FlyBase Allele Report: Dmel\asf1[UAS.cMa]

General Information

Symbol

Dmel\asf1^UAS.cMa

Species

D. melanogaster

Name

FlyBase ID

FBal0141248

Feature type

allele

Associated gene

Dmel\asf1

Associated Insertion(s)

Carried in Construct

P{UAS-asf1.M}

Also Known As

UAS-asf1

Key Links

Transgenic product class

cDNA

(Moshkin et al., 2002)

wild_type

(Moshkin et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Moshkin et al., 2002)

wild_type

(Moshkin et al., 2002)

Mutagen

in vitro construct

(Moshkin et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-asf1.M}

Cytology

Description

UASt regulatory sequences drive expression of a full-length asf1 cDNA.

(Moshkin et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

asf1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage | heat sensitive, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

some die during larval stage | heat sensitive, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

visible, with Scer\GAL4^ey.PH

(Moshkin et al., 2009)

Phenotype Manifest In

embryonic/larval salivary gland, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

eye, with Scer\GAL4^ey.PH

(Moshkin et al., 2009, Carrera et al., 2003, Moshkin et al., 2002)

larval salivary gland & nucleus, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

macrochaeta, with Scer\GAL4^hs.2sev

(Carrera et al., 2003)

Detailed Description

Statement

Reference

Expression of asf1^Scer\UAS.cMa under the control of Scer\GAL4^ey.PH results in a small, rough eye phenotype.

(Moshkin et al., 2009)

Expression of asf1^Scer\UAS.cMa when driven by Scer\GAL4^ey.PH leads to a mild rough eye phenotype. Expression of asf1^Scer\UAS.cMa driven by a 2 hour heatshock (driven by Scer\GAL4^hs.2sev) at any time during larval development is lethal. When expressed at low enough levels for the animals to survive to adulthood, the only phenotype seen is greatly reduced larval salivary glands. Severe defects are seen in the morphology of the salivary gland nuclei, chromosomes are reduced in size. If these animals develop to adulthood a number of macrochaetae are seen to be missing, and the sex ratio of those adults is skewed towards females.

(Carrera et al., 2003)

Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa flies have a rough eye phenotype.

(Moshkin et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, asf1^UAS.cMa has visible phenotype, suppressible by lid[+]/Kdm5¹⁰⁴²⁴

(Moshkin et al., 2009)

Scer\GAL4^ey.PH, asf1^UAS.cMa has visible phenotype, suppressible by Kdm5^k06801/lid[+]

(Moshkin et al., 2009)

Scer\GAL4^ey.PH, asf1^UAS.cMa has visible phenotype, suppressible by Sin3A[+]/Sin3A⁰⁸²⁶⁹

(Moshkin et al., 2009)

Enhancer of

Statement

Reference

asf1^UAS.cMa/Scer\GAL4^ey.PH is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^hs.2sev, Tlk^UAS.cCa

(Carrera et al., 2003)

Suppressor of

Statement

Reference

asf1^UAS.cMa, Scer\GAL4^ey.PH is a suppressor of visible phenotype of Caf1-180^UAS.cSa, Scer\GAL4^ey.PH

(Song et al., 2007)

asf1^UAS.cMa, Scer\GAL4^ey.PH is a suppressor of increased cell death phenotype of Caf1-180^UAS.cSa, Scer\GAL4^ey.PH

(Song et al., 2007)

Other

Statement

Reference

Scer\GAL4^ey.PH, Tlk^UAS.cCa, asf1^UAS.cMa has abnormal size | adult stage phenotype

(Carrera et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, asf1^UAS.cMa has salivary gland & nucleus & larva phenotype, enhanceable by Tlk^UAS.cCa

(Carrera et al., 2003)

Scer\GAL4^hs.2sev, asf1^UAS.cMa has embryonic/larval salivary gland phenotype, enhanceable by Tlk^UAS.cCa

(Carrera et al., 2003)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, enhanceable by Tlk^UAS.cCa

(Carrera et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, suppressible by lid[+]/Kdm5¹⁰⁴²⁴

(Moshkin et al., 2009)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, suppressible by Kdm5^k06801/lid[+]

(Moshkin et al., 2009)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, suppressible by Sin3A[+]/Sin3A⁰⁸²⁶⁹

