mesothoracic leg & sex comb | ectopic
ph-p410 homozygous adult males do not show ectopic sex combs on the prothoracic leg.
ph-p410/Y males display a homeotic transformation of the mesothoracic legs into prothoracic legs manifesting as extra sex combs.
Mutants show a low number of ectopic sex combs on the second leg.
90% of ph-p410/Y flies have ectopic sex combs on the second leg, but they do not have ectopic sex combs on the third leg.
ph-p410/+ females show weak suppression of the telomeric position effect (TPE) of P{hsp26-pt-T}39C-5, while ph-p410 homozygous females and hemizygous males show a strong suppression of TPE. ph-p410/+ suppresses the telomeric position effect (TPE) of P{hsp26-pt-T}39C-27, P{hsp26-pt-T}39C-31 and P{wA}4-4.
Heterozygous males have ectopic sex comb teeth on the T2 (penetrance 38%) and T3 (penetrance 24%) legs.
Heterozygous animals have a mild transformation of the fourth abdominal segment into the fifth - patches of pigmentation are seen in the anterior part of A4.
More than 90% of ph-p410 males have extra sex comb teeth on the metathoracic legs.
Transformation of second to first leg. Transformation of third to second leg. Transformation of abdominal segment A6 to A7.
Hemizygous males usually have sex combs on the second and third legs and show a partial transformation of abdominal segment A4 towards A5, at both 25 and 29oC. Hemizygous males occasionally show a partial transformation of the posterior wing compartment towards the haltere at 25oC. The penetrance of this phenotype increases at 29oC.
Phenoytpe manifest in: metathoracic leg Shows a metathoracic leg to prothoracic leg transformation.
Adults viable.
ph-p410 has visible | recessive phenotype, enhanceable by lolalk02512
ph-p410 has visible | homeotic phenotype, enhanceable by Eip75B333.15
ph-p410 has visible phenotype, enhanceable by e(Pc)84DET66.1/e(Pc)84DET66.1
Pc3, ph-p[+]/ph-p410 has visible | dominant phenotype, enhanceable by E(Pc)17B[+]/E(Pc)17B2.2.31
Pc3/Pc[+], ph-p410 has visible | dominant phenotype, enhanceable by E(Pc)17B[+]/E(Pc)17B2.2.31
ph-p410 has homeotic phenotype, suppressible by Marcal1[+]/Marcal1del
ph-p410 has homeotic phenotype, suppressible by Marcal1del/Marcal1del
Pc1, ph-p[+]/ph-p410 has homeotic phenotype, suppressible | partially by Marcal1del/Marcal1del
ph-p410 has visible | recessive | homeotic phenotype, suppressible by lolalUAS.EGFP/Scer\GAL4da.G32
ph-p410 has visible | recessive | homeotic phenotype, suppressible by Scer\GAL4da.G32/lolalUAS.Tag:FLAG
ph-p410 has visible | recessive | homeotic phenotype, suppressible by lolalk07907
ph-p410 has visible | recessive | homeotic phenotype, suppressible by lolalk11212
ph-p410 has visible | recessive phenotype, suppressible by Df(2R)M60E/+
ph-p410 has visible | recessive phenotype, suppressible by Df(3R)sbd45/+
ph-p410 has visible | recessive phenotype, suppressible by M(2)58F[+]/M(2)58Funspecified
ph-p410 has visible | recessive phenotype, suppressible by M(2)56Funspecified/M(2)56F[+]
