching, these larvae are smaller than wild-type, as are the cell nuclei in endoreplicating tissues. After molting to second instar, these larvae stop growing. Most die soon afterwards. At 72 hours after egg laying cell nuclei in endoreplicating tissues are much smaller than in wild-type, as is the overall size of the gut and salivary glands. Massively reduced BrdU incorporation into these abnormally small nuclei compared to controls suggests that their size reduction is due to reduced DNA replication.

(Pierce et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Myc⁴ has long lived | dominant phenotype, enhanceable by Scer\GAL4^{Tub.Switch.PU}/Sirt6^UAS.cTa

(Taylor et al., 2022)

Suppressed by

Statement

Reference

Myc⁴ has lethal | recessive phenotype, suppressible by Max¹

(Steiger et al., 2008)

Myc⁴ has decreased body size | larval stage phenotype, suppressible by Max¹

(Steiger et al., 2008)

Myc⁴ has lethal | recessive phenotype, suppressible by Mnt¹

(Steiger et al., 2008)

Myc⁴ has decreased body size | larval stage phenotype, suppressible by Mnt¹

(Steiger et al., 2008)

NOT Enhancer of

Statement

Reference

Myc⁴/Myc[+] is a non-enhancer of long lived | RU486 conditional phenotype of Scer\GAL4^{Tub.Switch.PU}, Sirt6^UAS.cTa

(Taylor et al., 2022)

Suppressor of

Statement

Reference

Myc⁴ is a suppressor | partially of abnormal wound healing | larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo, btl::Egfr^λ.UAS

(Li et al., 2020)

Other

Statement

Reference

Mnt¹, Myc⁴ has decreased body size | larval stage phenotype

(Steiger et al., 2008)

Mnt¹, Myc⁴ has decreased cell size | somatic clone phenotype

(Steiger et al., 2008)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Myc⁴ has wing disc | somatic clone phenotype, non-suppressible by Scer\GAL4^T80/Dronc^CARD.UAS

(Neto-Silva et al., 2010)

Myc⁴ has wing disc | somatic clone phenotype, non-suppressible by yki^UAS.cHa/Scer\GAL4^T80/Dronc^CARD.UAS

(Neto-Silva et al., 2010)

Suppressor of

Statement

Reference

Myc⁴ is a suppressor | partially of larval multidendritic class IV neuron phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo, btl::Egfr^λ.UAS

(Li et al., 2020)

Myc⁴ is a suppressor of wing disc | somatic clone phenotype of Scer\GAL4^Tub.PU, yki^UAS.cHa

(Neto-Silva et al., 2010)

Other

Statement

Reference

Mnt¹, Myc⁴ has embryonic/larval fat body phenotype

(Steiger et al., 2008, Steiger et al., 2008)

Mnt¹, Myc⁴ has wing disc | somatic clone phenotype

(Steiger et al., 2008)

Mnt¹, Myc⁴ has embryonic/larval salivary gland phenotype

(Steiger et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

dm⁴ mutant wing discs expressing yki^Scer\UAS.cHa under the control of Scer\GAL4^tub.PU display very small double-mutant clones.

Expression of Nc^{CARD.Scer\UAS} under the control of Scer\GAL4^T80 in dm⁴ mutant wing disc clones does not rescue clone size.

Expression of yki^Scer\UAS.cHa and Nc^{CARD.Scer\UAS} under the control of Scer\GAL4^T80 in dm⁴ mutant wing disc clones does not rescue clone size.

(Neto-Silva et al., 2010)

dm⁴ ; Mnt¹ double mutant clones in the fat body contain tiny cells that have minuscule nuclei.

(Steiger et al., 2008)

dm⁴ Max¹, dm⁴ Mnt¹ and dm⁴ Mnt¹ Max¹ larvae grow better and survive to a greater extent than dm⁴ single mutant larvae.

dm⁴ Mnt¹ larval fat body and salivary gland cells are severely stunted compared to controls.

dm⁴ Mnt¹ 72 hour old clones in the wing imaginal discs of wandering third instar larvae are significantly smaller than their wild-type twin spots and contain significantly smaller cells than their wild-type twin spots.

(Steiger et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Myc⁴/Myc[+] is rescued by Myc^GFP.FPTB

(Greer et al., 2013)

Myc⁴ is rescued by Myc^GFP.FPTB

(Greer et al., 2013)

Myc⁴ is rescued by Myc^tub.PG

(Pierce et al., 2004)

Partially rescued by

Myc⁴ is partially rescued by Myc^{WT.αTub84B.Tag:HA}

(Schwinkendorf and Gallant, 2009)

Myc⁴ is partially rescued by Myc^{ΔMB2.αTub84B.Tag:HA}

(Schwinkendorf and Gallant, 2009)

Myc⁴ is partially rescued by Myc^{ΔMB3.αTub84B.Tag:HA}

(Schwinkendorf and Gallant, 2009)

Myc⁴ is partially rescued by Scer\GAL4^αTub84B.PBb/Myc^{WT.UAS.Tag:HA}

(Steiger et al., 2008)

Not rescued by

Myc⁴ is not rescued by Myc^{ΔN.αTub84B.Tag:HA}

(Schwinkendorf and Gallant, 2009)

Comments

The P{αTub84B(FRT.Myc)GAL4.Bb}1 insertion is sufficient to rescue the lethality of hemizygous Myc⁴ males, but only partially rescues the sterility of homozygous Myc⁴ females.

(Gallant, 2016.4.26)

Expression of dm^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} rescues the dm⁴-associated lethality.

Expression of one copy of dm^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} restores the decreased mutation load and median lifespan in dm⁴ heterozygotes to wild-type levels.

(Greer et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

64769

Myc⁴/FM7i, P{ActGFP}JMR3

64758

w^* Myc⁴ Mnt¹ P{neoFRT}19A/FM7a

64766

P{hsFLP}12, w^* Myc⁴ P{αTub84B(FRT.Myc)GAL4.Bb}1/FM7i; P{UAS-GFP.U}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Myc4

(van den Berg et al., 2023)

Myc⁴

(Li et al., 2020, Gallant, 2016.4.26)

Myc[4]

(Nagy et al., 2013)

dMyc⁴

(Taylor et al., 2022, Zielke et al., 2022)

dMyc^dm4

(Marinho et al., 2011)

dm⁴

(Kim et al., 2015, de la Cova et al., 2014, Hovhanyan et al., 2014, Merzetti et al., 2013, Marinho et al., 2011, Neto-Silva et al., 2010, Rhiner et al., 2009, Schwinkendorf and Gallant, 2009, Steiger et al., 2008, Steiger et al., 2008, Orian et al., 2007)

dmyc4

(Rastegari et al., 2020)

dmyc^dm4

(Jin et al., 2008)

myc⁴

(Greer et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

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