FlyBase Gene Report: Dmel\pit

General Information

Symbol

Dmel\pit

Species

D. melanogaster

Name

pitchoune

Annotation Symbol

CG6375

Feature Type

protein_coding_gene

FlyBase ID

FBgn0266581

Gene Model Status

Current

Stock Availability

13 publicly available

Enzyme Name (EC)

RNA helicase (3.6.4.13)

Gene Summary

Probable RNA-dependent helicase. Functions in cell growth and proliferation. May have a role in ribosome biogenesis and, consequently, in protein biosynthesis. (UniProt, Q9VD51)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

94A1-94A1

Sequence location

3R:22,034,298..22,038,925 [+]

Recombination map

3-74

RefSeq locus

NT_033777 REGION:22034298..22038925

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR011545, InterPro:IPR044773

(InterPro Project Members, 2004-)

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR011545

(InterPro Project Members, 2004-)

enables RNA helicase activity

inferred from sequence or structural similarity with SGD:S000004903

(Lasko, 2000)

inferred from electronic annotation with InterPro:IPR014014, InterPro:IPR044773

(InterPro Project Members, 2004-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

inferred from biological aspect of ancestor with PANTHER:PTN002775131

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleolus

inferred from direct assay

(Zaffran et al., 1998)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleolus

inferred from biological aspect of ancestor with PANTHER:PTN002775131

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

DEAD-BOX RNA HELICASES

Protein Family (UniProt)

Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily. (Q9VD51)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

RNA helicase activity

ATP + H2O = ADP + phosphate + H(+) (3.6.4.13)

Summaries

Gene Group (FlyBase)

DEAD-BOX RNA HELICASES -: DEAD-box RNA helicases belong to helicase superfamily 2 (SF2). They are characterized by the presence of nine conserved helicase motifs, including the DEAD sequence in motif II. (Adapted from PMID:21509200.)

Protein Function (UniProtKB)

Probable RNA-dependent helicase. Functions in cell growth and proliferation. May have a role in ribosome biogenesis and, consequently, in protein biosynthesis.

(UniProt, Q9VD51)

Summary (Interactive Fly)

DEAD-box RNA helicase - a target of myc suggesting that myc might cell proliferation by activating genes that are required in protein biosynthesis

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\pit for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VD51)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

pit-RA

FBtr0084175

NM_079722

2270

680

pit-RB

FBtr0084174

NM_169991

2324

680

pit-RC

FBtr0346345

NM_001300530

2803

680

Additional Transcript Data and Comments

Reported size (kB)

2.4 (northern blot)

(Zaffran et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

pit-PA

FBpp0083573

76.9

680

8.19

FBpp0083572

76.9

680

8.19

FBpp0312062

76.9

680

8.19

Q9VD51

NP_001287459

AHN57458

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

680 aa isoforms: pit-PA, pit-PB, pit-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

772 (aa); 75 (kD predicted)

(Zaffran et al., 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pit using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

pit_1
pit_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.46

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

pre-blastoderm stage

organism | ubiquitous

(Zaffran et al., 1998)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

gastrula stage

mesoderm

(Zaffran et al., 1998)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11

embryonic/larval salivary gland | precursor

(Zaffran et al., 1998)

cercus

(Zaffran et al., 1998)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

fat body primordium

Comment: reported as fat body specific anlage

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

labial sensory complex primordium

(Fisher et al., 2012)

labral sensory complex primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

proventriculus primordium

(Fisher et al., 2012)

salivary gland body primordium

Comment: reported as salivary gland primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 17

anterior midgut primordium

(Zaffran et al., 1998)

posterior midgut primordium

(Zaffran et al., 1998)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic proventriculus

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

embryonic stage 16

ovarian sheath of embryonic/larval midgut

(Zaffran et al., 1998)

Malpighian tubule

(Zaffran et al., 1998)

embryonic/larval midgut

(Zaffran et al., 1998)

gonadal sheath

(Zaffran et al., 1998)

ovarian sheath of embryonic/larval hindgut

(Zaffran et al., 1998)

larval stage

imaginal disc | ubiquitous

(Zaffran et al., 1998)

adult stage & oogenesis

germarium

(Zaffran et al., 1998)

oogenesis stage S4 -- S14 & adult stage | female

egg chamber

(Zaffran et al., 1998)

Additional Descriptive Data

pit transcripts are uniformly distributed in pre-blastoderm embryos. At gastrulation, pit is expressed in the invaginating mesoderm. At stage 11, expression is observed in the mesoderm in the anterior and posterior midgut precursors and in the salivary gland precursors. Strong expression is also seen in the anal plate. In later stages, expression is limited to the midgut, the Malpighian tubules and the mesodermal sheath of gonads. At stage 16 pit transcripts are observed in the entire epithelial sheet of the intestine. In ovaries, pit transcripts are observed in the germarium and then in egg chambers from stage 4 on. Ubiquitous expression is observed in imaginal discs.

