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Moberg, K.H., Mukherjee, A., Veraksa, A., Artavanis-Tsakonas, S., Hariharan, I.K. (2004). The Drosophila F box protein archipelago regulates dMyc protein levels in vivo.  Curr. Biol. 14(11): 965--974.
FlyBase ID
FBrf0179346
Publication Type
Research paper
Abstract

The Myc oncoprotein is an important regulator of cellular growth in metazoan organisms. Its levels and activity are tightly controlled in vivo by a variety of mechanisms. In normal cells, Myc protein is rapidly degraded, but the mechanism of its degradation is not well understood.Here we present genetic and biochemical evidence that Archipelago (Ago), the F box component of an SCF-ubiquitin ligase and the Drosophila ortholog of a human tumor suppressor, negatively regulates the levels and activity of Drosophila Myc (dMyc) protein in vivo. Mutations in archipelago (ago) result in strongly elevated dMyc protein levels and increased tissue growth. Genetic interactions indicate that ago antagonizes dMyc function during development. Archipelago binds dMyc and regulates its stability, and the ability of Ago to bind dMyc in vitro correlates with its ability to inhibit dMyc accumulation in vivo.Our data indicate that archipelago is an important inhibitor of dMyc in developing tissues. Because archipelago can also regulate Cyclin E levels and Notch activity, these results indicate how a single F box protein can be responsible for the degradation of key components of multiple pathways that control growth and cell cycle progression.

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Note

Myc degradation: dancing with ubiquitin ligases.
Amati, 2004, Proc. Natl. Acad. Sci. U.S.A. 101(24): 8843--8844 [FBrf0188499]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Curr. Biol.
    Title
    Current Biology
    Publication Year
    1991-
    ISBN/ISSN
    0960-9822
    Data From Reference
    Alleles (12)
    Genes (9)
    Physical Interactions (4)
    Cell Lines (2)
    Insertions (1)
    Experimental Tools (1)
    Transgenic Constructs (3)