Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Gene model reviewed during 5.56
2.4 (northern blot)
772 (aa); 75 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pit using the Feature Mapper tool.
pit transcripts are uniformly distributed in pre-blastoderm embryos. At gastrulation, pit is expressed in the invaginating mesoderm. At stage 11, expression is observed in the mesoderm in the anterior and posterior midgut precursors and in the salivary gland precursors. Strong expression is also seen in the anal plate. In later stages, expression is limited to the midgut, the Malpighian tubules and the mesodermal sheath of gonads. At stage 16 pit transcripts are observed in the entire epithelial sheet of the intestine. In ovaries, pit transcripts are observed in the germarium and then in egg chambers from stage 4 on. Ubiquitous expression is observed in imaginal discs.
GBrowse - Visual display of RNA-Seq signalsView Dmel\pit in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: pit Hel93F
dsRNA made from templates generated with primers directed against this gene in combination with dsRNA against gig results in suppression of the final average cell size, with it being closer to the control average cell size, rather than the average cell size resulting from knockdown of the candidate gene alone.
dsRNA made from templates generated with primers directed against pit that is transfected into S2 treated with Listeria monocytogenes reveals pit to be involved in Listeria monocytogenes intracellular growth and vacuolar escape.
The gene is named "pitchoune" (meaning "small" in the Provencal language) after the phenotype of mutant larvae, which do not grow beyond the first instar stage but can survive for as long as 7-10 days.