(Zaffran et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleolus

inferred from direct assay

(Zaffran et al., 1998)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\pit in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\pit

View exonic expression by tissue for Dmel\pit

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266581

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42595/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266581

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025140

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266581

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6375

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of pit

Transgenic constructs containing regulatory region of pit

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)e-BS2

(Zaffran et al., 1998)

Df(3R)Exel6188

(Ryder, 2004.4.26)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with pit¹⁰

pit^mod.PZ

lethal, with pit^mod.PZ

pit¹⁰

lethal | recessive

pit¹⁰

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

pit^GD11905

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

pit^GD11905

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

pit^GD11905

partially lethal, with Scer\GAL4^tin.CΔ4

pit^GD11905

viable

Sterility

Allele

fertile

pit^f06954

Other Phenotypes

Allele

abnormal developmental rate

abnormal developmental rate | larval stage, with pit¹⁰

pit^mod.PZ

abnormal developmental rate | larval stage, with pit^mod.PZ

pit¹⁰

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

pit^GD11905

abnormal size | larval stage

pit¹⁰

decreased cell number | larval stage

pit¹⁰

decreased cell number | larval stage | somatic clone

pit¹⁰

decreased cell size | larval stage

pit¹⁰

decreased occurrence of cell division | larval stage

pit¹⁰

decreased size | adult stage

pit^Ebox

hyperplasia | larval stage, with Scer\GAL4^en-e16E

pit^UAS.cZa

increased cell death | larval stage, with Scer\GAL4^en-e16E

pit^UAS.cZa

increased occurrence of cell division | larval stage, with Scer\GAL4^en-e16E

pit^UAS.cZa

some die during pupal stage, with Scer\GAL4^pnr-MD237

pit^GD11905

Phenotype manifest in

Allele

adult midgut progenitor cell | larval stage

pit¹⁰

embryonic/larval midgut | embryonic stage

pit¹⁰

embryonic/larval salivary gland | embryonic stage

pit¹⁰

imaginal tissue | larval stage

pit¹⁰

scutellar bristle

pit^Ebox

thorax | larval stage | somatic clone

pit¹⁰

wing disc, with Scer\GAL4^en-e16E

pit^UAS.cZa

wing disc | larval stage | somatic clone

pit¹⁰

wing disc posterior compartment, with Scer\GAL4^en-e16E

pit^UAS.cZa

wing disc posterior compartment | larval stage, with Scer\GAL4^en-e16E

pit^UAS.cZa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (26)

Hsap\DDX18

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (22)

Rnor\Ddx18

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (25)

Mmus\Ddx18

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (18)

Xtro\dpp10

5 of 13

Yes

Xtro\ddx10

2 of 13

Xtro\ddx18

2 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (15)

Drer\ddx18

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (25)

Cele\B0511.6

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (25)

Agam\AgaP_AGAP008601

12 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (45)

Atha\AT3G18600

13 of 13

Yes

Atha\AT5G65900

13 of 13

Yes

Atha\AT1G63250

2 of 13

Yes

Atha\AT1G71280

2 of 13

Atha\AT1G71370

2 of 13

Atha\AT2G07750

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Atha\PDE340

2 of 13

Yes

Atha\RH1

2 of 13

Atha\STRS2

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (20)

Scer\HAS1

13 of 13

Yes

Scer\MSS116

3 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (12)

Spom\has1

12 of 12

Yes

Spom\SPBC691.04

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (4)

Ecol\dbpA

1 of 11

Yes

Ecol\hsdR

1 of 11

Yes

Ecol\radD

1 of 11

Yes

Ecol\rhlE

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:pit. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (31)

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

ais

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

Rs1

3 of 13

bel

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

vas

2 of 13

abs

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DDX18; DEAD-box helicase 18

14 of 14

606355

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

pit - CG2691

experimental knowledge based

(Guruharsha et al., 2011)

pit - CG32344

experimental knowledge based

(Guruharsha et al., 2011)

pit - CG5033

experimental knowledge based

(Guruharsha et al., 2011)

pit - CG6937

experimental knowledge based

(Guruharsha et al., 2011)

pit - CG8939

experimental knowledge based

(Guruharsha et al., 2011)

pit - Ifrd1

experimental knowledge based

(Guruharsha et al., 2011)

pit - Noc3

experimental knowledge based

(Guruharsha et al., 2011)

pit - Non1

experimental knowledge based

(Guruharsha et al., 2011)

pit - Nop56

experimental knowledge based

(Guruharsha et al., 2011)

pit - Ns1

experimental knowledge based

(Guruharsha et al., 2011)

pit - Ns2

experimental knowledge based

(Guruharsha et al., 2011)

pit - Nsun1

experimental knowledge based

(Guruharsha et al., 2011)

pit - Rbm13

experimental knowledge based

(Guruharsha et al., 2011)

pit - RpL7-like

experimental knowledge based

(Guruharsha et al., 2011)

pit - Rs1

experimental knowledge based

(Guruharsha et al., 2011)

pit - Srp72

experimental knowledge based

(Guruharsha et al., 2011)

pit - Su(var)205

proximity-dependent biotin identification, western blot, pull down, anti bait coimmunoprecipitation

