gypsy insulator sequence has been inserted into the genome, between the two Polycomb response elements (PREs) of the dac locus.
dacgypsyI homozygous adult males do not show ectopic sex combs on the prothoracic leg.
dacgypsyI, ph-p410 has visible | male | heat sensitive phenotype
dacgypsyI, ph-p410 has sex comb | ectopic | heat sensitive phenotype
dacgypsyI, ph-p410 has prothoracic tarsal segment 2 | male | heat sensitive phenotype
dacgypsyI, ph-p410 double mutant adult males rarely show an ectopic sex comb on the T2 prothoracic leg segment only at 28[o]C, and never at 21[o]C or 25[o]C.