FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Hel25E
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General Information
Symbol
Dmel\Hel25E
Species
D. melanogaster
Name
Helicase at 25E
Annotation Symbol
CG7269
Feature Type
protein_coding_gene
FlyBase ID
FBgn0014189
Gene Model Status
Current
Stock Availability
12 publicly available
Enzyme Name (EC)
RNA helicase (3.6.4.13)
Gene Summary
Helicase at 25E (Hel25E) encodes the UAP56 RNA helicase in the DECD subfamily of DEAD-box helicases. It is an enhancer of position-effect variegation, and contributes to mRNA splicing, nuclear export, and piRNA biosynthesis. The product of Hel25E binds heterochromatic cluster transcripts, along with the product of vas, to organize a piRNA-processing compartment that spans the nuclear envelope to silence transposon transcripts. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

UAP56, hel, WM6, l(2)k11511, l(2)gdh-12

Key Links
Genomic Location
Cytogenetic map
25E6-25E6
Sequence location
Recombination map
2-17
RefSeq locus
NT_033779 REGION:5539326..5542314
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (16 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables piRNA binding
inferred from direct assay
(Zhang et al., 2012)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR011545
(InterPro Project Members, 2004-)
enables mRNA binding
inferred from biological aspect of ancestor with PANTHER:PTN002776867
(GO Reference Genome Project, 2011-)
enables nucleic acid binding
inferred from electronic annotation with InterPro:IPR011545
(InterPro Project Members, 2004-)
enables RNA helicase activity
inferred from biological aspect of ancestor with PANTHER:PTN002774595
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR014014
(InterPro Project Members, 2004-)
inferred from sequence or structural similarity with SGD:S000002242
(Lasko, 2000)
Biological Process (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in chromatin remodeling
inferred from mutant phenotype
(Eberl et al., 1997)
involved_in mRNA export from nucleus
inferred from mutant phenotype
(Gatfield et al., 2001)
involved_in positive regulation of piRNA transcription
inferred from mutant phenotype
(Zhang et al., 2012)
involved_in regulation of alternative mRNA splicing, via spliceosome
inferred from mutant phenotype
(Katzenberger et al., 2009)
inferred from high throughput mutant phenotype
(Park et al., 2004)
involved_in secondary piRNA processing
inferred from mutant phenotype
(Zhang et al., 2012)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in mRNA export from nucleus
inferred from biological aspect of ancestor with PANTHER:PTN002776405
(GO Reference Genome Project, 2011-)
involved_in mRNA splicing, via spliceosome
inferred from sequence or structural similarity with SGD:S000002242
(Mount and Salz, 2000)
inferred from biological aspect of ancestor with PANTHER:PTN002776405
(GO Reference Genome Project, 2011-)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
is_active_in nucleus
inferred from direct assay
(Zhang et al., 2012)
located_in nucleus
inferred from direct assay
(Eberl et al., 1997)
is_active_in P granule
inferred from direct assay
(Zhang et al., 2012)
colocalizes_with polytene chromosome
inferred from direct assay
(Eberl et al., 1997)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
located_in nucleus
inferred by curator from GO:0000381
(Park et al., 2004)
part_of spliceosomal complex
inferred from sequence or structural similarity with SGD:S000002242
(Mount and Salz, 2000)
part_of transcription export complex
inferred from sequence or structural similarity with UniProtKB:Q13838
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the DEAD box helicase family. DECD subfamily. (Q27268)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
RNA helicase activity
ATP + H2O = ADP + phosphate + H(+) (3.6.4.13)
Summaries
Gene Snapshot
Helicase at 25E (Hel25E) encodes the UAP56 RNA helicase in the DECD subfamily of DEAD-box helicases. It is an enhancer of position-effect variegation, and contributes to mRNA splicing, nuclear export, and piRNA biosynthesis. The product of Hel25E binds heterochromatic cluster transcripts, along with the product of vas, to organize a piRNA-processing compartment that spans the nuclear envelope to silence transposon transcripts. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
TRANSCRIPTION EXPORT COMPLEX -
The Transcription and export (TREX) complex is a multisubunit complex, which acts throughout RNA biogenesis to couple transcription with nuclear export. (Adapted from FBrf0233979 and PMID:27679854).
DEAD-BOX RNA HELICASES -
DEAD-box RNA helicases belong to helicase superfamily 2 (SF2). They are characterized by the presence of nine conserved helicase motifs, including the DEAD sequence in motif II. (Adapted from PMID:21509200.)
Protein Function (UniProtKB)
Required for mRNA export out of the nucleus. Probable RNA helicase that may regulate entry into mitosis by down-regulating the expression of other genes whose activity may be rate-limiting for entry into mitosis during embryogenesis. Binds to salivary gland chromosomes and modifies position effect variegation. Promotes an open chromatin structure that favors transcription during development by regulating the spread of heterochromatin.
(UniProt, Q27268)
Summary (Interactive Fly)

