FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Hel25E, thoc5
FlyBase ID
FBig0000104466
Interaction Network
Interactions Browser links
Hel25E network
thoc5 network
Reported Interactions
FBrf0231583-1.coIP.WB
Reference
Hur et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
thoc5 

Thoc5

bait

FLAG tag

Hel25E 

UAP56

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The thoc5-Hel25E interaction is RNA-independent (not affected by RNase).

(Hur et al., 2016)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hur et al., 2016)
FBrf0240110-16.coIP.MS
Reference
Zhang et al., 2018
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
thoc5 

Thoc5

bait

Venus tag

Hel25E 

UAP56

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zhang et al., 2018)
FBrf0240110-19.coIP.MS
Reference
Zhang et al., 2018
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hel25E 

UAP56

bait

Venus tag

thoc5 

Thoc5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zhang et al., 2018)
FBrf0240110-4.coIP.MS
Reference
Zhang et al., 2018
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hel25E 

UAP56

bait

Venus tag

Hel25E 
thoc5 

Thoc5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hel25E 
interaction domain
mutation decreasing interaction
unspecified

Hel25E[sz15] allele

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zhang et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)