Pc3/Pc[+], ph-p410 has visible | dominant phenotype, suppressible by Su(Pc)35CDT41.2/Su(Pc)35CD[+]
Pc3, ph-p[+]/ph-p410 has visible | dominant phenotype, suppressible by Su(Pc)35CDT41.2/Su(Pc)35CD[+]
ph-p410 has visible phenotype, suppressible by Su(Pc)35CDT41.2/Su(Pc)35CD[+]
ph-p410 is an enhancer of visible | male phenotype of dacΔPRE2.ΔPRE1
ph-p410 is an enhancer of visible | male phenotype of dacPRE2.GAGAmut
ph-p[+]/ph-p410 is a suppressor of visible | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU
ph-p[+]/ph-p410 is a suppressor of visible | adult stage phenotype of Hsap\SMARCAL1UAS.cBa, Scer\GAL4Bx-MS1096
ph-p410 is a suppressor | partially of abnormal eye color phenotype of wph-p.lac+3/w1118
ph-p410 is a suppressor of abnormal eye color phenotype of wT81
en79.Tag:HA, ph-p410 has abnormal body color phenotype
Asx3, P{hsp26-pt-T}39C-5, ph-p410 has suppressor of variegation phenotype
Pc1, P{hsp26-pt-T}39C-5, ph-p410 has suppressor of variegation phenotype
Pc6, P{hsp26-pt-T}39C-5, ph-p410 has suppressor of variegation phenotype
P{hsp26-pt-T}39C-5, ScmET50, ph-p410 has suppressor of variegation phenotype
AsxXF23, P{hsp26-pt-T}39C-5, ph-p410 has suppressor of variegation phenotype
Hsc70-403550, ph-p410 has viable phenotype
Hsc70-4195, ph-p410 has viable phenotype
Hsc70-4[+]/Hsc70-4I-R35-R7, ph-p410 has lethal phenotype
Hsc70-4II-R35-R2/Hsc70-4[+], ph-p410 has lethal phenotype
Hsc70-4II-R35-R7/Hsc70-4[+], ph-p410 has lethal phenotype
Hsc70-4+t14/Hsc70-4[+], ph-p410 has viable phenotype
Hsc70-403550/Hsc70-4[+], ph-p410 has viable phenotype
Hsc70-4195/Hsc70-4[+], ph-p410 has viable phenotype
Hsc70-4PB2/Hsc70-4[+], ph-p410 has viable phenotype
Hsc70-4[+]/Hsc70-454.1, ph-p410 has lethal | pharate adult stage phenotype
Hsc70-454.1, ph-p410 has lethal | pharate adult stage phenotype
Hsc70-4I-R35-R7, ph-p410 has lethal phenotype
Hsc70-4PB2, ph-p410 has viable phenotype
Hsc70-4II-R35-R7, ph-p410 has lethal phenotype
Hsc70-4II-R35-R2, ph-p410 has lethal phenotype
Hsc70-4+t14, ph-p410 has viable phenotype
Df(2R)en-SFX31, ph-p410 has visible phenotype
ph-p410 has mesothoracic leg phenotype, enhanceable by lolalk02512
ph-p410 has metathoracic leg phenotype, enhanceable by lolalk02512
ph-p410 has prothoracic leg | ectopic phenotype, enhanceable by lolalk02512
ph-p410 has sex comb tooth phenotype, enhanceable by lolalk02512
ph-p410 has larval abdominal segment 5 | ectopic phenotype, enhanceable by chm14
ph-p410 has larval abdominal segment 4 phenotype, enhanceable by chm14
ph-p410 has sex comb phenotype, enhanceable by Eip75B333.15
ph-p410 has metathoracic leg phenotype, enhanceable by effmer1/eff[+]
ph-p410 has metathoracic leg phenotype, enhanceable by eff[+]/effmer4
ph-p410 has sex comb | ectopic phenotype, enhanceable by Hsc70-4[+]/Hsc70-4I-R35-R7
ph-p410 has sex comb | ectopic phenotype, enhanceable by Hsc70-4II-R35-R2/Hsc70-4[+]
ph-p410 has sex comb | ectopic phenotype, enhanceable by Hsc70-4II-R35-R7/Hsc70-4[+]
ph-p410 has sex comb | ectopic phenotype, enhanceable by Hsc70-403550/Hsc70-4[+]