DExH/D RNA helicase required in the nucleus for mRNA export - required in the cytoplasm for remodeling the transacting factors that dictate mRNA cytoplasmic destination

Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Hel25E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q27268)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Hel25E-RA
FBtr0079124
NM_164646
1596
424
Hel25E-RB
FBtr0079125
NM_164647
1657
424
Hel25E-RC
FBtr0079123
NM_164648
1760
424
Hel25E-RD
FBtr0331676
NM_001273171
2270
305
Additional Transcript Data and Comments
Reported size (kB)

1.8, 1.6 (northern blot)

(Eberl et al., 1997)

1.6 (longest cDNA)

(Warbrick and Glover, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Hel25E-PA
FBpp0078755
48.7
424
5.37
Q27268
NP_723089
AAF52261
Hel25E-PB
FBpp0078756
48.7
424
5.37
Q27268
NP_723090
AAN10544
Hel25E-PC
FBpp0078754
48.7
424
5.37
Q27268
NP_723091
AAN10545
Hel25E-PD
FBpp0304066
34.6
305
6.78
M9PC90
NP_001260100
AGB92636
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

424 aa isoforms: Hel25E-PA, Hel25E-PB, Hel25E-PC
Additional Polypeptide Data and Comments
Reported size (kDa)

424 (aa); 47 (kD observed)

(Eberl et al., 1997)

424 (aa)

(Warbrick and Glover, 1994)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the spliceosome (Probable). Interacts with the exon junction complex.

(UniProt, Q27268)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hel25E using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.43

Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Eberl et al., 1997)
gastrula stage
mitotic domain
(Eberl et al., 1997)
larval stage
embryonic/larval salivary gland
(Eberl et al., 1997)
adult stage & oogenesis
follicle cell
(Eberl et al., 1997)
nurse cell
(Eberl et al., 1997)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

In the larval salivary gland, Hel25E protein associates with the polytene chromosomes. In ovaries, staining is seen in nurse cell and follicle cell nuclei. Confocal microscopy of cycle 14 embyros double-labeled with antibodies against Hel25E and betaTub60D shows that Hel25E disappears from within mitotic domains, although it is present in the nuclei of the other cells at the surface of the embryo.

