FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\en4
Open Close
General Information
Symbol
Dmel\en4
Species
D. melanogaster
Name
FlyBase ID
FBal0003719
Feature type
allele
Associated gene
Dmel\en
Associated Insertion(s)
Carried in Construct
Also Known As
enLA4
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Karr et al., 1989, Kornberg, 1981)
Progenitor genotype
Cytology

Polytene chromosomes normal.

(Kornberg, 1981)
Description

Amino acid replacement: W422term.

(Gustavson et al., 1996)

Contains a translation stop at codon 422.

(Karr et al., 1989)

Point mutation causing truncation of the en gene product.

(Poole and Kornberg, 1988)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:11,526,405..11,526,405 [-]
Nucleotide change:

G11526405A

Reported nucleotide change:

G?A

Amino acid change:

W422term | en-PA; W422term | en-PB

Reported amino acid change:

W422term

Comment:

TGG to TGA

(Gustavson et al., 1996, Karr et al., 1989)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      A normal number of en and nub expressing md neurons are seen in mutants.

      (Brewster et al., 2001)

      The gnathal lobes are reduced in mutant embryos.

      (Rusch and Kaufman, 2000)

      Fat body is slightly reduced. Absence of demonstratable somatic gonadal precursor (SGP) cells.

      (Riechmann et al., 1998)

      Strong allele.

      (Gustavson et al., 1996)

      Transheterozygotes with Df(3L)E13 or Df(3R)slo8 produce flies with abnormal wings.

      (Saenz-Robles et al., 1995)

      Deviations from mitotic synchrony are seen in nuclear cycles 6-9 in en4 mutant embryos.

      (Karr et al., 1989)

      Third thoracic denticle belt and first abdominal fuse, as do denticle bands A2 and A3, A4 and A5, and A6 and A7. Mutant embryos show T3 fused to A1, A2 fused to A3, A4 fused to A5 and A6 fused to A7.

      (Poole and Kornberg, 1988)

      Defects in even naked cuticle.

      (Carroll and Scott, 1986)

      Male en4/en3 flies have abnormal genitalia, with a missing genital arch process and claspers, and reduced hypandrium, hypandrial phragma and penis apparatus. Extra genital arch and anal plate bristles are sometimes seen. Female en4/en3 flies generally have extra bristles and pigmentation on the dorsal part of the eighth tergite.

      (Epper and Sanchez, 1983)

      Homozygous clones in the anterior of the wing are normal, while homozygous clones in the posterior part of the wing are abnormal, producing socketed bristles characteristic of the anterior part of the wing. Homozygous embryos have abnormal denticle belts; the number of rows of denticles is reduced and they are disorganised. The denticle belts often appear fused.

      (Kornberg, 1981)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      en[+]/en4 is a suppressor of increased cell number | oogenesis phenotype of Scer\GAL4C587, Su(var)3-3NIG.17149R

      (Eliazer et al., 2014)
      Other
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      en4 has phenotype, suppressible by eRF1KY7/eRF1[+]

      (Chao et al., 2003)

      en4 has phenotype, suppressible by eRF1[+]/eRF1F2

      (Chao et al., 2003)
      Enhancer of
      Statement
      Reference

      en4 is an enhancer of phenotype of ci1

      (Locke and Hanna, 1996)

      en4 is an enhancer of phenotype of ci57

      (Locke and Hanna, 1996)

      en4 is an enhancer of phenotype of ci36

      (Locke and Hanna, 1996)
      NOT Enhancer of
      Statement
      Reference

      en[+]/en4 is a non-enhancer of wing vein | ectopic phenotype of Snr1E1

      (Marenda et al., 2004)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      en[+]/en4 is a non-suppressor of wing vein | ectopic phenotype of Snr1E1

      (Marenda et al., 2004)
      Other
      Statement
      Reference

      en4, ph-d2, ph-p2 has wing vein L4 phenotype

      (Maschat et al., 1998)

      en4, ph-p410 has wing vein L4 phenotype

      (Maschat et al., 1998)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      100% of ph-p410/Y; en4/+ double heterozygotes have a gap in the fourth wing vein at 25oC. 100% of ph-d2 ph-p2/Y ; en4/+ flies have a gap in the fourth wing vein at 29oC.

      (Maschat et al., 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      Kornberg.

      Comments
      Comments

      en mutants can be ranked by strength regarding their ability to induce a ci phenotype: en1 <= Df(2R)en30 <= en4 <= enEnci <= Df(2R)en-SFX31 <= en59.

      (Locke and Hanna, 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      References (19)