(Moshkin et al., 2009)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, suppressible by mor[+]/mor¹

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, suppressible by osa²/osa[+]

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, suppressible by brm¹/brm[+]

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, suppressible by brm[+]/brm²

(Moshkin et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, non-suppressible by Asx^XF23/Asx[+]

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, non-suppressible by Df(3R)red-P6/+

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, non-suppressible by E(z)[+]/E(z)⁶⁰

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, non-suppressible by Pc³/Pc[+]

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, non-suppressible by Pcl⁵/Pcl[+]

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, non-suppressible by Psc[+]/Psc¹

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, non-suppressible by kto[+]/kto¹

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, non-suppressible by ph-p[+]/ph-p⁴¹⁰

(Moshkin et al., 2002)

Scer\GAL4^ey.PH, asf1^UAS.cMa has eye phenotype, non-suppressible by tara^L4/tara[+]

(Moshkin et al., 2002)

Enhancer of

Statement

Reference

asf1^UAS.cMa is an enhancer of larval salivary gland & nuclear chromosome phenotype of Scer\GAL4^hs.2sev, Tlk^UAS.cCa

(Carrera et al., 2003)

asf1^UAS.cMa is an enhancer of embryonic/larval salivary gland phenotype of Scer\GAL4^hs.2sev, Tlk^UAS.cCa

(Carrera et al., 2003)

asf1^UAS.cMa is an enhancer of eye phenotype of Scer\GAL4^ey.PH, Tlk^UAS.cCa

(Carrera et al., 2003)

asf1^UAS.cMa is an enhancer of eye phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Moshkin et al., 2002)

Suppressor of

Statement

Reference

asf1^UAS.cMa, Scer\GAL4^ey.PH is a suppressor of eye phenotype of Caf1-180^UAS.cSa, Scer\GAL4^ey.PH

(Song et al., 2007)

asf1^UAS.cMa is a suppressor of head phenotype of Scer\GAL4^ey.PH, Tlk^dead.UAS

(Carrera et al., 2003)

asf1^UAS.cMa is a suppressor of eye phenotype of Scer\GAL4^ey.PH, Tlk^dead.UAS

(Carrera et al., 2003)

Other

Statement

Reference

Scer\GAL4^ey.PH, Tlk^UAS.cCa, asf1^UAS.cMa has head phenotype

(Carrera et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The small, rough eye phenotype caused by expression of asf1^Scer\UAS.cMa under the control of Scer\GAL4^ey.PH is significantly suppressed by lid¹⁰⁴²⁴/+, lid^k06801/+ or Sin3A⁰⁸²⁶⁹/+.

(Moshkin et al., 2009)

The small-eye phenotype is suppressed in flies co-expressing asf1^Scer\UAS.cMa with Caf1-180^Scer\UAS.cSa under the control of Scer\GAL4^ey.PH.

(Song et al., 2007)

Co-expression of tlk^Scer\UAS.cCa and asf1^Scer\UAS.cMa driven by Scer\GAL4^ey.PH leads to a massive reduction in head and eye structures, creating a 'pin-head' phenotype. The pin-head phenotype seen in tlk^{dead.Scer\UAS}, Scer\GAL4^ey.PH mutants is significantly suppressed by co-expression of asf1^Scer\UAS.cMa. When tlk^Scer\UAS.cCa and asf1^Scer\UAS.cMa are driven by Scer\GAL4^hs.2sev in conditions that allow animals to survive to adulthood, the salivary gland defects are enhanced.

(Carrera et al., 2003)

Df(3R)red-P6/+ does not suppress the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. kto¹/+ does not suppress the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. tara^L4/+ does not suppress the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. Asx^XF23/+ does not suppress the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. ph-p⁴¹⁰/+ does not suppress the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. Pc³/+ does not suppress the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. Pcl⁵/+ does not suppress the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. Psc¹/+ does not suppress the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. E(z)⁶⁰/+ does not suppress the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. brm¹/+ suppresses the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. brm²/+ suppresses the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. mor¹/+ suppresses the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa. osa²/+ suppresses the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa.

(Moshkin et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

asf1^Scer\UAS.cMa

asf1^UAS.cMa

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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