(Eberl et al., 1997)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
is_active_in nucleus
inferred from direct assay
(Zhang et al., 2012)
located_in nucleus
inferred from direct assay
(Eberl et al., 1997)
is_active_in P granule
inferred from direct assay
(Zhang et al., 2012)
colocalizes_with polytene chromosome
inferred from direct assay
(Eberl et al., 1997)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Hel25E in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 9 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Hel25E
Transgenic constructs containing regulatory region of Hel25E
Aberrations (Deficiencies and Duplications) ( 25 )
Inferred from experimentation ( 25 )
Gene disrupted in
Df(2L)BSC169
(Christensen and Cook, 2006.7.11)
Df(2L)BSC810
(Christensen et al., 2009.5.6)
Df(2L)cl1
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl2
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl7
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl-h1
(Eberl et al., 1997, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl-h2
(Eberl et al., 1997, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl-h3
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl-h4
(Eberl et al., 1997, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)Exel6012
(Ryder, 2004.4.26)
Df(2L)Exel7023
(Meignin and Davis, 2008, Ryder, 2004.4.26)
Df(2L)Gpdh1-A
(Kotarski et al., 1983)
Df(2L)sc19-4
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-12
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Ts(YLt;2Lt)B137+Ts(YLt;2Rt)B236
(Kotarski et al., 1983)
Gene not disrupted in
Df(2L)2802
(Kotarski et al., 1983)
Df(2L)Gpdh1-75
(Kotarski et al., 1983)
Df(2L)Gpdh1-78
(Kotarski et al., 1983)
Df(2L)sc19-1
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-5
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-6
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-7
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-8
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)tkv2
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)tkv3
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Inferred from location ( 7 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (19)
Hsap\DDX39A
13 of 14
Yes
Yes
1  
Hsap\DDX39B
13 of 14
Yes
Yes
0  
Hsap\DDX6
1 of 14
No
No
Hsap\DDX19A
1 of 14
No
No
Hsap\DDX19B
1 of 14
No
No
Hsap\DDX20
1 of 14
No
No
Hsap\DDX23
1 of 14
No
No
Hsap\DDX25
1 of 14
No
No
Hsap\DDX27
1 of 14
No
No
Hsap\DDX41
1 of 14
No
No
Hsap\DDX42
1 of 14
No
No
Hsap\DDX43
1 of 14
No
No
Hsap\DDX46
1 of 14
No
No
Hsap\DDX47
1 of 14
No
No
Hsap\DDX49
1 of 14
No
No
Hsap\DDX53
1 of 14
No
No
Hsap\EIF4A1
1 of 14
No
No
Hsap\EIF4A2
1 of 14
No
No
5  
Hsap\EIF4A3
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (10)
Rnor\Ddx39a
13 of 14
Yes
Yes
Rnor\Ddx39b
13 of 14
Yes
Yes
Rnor\Ddx6
1 of 14
No
No
Rnor\Ddx19b
1 of 14
No
No
Rnor\Ddx20
1 of 14
No
No
Rnor\Ddx42
1 of 14
No
No
Rnor\Ddx46
1 of 14
No
No
Rnor\Eif4a1
1 of 14
No
No
Rnor\Eif4a2
1 of 14
No
No
Rnor\Eif4a3
1 of 14
No
No
Mus musculus (laboratory mouse) (12)
Mmus\Ddx39b
13 of 14
Yes
Yes
Mmus\Ddx39a
12 of 14
No
Yes
Mmus\Ddx6
1 of 14
No
No
Mmus\Ddx19a
1 of 14
No
No
Mmus\Ddx20
1 of 14
No
No
Mmus\Ddx42
1 of 14
No
No
Mmus\Ddx46
1 of 14
No
No
Mmus\Eif4a1
1 of 14
No
No
Mmus\Eif4a2
1 of 14
No
No
Mmus\Eif4a3l1
1 of 14
No
No
Mmus\Eif4a3l2
1 of 14
No
No
Mmus\Eif4a3
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (10)
Xtro\ddx39a
7 of 13
Yes
Yes
Xtro\ddx39b
7 of 13
Yes
Yes
Xtro\cacna2d1
1 of 13
No
No
Xtro\cntnap4
1 of 13
No
No
Xtro\ddx6
1 of 13
No
No
Xtro\ddx27
1 of 13
No
No
Xtro\ddx41
1 of 13
No
No
Xtro\ddx42
1 of 13
