FlyBase Allele Report: Dmel\wrd[Δ]

General Information

Symbol

Dmel\wrd^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0258463

Feature type

allele

Associated gene

Dmel\wrd

Associated Insertion(s)

PBac{RB5.WH5}wrd^Δ

Carried in Construct

Also Known As

PP2A-B'^Δ

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Moazzen et al., 2009)

Progenitor genotype

PBac{RB}wrd^e03725

(Moazzen et al., 2009)

PBac{WH}wrd^f05278

(Moazzen et al., 2009)

Associated Insertion(s)

PBac{RB5.WH5}wrd^Δ

Cytology

Description

Generated as a FLP-mediated, site-specific recombination product between PBac{WH}PP2A-B'^f05278 and PBac{RB}PP2A-B'^e03725. Deletes all exons that encode the conserved protein domains of PP2A-B'. The only coding region remaining is a 3' exon of the alternatively spliced transcript PP2A-B'-RK.

(Moazzen et al., 2009)

Allele components

Component

Use(s)

Inserted element

PBac{RB5.WH5}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

complex_substitution

3R:18,169,400..18,177,187 [+]

Comment:

Deletion resulting from site-specific recombination between PBac{WH}PP2A-B'^f05278 and PBac{RB}PP2A-B'^e03725. A hybrid WH-RB element remains at the site.

(Moazzen et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile

(Moazzen et al., 2009)

male fertile

(Moazzen et al., 2009)

viable

(Moazzen et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Abnormally small autophagic structures are formed after starvation in mutant animals.

(Bánréti et al., 2012)

Mutants show no significant decrease in sex comb bristle number, the number of pseudotracheal rows, or the larval salivary glands.

(Moazzen et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

wrd^Δ/Df(3R)189, Scer\GAL4^{VP16.mat.αTub67C}, wdb^HMS01864 has female semi-sterile phenotype

(Jang et al., 2021)

wrd^Δ/Df(3R)189, Scer\GAL4^{VP16.mat.αTub67C}, wdb^HMS01864 has abnormal meiotic cell cycle | oogenesis phenotype

(Jang et al., 2021)

Ndc80^GL00625, Scer\GAL4^{VP16.mat.αTub67C}, wdb^HMS01864, wrd^Δ has abnormal meiotic cell cycle | oogenesis phenotype

(Jang et al., 2021)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

PP2A-B'[+]/wrd^Δ is a non-enhancer of sex comb | ectopic phenotype of Scr^Msc

(Moazzen et al., 2009)

PP2A-B'[+]/wrd^Δ is a non-enhancer of sex comb | ectopic phenotype of ph-p⁴¹⁰

(Moazzen et al., 2009)

NOT Suppressor of

Statement

Reference

PP2A-B'[+]/wrd^Δ is a non-suppressor of sex comb | ectopic phenotype of Scr^Msc

(Moazzen et al., 2009)

PP2A-B'[+], wrd^Δ, +, Df(3R)ED6265 is a non-suppressor of sex comb | ectopic phenotype of Scr^Msc

(Moazzen et al., 2009)

PP2A-B'[+], wrd^Δ, Df(3R)ED5474, + is a non-suppressor of sex comb | ectopic phenotype of Scr^Msc

(Moazzen et al., 2009)

PP2A-B'[+]/wrd^Δ is a non-suppressor of sex comb | ectopic phenotype of ph-p⁴¹⁰

(Moazzen et al., 2009)

Other

Statement

Reference

wrd^Δ/Df(3R)189, Scer\GAL4^{VP16.mat.αTub67C}, wdb^HMS01864 has oocyte phenotype

(Jang et al., 2021)

+/Df(3R)ED6265, wrd^Δ has sex comb phenotype

(Moazzen et al., 2009)

Df(3R)ED5474/+, wrd^Δ has sex comb phenotype

(Moazzen et al., 2009)

Df(3R)ED5474, Df(3R)ED6265/+, wrd^Δ has sex comb phenotype

(Moazzen et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Compared to PP2A-B'^Δ homozygotes, there is a significant increase in the number of sex comb bristles in flies that are homozygous for PP2A-B'^Δ and heterozygous for Df(3R)ED6265 (which uncovers wdb), Df(3R)ED5474 (which uncovers tws), or both these deletions. However, there is no increase in the number of pseudotracheal rows in the double mutants compared to PP2A-B'^Δ homozygotes.

Scr^Msc flies that are also heterozygous for PP2A-B'^Δ do not have fewer sex combs on the second leg than Scr^Msc mutants alone.

(Moazzen et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PP2A-B'^Δ

(Bánréti et al., 2012, Moazzen et al., 2009)

wrd^Δ

(Neal et al., 2022, Jang et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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