(Rajshekar et al., 2025)

pit - bdwf

experimental knowledge based

(Guruharsha et al., 2011)

pit - foxo

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

pit - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

pit - l(1)G0020

experimental knowledge based

(Guruharsha et al., 2011)

pit - l(2)k09848

experimental knowledge based

(Guruharsha et al., 2011)

pit - nop5

experimental knowledge based

(Guruharsha et al., 2011)

pit - pav

experimental knowledge based

(Guruharsha et al., 2011)

pit - pths

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266581

MIST (protein-protein) - An integrated Molecular Interaction Database

42595

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-74

Cytogenetic map

94A1-94A1

Sequence location

3R:22,034,298..22,038,925 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

94A1-94A1

Limits computationally determined from genome sequence between P{PZ}tsl⁰⁰⁶¹⁷ and P{lacW}how^j5B5&P{EP}EP738

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

93F-93F

(Zaffran, 1997.1.9)

93F-93F

(determined by in situ hybridisation)

(Zaffran et al., 1998, Zaffran et al., 1995)

Experimentally Determined Recombination Data

Location

3-74

(FlyBase, 2016)

3-76.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

605946

w^*; P{pit-EGFP}attP2

43984

y¹ v¹; P{TRiP.GLC01465}attP2

80368

y¹ v¹; P{TRiP.HMC06604}attP40

19026

w¹¹¹⁸; PBac{WH}Fadd^f06954 pit^f06954

62741

w¹¹¹⁸; PBac{IT.GAL4}Fadd^0258-G4 pit^0258-G4

20638

y¹ w^67c23; P{EPgy2}Fadd^EY10870 pit^EY10870

20904

y¹ w^67c23; P{EPgy2}Fadd^EY14017 pit^EY14017

FlyORF

F001440

M{UAS-pit.ORF}ZH-86Fb

10944

v106078

v27600

w¹¹¹⁸; P{GD11905}v27600

v318732

PBac{fTRG01035.sfGFP-TVPTBF}VK00002

More stocks available...

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (45)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_LD46167

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0266581

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Rajshekar et al., 2025)

polyclonal

(Zaffran et al., 1998)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene in combination with dsRNA against gig results in suppression of the final average cell size, with it being closer to the control average cell size, rather than the average cell size resulting from knockdown of the candidate gene alone.

(Guertin et al., 2006)

dsRNA made from templates generated with primers directed against pit that is transfected into S2 treated with Listeria monocytogenes reveals pit to be involved in Listeria monocytogenes intracellular growth and vacuolar escape.

(Cheng et al., 2005)

pit is required for cell growth and proliferation.

(Zaffran et al., 1998)

Relationship to Other Genes

Source for database merge of

Source for merge of: pit Hel93F

(FlyBase, 1996-)

Additional comments

scaRNA:mgU5-38 is encoded in an intron of pit.

(Graveley et al., 2011)

Comparison of the molecular map in Figure 2 of FBrf0093800 with the corresponding region of the release 5.53 genome, and the presence of a helicase domain in pit, indicates that "hel93F" in that Figure corresponds to pit ( date:131030 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

The gene is named "pitchoune" (meaning "small" in the Provencal language) after the phenotype of mutant larvae, which do not grow beyond the first instar stage but can survive for as long as 7-10 days.

(Zaffran et al., 1998)

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG6375

(Çiçek et al., 2016, Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Cakouros et al., 2008, Guertin et al., 2006)

DDX18

(Fortuna et al., 2021)

DmRH15

(Boudet et al., 2001)

Hel93F

PIT

(Boudet et al., 2001)

Pit

(Ogienko et al., 2024, Falahati and Wieschaus, 2017, Andersen et al., 2010)

hel93F

(Zaffran et al., 1997)

pit

(Lee et al., 2024, Sureka and Mishra, 2021, Taschuk et al., 2020, Clandinin and Owens, 2016-, Kučerová et al., 2016, Sarov et al., 2016, Moy et al., 2014, Schertel et al., 2013, Friedman et al., 2011, Graveley et al., 2011, Furrer et al., 2010, Rhodes et al., 2010, FlyBase Curators, 2008-, Benassayag et al., 2005)

Name Synonyms

Helicase at 93F

(Zaffran et al., 1995)

pitchoune

(Dyson et al., 2025, Zielke et al., 2022, Fortuna et al., 2021, Ulvila et al., 2010, Benassayag et al., 2005)

Secondary FlyBase IDs

FBgn0025140
FBgn0015236

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42595

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VD51

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VD51

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4LHF7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VD51

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266581

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42595/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266581

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266581

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6375

MARRVEL_MODEL - MARRVEL (model organism gene)

42595

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0266581

DroID - A comprehensive database of gene and protein interactions.

FBgn0266581

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

pit_1
pit_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025140

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0266581

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6375

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000825

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

42595

References (86)

The following external sites may use different assemblies or annotations than FlyBase.

-0.46

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