No
No
Xtro\ddx59
1 of 13
No
No
Xtro\eif4a3.1
1 of 13
No
No
Danio rerio (Zebrafish) (13)
Drer\ddx39aa
13 of 14
Yes
Yes
Drer\ddx39ab
13 of 14
Yes
Yes
Drer\ddx39b
13 of 14
Yes
Yes
Drer\ddx6
1 of 14
No
No
Drer\ddx19b
1 of 14
No
No
Drer\ddx20
1 of 14
No
No
Drer\ddx42
1 of 14
No
No
Drer\ddx46
1 of 14
No
No
Drer\ddx61
1 of 14
No
No
Drer\eif4a1a
1 of 14
No
No
Drer\eif4a1b
1 of 14
No
No
Drer\eif4a2
1 of 14
No
No
Drer\eif4a3
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (16)
Cele\hel-1
12 of 14
Yes
Yes
Cele\C46F11.4
1 of 14
No
No
Cele\cgh-1
1 of 14
No
No
Cele\ddx-17
1 of 14
No
No
Cele\ddx-19
1 of 14
No
No
Cele\ddx-23
1 of 14
No
No
Cele\ddx-27
1 of 14
No
No
Cele\ddx-46
1 of 14
No
No
Cele\F33D11.10
1 of 14
No
No
Cele\F57B9.3
1 of 14
No
No
Cele\H20J04.4
1 of 14
No
No
Cele\inf-1
1 of 14
No
No
Cele\mel-46
1 of 14
No
No
Cele\T26G10.1
1 of 14
No
No
Cele\Y65B4A.6
1 of 14
No
No
Cele\Y94H6A.5
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (14)
Agam\AgaP_AGAP009075
12 of 12
Yes
Yes
Agam\AgaP_AGAP003089
1 of 12
No
No
Agam\AgaP_AGAP003397
1 of 12
No
No
Agam\AgaP_AGAP003663
1 of 12
No
No
Agam\AgaP_AGAP004351
1 of 12
No
No
Agam\AgaP_AGAP004711
1 of 12
No
No
Agam\AgaP_AGAP005351
1 of 12
No
No
Agam\AgaP_AGAP005391
1 of 12
No
No
Agam\AgaP_AGAP005652
1 of 12
No
No
Agam\AgaP_AGAP009135
1 of 12
No
No
Agam\AgaP_AGAP009808
1 of 12
No
No
Agam\AgaP_AGAP009863
1 of 12
No
No
Agam\AgaP_AGAP010656
1 of 12
No
No
Agam\AgaP_AGAP012523
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (29)
Atha\UAP56a
13 of 13
Yes
Yes
Atha\UAP56b
12 of 13
No
Yes
Atha\AT1G20920
1 of 13
No
No
Atha\AT1G51380
1 of 13
No
No
Atha\AT1G72730
1 of 13
No
No
Atha\AT2G33730
1 of 13
No
No
Atha\AT2G45810
1 of 13
No
No
Atha\AT2G47330
1 of 13
No
No
Atha\AT3G02065
1 of 13
No
No
Atha\AT3G06480
1 of 13
No
No
Atha\AT3G09620
1 of 13
No
No
Atha\AT3G61240
1 of 13
No
No
Atha\AT4G16630
1 of 13
No
No
Atha\AT4G33370
1 of 13
No
No
Atha\AT5G14610
1 of 13
No
No
Atha\AT5G51280
1 of 13
No
No
Atha\AT5G60990
1 of 13
No
No
Atha\AT5G63120
1 of 13
No
No
Atha\DRH1
1 of 13
No
No
Atha\EIF4A1
1 of 13
No
No
Atha\EIF4A-2
1 of 13
No
No
Atha\EIF4A-III
1 of 13
No
No
Atha\emb1138
1 of 13
No
No
Atha\LOS4
1 of 13
No
No
Atha\PMH1
1 of 13
No
No
Atha\PMH2
1 of 13
No
No
Atha\RH8
1 of 13
No
No
Atha\RH20
1 of 13
No
No
Atha\RH36
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (12)
Scer\SUB2
12 of 13
Yes
Yes
Scer\DBP2
1 of 13
No
No
Scer\DBP3
1 of 13
No
No
Scer\DBP5
1 of 13
No
No
Scer\DED1
1 of 13
No
No
Scer\DHH1
1 of 13
No
No
Scer\DRS1
1 of 13
No
No
Scer\FAL1
1 of 13
No
No
Scer\PRP5
1 of 13
No
No
Scer\RRP3
1 of 13
No
No
Scer\TIF1
1 of 13
No
No
Scer\TIF2
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (7)
Spom\uap56
12 of 12
Yes
Yes
Spom\dbp2
1 of 12
No
No
Spom\dbp3
1 of 12
No
No
Spom\prp11
1 of 12
No
No
Spom\SPAC1F5.10
1 of 12
No
No
Spom\SPAC1006.07
1 of 12
No
No
Spom\ste13
1 of 12
No
No
Escherichia coli (enterobacterium) (1)
Ecol\deaD
1 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Hel25E. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (30)
abs
3 of 13
bel
3 of 13
CG7483
3 of 13
CG10077
3 of 13
CG10333
3 of 13
Dbp80
3 of 13
Gem3
3 of 13
mahe
3 of 13
Rm62
3 of 13
vas
3 of 13
ais
2 of 13
CG6418
2 of 13
CG7878
2 of 13
CG14443
2 of 13
eIF4A
2 of 13
me31B
2 of 13
Prp5
2 of 13
pths
2 of 13
Rs1
2 of 13
CG5800
1 of 13
CG8611
1 of 13
CG9143
1 of 13
CG9630
1 of 13
CG32344
1 of 13
Dbp21E2
1 of 13
Dbp45A
1 of 13
Dbp73D
1 of 13
Ddx1
1 of 13
Ddx56
1 of 13
pit
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 2 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    DDX39A; DExD-box helicase 39A
    13 of 14
    619906
         