ph-p410 has sex comb | ectopic phenotype, enhanceable by Hsc70-4[+]/Hsc70-454.1
ph-p410 has abdominal segment 6 phenotype, enhanceable by dom14/dom[+]
ph-p410 has mesothoracic leg phenotype, enhanceable by dom14/dom[+]
ph-p410 has metathoracic leg phenotype, enhanceable by dom14/dom[+]
ph-p410 has metathoracic leg phenotype, enhanceable by e(Pc)84DET66.1/e(Pc)84DET66.1
Pc3, ph-p[+]/ph-p410 has antenna phenotype, enhanceable by E(Pc)17B[+]/E(Pc)17B2.2.31
Pc3/Pc[+], ph-p410 has antenna phenotype, enhanceable by E(Pc)17B[+]/E(Pc)17B2.2.31
ph-p410 has sex comb | ectopic phenotype, non-enhanceable by Df(3R)BSC509/+
ph-p410 has sex comb | ectopic phenotype, non-enhanceable by Df(3R)Exel6178/+
ph-p410 has sex comb | ectopic phenotype, non-enhanceable by Df(3R)wrd-CG7208/+
ph-p410 has sex comb | ectopic phenotype, non-enhanceable by +/Df(3R)ED6265
ph-p410 has sex comb | ectopic phenotype, non-enhanceable by Df(3R)ED5474/+
ph-p410 has sex comb | ectopic phenotype, non-enhanceable by Df(3R)ED6025/+
ph-p410 has sex comb | ectopic phenotype, non-enhanceable by +/Df(2L)ED12527
ph-p410 has sex comb | ectopic phenotype, non-enhanceable by Hsc70-4+t14/Hsc70-4[+]
ph-p410 has sex comb | ectopic phenotype, non-enhanceable by Hsc70-4PB2/Hsc70-4[+]
ph-p410 has sex comb phenotype, suppressible by Marcal1[+]/Marcal1del
ph-p410 has sex comb phenotype, suppressible by Marcal1del/Marcal1del
Pc1, ph-p[+]/ph-p410 has wing phenotype, suppressible | partially by Marcal1del/Marcal1del
ph-p410 has prothoracic leg | ectopic phenotype, suppressible by lolalUAS.EGFP/Scer\GAL4da.G32
ph-p410 has prothoracic leg | ectopic phenotype, suppressible by Scer\GAL4da.G32/lolalUAS.Tag:FLAG
ph-p410 has prothoracic leg | ectopic phenotype, suppressible by lolalk07907
ph-p410 has prothoracic leg | ectopic phenotype, suppressible by lolalk11212
ph-p410 has sex comb tooth phenotype, suppressible by lolal+tBB
ph-p410 has sex comb tooth phenotype, suppressible by lolalUAS.EGFP/Scer\GAL4da.G32
ph-p410 has sex comb tooth phenotype, suppressible by Scer\GAL4da.G32/lolalUAS.Tag:FLAG
ph-p410 has mesothoracic leg phenotype, suppressible by lolal+tBB
ph-p410 has mesothoracic leg phenotype, suppressible by lolal311a
ph-p410 has mesothoracic leg phenotype, suppressible by lolalUAS.EGFP/Scer\GAL4da.G32
ph-p410 has mesothoracic leg phenotype, suppressible by Scer\GAL4da.G32/lolalUAS.Tag:FLAG
ph-p410 has mesothoracic leg phenotype, suppressible by lolalk07907
ph-p410 has mesothoracic leg phenotype, suppressible by lolalk11212
ph-p410 has metathoracic leg phenotype, suppressible by lolal+tBB
ph-p410 has metathoracic leg phenotype, suppressible by lolal311a
ph-p410 has metathoracic leg phenotype, suppressible by lolalUAS.EGFP/Scer\GAL4da.G32
ph-p410 has metathoracic leg phenotype, suppressible by Scer\GAL4da.G32/lolalUAS.Tag:FLAG
ph-p410 has metathoracic leg phenotype, suppressible by lolalk07907
ph-p410 has metathoracic leg phenotype, suppressible by lolalk11212
ph-p410 has prothoracic leg | ectopic phenotype, suppressible by lolal+tBB
