FB2025_05 , released December 11, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Myc, ago
FlyBase ID
FBig0000120297
Interaction Network
Interactions Browser links
Myc network
ago network
Reported Interactions
FBrf0179346-1.TAP.MS
Reference
Moberg et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc-unspecified
Participants
Corresponds to
Reported as
Role
Note
ago 

Ago

bait

TAP tag

Myc 

Myc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Moberg et al., 2004)
FBrf0179346-2.Y2H
Reference
Moberg et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ago 

Ago

bait

fused to LexA DNA-binding domain

ago 
Myc 

dMyc

prey

fused to unspecified activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ago 
interaction domain
sufficient binding region
aa 681-1325
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Moberg et al., 2004)
FBrf0179346-3.coIP.WB
Reference
Moberg et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ago 

Ago

bait

HA tag

ago 
ago 
ago 
Myc 

dMyc

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ago 
interaction domain
necessary binding region
aa 1195-1326

coordinates relative to ago-PA

ago 
interaction domain residue 1131
mutation disrupting interaction
aa 1131

G1131E, coordinates relative to ago-PA

ago 
F-box domain
mutation increasing interaction
aa 895-923

deletion of F-box, coordinates relative to ago-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Moberg et al., 2004)
FBrf0179346-4.coIP.WB
Reference
Moberg et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Myc 

dMyc

bait

FLAG tag

ago 

Ago

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Moberg et al., 2004)
FBrf0246146-4.coIP.WB
Reference
Thevenon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Myc 

dMYC

bait

FLAG tag

ago 

AGO

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The scny-ago interaction increases in the presence of Myc.

(Thevenon et al., 2020)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Thevenon et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (2)