        DDX39B; DExD-box helicase 39B
        13 of 14
        142560
             
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            Hel25E - Akt
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Vinayagam et al., 2016)
            Hel25E - Arf1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Arl8
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Atpα
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Boot
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (ElMaghraby et al., 2019)
            Hel25E - CCT3
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - CCT6
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - CG1416
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - CtsL1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Droj2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - FASN1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Gfat2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Gp93
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Hpr1
            anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot
            (Zhang et al., 2018)
            Hel25E - KdelR
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Kdsr
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Ldh
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Mcad
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Mec2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Nxf3
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (ElMaghraby et al., 2019)
            Hel25E - Ost1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Ost48
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - OstΔ
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Rab1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Rab2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Rab5
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Rpt1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - S6k
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Vinayagam et al., 2016)
            Hel25E - SERCA
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - ScsβA
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - ScsβG
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - SsRβ
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Tapδ
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Tecr
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - UQCR-C2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - Vha68-2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - cathD
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - mEFTu1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - mge
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - sima
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Vinayagam et al., 2016)
            Hel25E - spidey
            experimental knowledge based
            (Guruharsha et al., 2011)
            Hel25E - tho2
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Zhang et al., 2018)
            Hel25E - thoc5
            anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry
            (Zhang et al., 2018, Hur et al., 2016)
            Hel25E - thoc6
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Zhang et al., 2018)
            Hel25E - thoc7
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Zhang et al., 2018)
            Hel25E - wal
            experimental knowledge based
            (Guruharsha et al., 2011)
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            peb
            suppressible
            Hel25E
            (Wilk et al., 2004)
            External Data
            Subunit Structure (UniProtKB)
            Component of the spliceosome (Probable). Interacts with the exon junction complex.
            (UniProt, Q27268 )
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Linkouts
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2L
            Recombination map
            2-17
            Cytogenetic map
            25E6-25E6
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            25E6-25E6
            Limits computationally determined from genome sequence between P{lacW}vrik05901&P{PZ}cype03771 and P{lacW}Hel25Ek11511
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            25F-25F
            (Warbrick, 1994.6.20)
            25E-25E
            (determined by in situ hybridisation)
            (Lorenz, 1995.10.18)
            25F-25F
            (determined by in situ hybridisation)
            (Warbrick and Glover, 1994)
            Experimentally Determined Recombination Data
            Location

            2-17

            (FlyBase, 2016)

            2-18.5

            (Comeron et al., 2012)
            Left of (cM)
            (Kotarski et al., 1983)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (12)
            Genomic Clones (15)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (195)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Eberl et al., 1997)
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments

                RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

                (Goshima et al., 2007)

                Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.

                (Nybakken et al., 2005)

                Hel25E encodes a putative RNA helicase that contains an unusual variant (DECD) of the DEAD motif common to these proteins.

                (Eberl et al., 1997)

                Deletion or mutation of the Hel25E locus enhances position effect variegation of w.

                (Eberl et al., 1997)

                Hel25E gene can influence position effect variegation (PEV). Loss of one or two copies enhances PEV of a variegating allele of the w gene, complete removal of Hel25E is lethal.

                (Lorenz et al., 1995)

                S.pombe Spom\wee1 Spom\mik1 double mutants are functionally complemented by Hel25E.