ph-p410 has prothoracic leg | ectopic phenotype, suppressible by lolal311a
ph-p410 has mesothoracic leg phenotype, suppressible by tara1
ph-p410 has mesothoracic leg phenotype, suppressible by taraL4
ph-p410 has metathoracic leg phenotype, suppressible by tara1
ph-p410 has metathoracic leg phenotype, suppressible by taraL4
ph-p410 has metathoracic leg phenotype, suppressible by Df(2L)al/+
ph-p410 has metathoracic leg phenotype, suppressible by Df(2R)M60E/+
ph-p410 has metathoracic leg phenotype, suppressible by Df(3R)sbd45/+
ph-p410 has metathoracic leg phenotype, suppressible by M(2)56Funspecified/M(2)56F[+]
ph-p410 has metathoracic leg phenotype, suppressible by M(2)58F[+]/M(2)58Funspecified
ph-p410 has metathoracic leg phenotype, suppressible by RpLP1[+]/RpLP1beo
ph-p410 has metathoracic leg phenotype, suppressible by tara1/tara[+]
ph-p410 has metathoracic leg phenotype, suppressible by tou[+]/tou1
ph-p410 has metathoracic leg phenotype, suppressible by tou2/tou[+]
ph-p410 has metathoracic leg phenotype, suppressible by trx[+]/trxlac1
ph-p410 has metathoracic leg phenotype, suppressible by trx[+]/trxlac2
ph-p410 has sex comb | ectopic phenotype, suppressible by Df(2R)M60E/+
ph-p410 has sex comb | ectopic phenotype, suppressible by Df(3R)sbd45/+
ph-p410 has sex comb | ectopic phenotype, suppressible by M(2)56Funspecified/M(2)56F[+]
ph-p410 has sex comb | ectopic phenotype, suppressible by M(2)58F[+]/M(2)58Funspecified
ph-p410 has sex comb | ectopic phenotype, suppressible by Hsc70-4195/Hsc70-4[+]
ph-p410 has mesothoracic leg phenotype, suppressible by Df(3R)P14
ph-p410 has metathoracic leg phenotype, suppressible by Df(3R)DG4
ph-p410 has metathoracic leg phenotype, suppressible by Df(3R)P14
ph-p410 has mesothoracic leg phenotype, suppressible by Df(3R)DG4
Pc3/Pc[+], ph-p410 has antenna phenotype, suppressible by Su(Pc)35CDT41.2/Su(Pc)35CD[+]
Pc3, ph-p[+]/ph-p410 has antenna phenotype, suppressible by Su(Pc)35CDT41.2/Su(Pc)35CD[+]
ph-p410 has metathoracic leg phenotype, suppressible by Su(Pc)35CDT41.2/Su(Pc)35CD[+]
ph-p410 has sex comb | ectopic phenotype, non-suppressible by +/Df(3R)ED6265
ph-p410 has sex comb | ectopic phenotype, non-suppressible by Df(3R)ED5474/+
ph-p410 has sex comb | ectopic phenotype, non-suppressible by Df(3R)ED6025/+
ph-p410 has sex comb | ectopic phenotype, non-suppressible by +/Df(2L)ED12527
ph-p410 has sex comb | ectopic phenotype, non-suppressible by Df(3R)BSC509/+
ph-p410 has sex comb | ectopic phenotype, non-suppressible by Df(3R)Exel6178/+
ph-p410 has sex comb | ectopic phenotype, non-suppressible by Df(3R)wrd-CG7208/+
ph-p410 has mesothoracic leg phenotype, non-suppressible by Df(3R)gl-BX3
ph-p410 has metathoracic leg phenotype, non-suppressible by Df(3R)gl-BX3
ph-p410 has sex comb | ectopic phenotype, non-suppressible by Df(3R)gl-BX3
ph-p410 is an enhancer of prothoracic tarsal segment 2 | male phenotype of dacΔPRE2
ph-p410 is an enhancer of sex comb | ectopic phenotype of dacΔPRE2.ΔPRE1
ph-p410 is an enhancer of prothoracic tarsal segment 2 | male phenotype of dacΔPRE2.ΔPRE1
ph-p410 is an enhancer of sex comb | ectopic phenotype of dacPRE2.GAGAmut
ph-p410 is an enhancer of prothoracic tarsal segment 2 | male phenotype of dacPRE2.GAGAmut
ph-p[+]/ph-p410 is a suppressor of wing vein | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU
ph-p[+]/ph-p410 is a suppressor of wing vein | adult stage phenotype of Hsap\SMARCAL1UAS.cBa, Scer\GAL4Bx-MS1096
ph-p[+]/ph-p410 is a suppressor of adult mesothoracic segment | ectopic phenotype of E(z)Trm
ph-p[+]/ph-p410 is a suppressor of adult metathoracic segment phenotype of E(z)Trm
ph-p[+]/ph-p410 is a suppressor of adult prothoracic segment phenotype of E(z)Trm
ph-p[+]/ph-p410 is a non-suppressor of eye phenotype of Scer\GAL4ey.PH, asf1UAS.cMa
en79.Tag:HA, ph-p410 has microchaeta | adult stage phenotype
dacΔPRE1, ph-p410 has prothoracic tarsal segment 2 | male phenotype
dacgypsyI, ph-p410 has prothoracic tarsal segment 2 | male | heat sensitive phenotype
lolalk02512, ph-p410 has sex comb | ectopic phenotype
lolalk02512, ph-p410 has abdominal segment 4 phenotype
lolalk02512, ph-p410 has abdominal segment 5 phenotype
lolalk02512, ph-p410 has abdominal segment 6 phenotype
lolalk02512, ph-p410 has abdominal segment 5 | ectopic phenotype
lolalk02512, ph-p410 has abdominal segment 6 | ectopic phenotype
lolalk02512, ph-p410 has abdominal segment 7 | ectopic phenotype
lolalk02512/lolal[+], ph-p410 has sex comb | ectopic phenotype
lolalk02512/lolal[+], ph-p410 has abdominal segment 4 phenotype
lolalk02512/lolal[+], ph-p410 has abdominal segment 5 phenotype
lolalk02512/lolal[+], ph-p410 has abdominal segment 6 phenotype
lolalk02512/lolal[+], ph-p410 has abdominal segment 5 | ectopic phenotype
lolalk02512/lolal[+], ph-p410 has abdominal segment 6 | ectopic phenotype
lolalk02512/lolal[+], ph-p410 has abdominal segment 7 | ectopic phenotype
Hsc70-454.1, ph-p410 has arista phenotype
Hsc70-454.1, ph-p410 has mesothoracic leg phenotype
Hsc70-4[+]/Hsc70-454.1, ph-p410 has arista phenotype
Hsc70-4[+]/Hsc70-454.1, ph-p410 has mesothoracic leg phenotype
Hsc70-4[+]/Hsc70-454.1, ph-p410 has metathoracic leg phenotype
Hsc70-454.1, ph-p410 has metathoracic leg phenotype
Hsc70-4[+]/Hsc70-454.1, ph-p410 has adult thoracic sensillum phenotype
Hsc70-4[+]/Hsc70-454.1, ph-p410 has abdominal tergite phenotype
Hsc70-4[+]/Hsc70-454.1, ph-p410 has abdominal sternite phenotype
Hsc70-454.1, ph-p410 has adult thoracic sensillum phenotype
Hsc70-454.1, ph-p410 has abdominal tergite phenotype
Hsc70-454.1, ph-p410 has abdominal sternite phenotype
en4, ph-p410 has wing vein L4 phenotype
en54, ph-p410 has wing vein L4 phenotype
en54, hhhs.PI, ph-p410 has wing vein L4 phenotype
en54, hhts2, ph-p410 has wing vein L4 phenotype
enC, ph-p410 has wing vein L4 phenotype
enXho25, ph-p410 has wing vein L4 phenotype
Df(2R)en-SFX31, ph-p410 has wing vein L4 phenotype
en79.Tag:HA/+, ph-p410/Y males show severely disrupted pigmentation and bristle pattern of the abdomen; en79.Tag:HA/+, ph-p410/+ females do not show this defects.