                (Warbrick and Glover, 1994)
                Relationship to Other Genes
                Source for database merge of
                Additional comments
                Nomenclature History
                Source for database identify of

                Source for identity of: Hel25E CG7269

                (Skupski, 1999.11)
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (26)
                Reported As
                Symbol Synonym
                CG7269
                (National Institute of Genetics Fly Stocks, 2022-, Kolkhof et al., 2017, Boltengagen et al., 2016, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Coolon et al., 2013, Ni et al., 2010.12.1, Yamamoto-Hino et al., 2010, Ardehali et al., 2009, Katzenberger et al., 2009, Keleman et al., 2009.8.5, Hughes et al., 2008, Goshima et al., 2007, Nybakken et al., 2005, Fulton et al., 2001, Mount and Salz, 2000)
                Dbp25F
                DmRH4
                (Boudet et al., 2001)
                HEL
                (Gatfield et al., 2001)
                HEL25E
                (Paraskevopoulos and McGuigan, 2021, Ellis et al., 2015)
                Hel
                Hel25E
                (Dyson et al., 2025, Choudhury et al., 2024, Nagata and Igaki, 2024, Wang et al., 2024, Stanhope et al., 2023, National Institute of Genetics Fly Stocks, 2022-, Azpurua et al., 2021, Malik et al., 2021, Sureka and Mishra, 2021, van der Graaf et al., 2021, Baker, 2020, Taschuk et al., 2020, Kneuss et al., 2019, Wang et al., 2018, Boltengagen et al., 2016, Dietz et al., 2015, Nikalayevich and Ohkura, 2015, Takahara and Takahashi, 2015, Zirin et al., 2015, Gonzalez et al., 2014, Moy et al., 2014, Kwon et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Korenjak et al., 2012, Malone et al., 2012, Friedman et al., 2011, Hartmann et al., 2011, Qurashi et al., 2011, Zimek and Weber, 2011, Kallappagoudar et al., 2010, Yamamoto-Hino et al., 2010, Christensen et al., 2009.5.6, Dworkin et al., 2009, Katzenberger et al., 2009, Nie et al., 2009, Fisher et al., 2008, Meignin and Davis, 2008, Goshima et al., 2007, Christensen and Cook, 2006.7.11, Gesellchen et al., 2005, Nybakken et al., 2005, Rehwinkel et al., 2004)
                UAP56
                (Huang and Wong, 2021, Cacchione et al., 2020, Gamez et al., 2020, Sato and Siomi, 2020, ElMaghraby et al., 2019, Hirakata and Siomi, 2019, Kneuss et al., 2019, Yamashiro and Siomi, 2018, Zhang et al., 2018, Huang et al., 2017, Pritykin et al., 2017, Specchia et al., 2017, Dennis et al., 2016, Hirakata and Siomi, 2016, Chen and Aravin, 2015, Hayashi et al., 2014, Czech et al., 2013, Guzzardo et al., 2013, Zhang et al., 2012, Ardehali et al., 2009, Katzenberger et al., 2009, Farny et al., 2008, Meignin and Davis, 2008, Hieronymus and Silver, 2004, Rehwinkel et al., 2004, Herold et al., 2003, Gatfield and Izaurralde, 2002)
                Uap56
                (Ho et al., 2024, Dehghani and Lasko, 2017, Katahira, 2012)
                WM6
                (Eisen et al., 1998, Warbrick, 1994.6.20, Warbrick and Glover, 1994)
                Wm6
                dHel25E
                (Goodman et al., 2019)
                hel
                (Wallrath, 1998, Eberl et al., 1997, Lorenz et al., 1995)
                hel25E
                (Rajasekaran et al., 2021)
                jf26
                (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
                l(2)25Eb
                l(2)gdh-12
                (Kotarski et al., 1983)
                l(2)gdh12
                l(2)jf26
                l(2)k11511
                (Meignin and Davis, 2008)
                sz
                (Meignin and Davis, 2008)
                uap56
                (Kneuss et al., 2019, Zhang et al., 2018, Abramov et al., 2016, Hur et al., 2016, Han et al., 2015, Iampietro et al., 2014, Zhang et al., 2014, Muerdter et al., 2013, Meignin and Davis, 2008, Meignin and Davis, 2005)
                Name Synonyms
                Hel25E
                (Ulvila et al., 2010)
                Helicase at 25E
                (Yamashiro and Siomi, 2018, Hirakata and Siomi, 2016, Nguyen et al., 2016)
                RNA helicase
                (Melnick, 1992.11.9)
                helicase
                (Lorenz et al., 1995)
                lethal(2)25Eb
                Secondary FlyBase IDs
                • FBgn0001929
                • FBgn0008648
                • FBgn0021910
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 61 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                References (173)