The ectopic sex comb phenotype observed in dacΔPRE2, dacΔPRE2 or dacPRE2.GAGAmut homozygotes is enhanced to full/near full penetrance by ph-p410; a nearly fully penetrant phenotype is also observed when dacΔPRE2.ΔPRE1 is in heterozygosity.
dacΔPRE1, ph-p410 double mutant adult males frequently show an ectopic sex comb on the T2 prothoracic leg segment, which is not observed in dacΔPRE1 single mutants or wild-type controls; the phenotype progresses to almost full penetrance from 21[o]C to 25[o]C.
dacgypsyI, ph-p410 double mutant adult males rarely show an ectopic sex comb on the T2 prothoracic leg segment only at 28[o]C, and never at 21[o]C or 25[o]C.
The formation of ectopic wing veins observed in adult flies expressing Marcal1Scer\UAS.cBa under the control of Scer\GAL4tub.PU is ameliorated by combination with a single copy of ph-p410.
The homeotic transformation of the mesothoracic legs into prothoracic legs manifesting as extra sex combs characteristic for ph-p410/Y males is partially suppressed by Marcal1del heterozygosity and to a greater extent (yet still not a full rescue) by Marcal1del homozygosity.
Similarly, the partial homeotic wing-to-haltere transformation observed in ph-p410/+;Pc1/+ double heterozygotes is ameliorated by combination with Marcal1del in homozygosity.
lolalk02512 enhances the ph-p410 esc-like phenotype, consisting of homeotic transformation of mesothoracic or metathoracic legs towards prothoracic legs due to the deregulation of Scr. In addition there is an increase in the mean number of sex comb teeth in ph-p410, lolalk02512 double mutants. One extra copy of lolal+tBB, lolalScer\UAS.T:Zzzz\FLAG or lolalScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32, suppresses the ph-p410 esc-like phenotype. Mutants heterozygous for lolalk02512, lolalk11212, or lolalk07907, when also heterozygous for ph-p410 exhibit a contrabithorax phenotype, which is a wing-to-haltere transformation due to ectopic expression of Ubx in the posterior part of the wing disc, often resulting in curvature of the wing. This phenotype is further enhanced in specific lolal heteroallelic viable combinations, such as lolalk02512/lolal311a, lolalk11212/lolal311a, and lolalk07907/lolal311a, when also heterozygous for ph-p410.
The addition of lolalk02512/+ to homozygous ph-p410 animals shows a partial homeotic transformation of A4 to A5, A5 to A6, and A6 to A7. There is a strong reduction of the size of the 6th tergite. Sex combs are seen on the second and third legs.
ph-p410/+ does not suppress the rough eye phenotype due to Scer\GAL4ey.PH; asf1Scer\UAS.cMa.
ph-p410 Hsc70-454.1/+ males die as pharate adults with complete ectopic sex combs on the second and third thoracic legs. These animals show malformed or missing abdominal tergites and sternites, a reduced number of thoracic bristles, and missing aristae.
Mutant heterozygotes show a strong suppression of the eye phenotype see in P{UAS-lacZ.Abd-B.5F24} leading to darker eyes.
100% of ph-p410/Y; en54/+, ph-p410/Y; en4/+, ph-p410/Y; enC/+ or ph-p410/Y; enXho25/+ double heterozygotes have a gap in the fourth wing vein at 25oC. 80% of ph-p410/Y; Df(2R)en-SFX31/+ double heterozygotes have a gap in the fourth wing vein at 25oC. ph-p410/Y; en54/+ is lethal at 29oC. 0% and 5% of ph-p410/+; en54/+ double heterozygotes have a gap in the fourth wing vein at 25oC and 29oC respectively. The penetrance of the phenotype at 29oC is increased to 25% if the flies are also expressing hhhs.PI and decreased to 0% if the flies are also heterozygous for hhts2.
Strong suppressor of eye pigment phenotype of wT81.
In combination with Pc3 (both heterozygous with wild type) an antenna to leg transformation is sometimes observed.
The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1Scer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 is ameliorated by combination with a single copy of ph-p410.
ph-dbr/ph-p410 does not show significant suppression of the nos mutant phenotype.