FlyBase Allele Report: Dmel\eRF1[KY7]

General Information

Symbol

Dmel\eRF1^KY7

Species

D. melanogaster

Name

FlyBase ID

FBal0147180

Feature type

allele

Associated gene

Dmel\eRF1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Chao et al., 2003)

Mutagen

ethyl methanesulfonate

(Bejsovec, 2003.6.3, Chao et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q162term.

(Chao et al., 2003)

Nucleotide substitution: C?T.

(Chao et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:20,350,780..20,350,780 [+]

Nucleotide change:

C20350780T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

Q162term

(Chao et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with eRF1⁰⁰¹⁰³)

(Chao et al., 2003)

lethal (with eRF1^neo28)

(Chao et al., 2003)

lethal - all die before end of larval stage | recessive

(Chao et al., 2003)

some die during larval stage | recessive

(Chao et al., 2003)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous embryos have no morphological defects and hatch normally. First instar larvae look normal but fail to grow and die after 7-8 days. Females carrying homozygous germline clones mated to males carrying either eRF1^KY7 or a deficiency for eRF1 do not produce eggs.

(Chao et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

eRF1^KY7/eRF1[+] is an enhancer of visible phenotype of Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

eRF1^KY7/eRF1[+] is an enhancer of eye phenotype of Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

Suppressor of

Statement

Reference

eRF1^KY7/eRF1[+] is a suppressor | maternal effect of phenotype of en⁷

(Chao et al., 2003)

eRF1^KY7/eRF1[+] is a suppressor of phenotype of en⁴

(Chao et al., 2003)

eRF1^KY7/eRF1[+] is a suppressor of ventral denticle belt | ectopic phenotype of wg^PE4

(Chao et al., 2003)

eRF1^KY7/eRF1[+] is a suppressor of embryonic/first instar larval cuticle phenotype of wg^PE4

(Chao et al., 2003)

NOT Suppressor of

Statement

Reference

eRF1^KY7/eRF1[+] is a non-suppressor of embryonic/first instar larval cuticle phenotype of wg^PE13

(Chao et al., 2003)

Other

Statement

Reference

Scer\GAL4^e22c, eRF1^KY7, wg^{PE4.UAS.cCa.Tag:HA} has ventral denticle belt phenotype

(Chao et al., 2003)

Scer\GAL4^e22c, eRF1^KY7, wg^{PE4.UAS.cCa.Tag:HA} has embryonic/first instar larval cuticle phenotype

(Chao et al., 2003)

Scer\GAL4^e22c, eRF1^KY7/eRF1[+], wg^{PE4.UAS.cCa.Tag:HA} has ventral denticle belt phenotype

(Chao et al., 2003)

Scer\GAL4^e22c, eRF1^KY7/eRF1[+], wg^{PE4.UAS.cCa.Tag:HA} has embryonic/first instar larval cuticle phenotype

(Chao et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Nonsense suppressor of the wg^PE4 mutation.

(Bejsovec, 2003.6.3)

The lawn of denticles phenotype seen in homozygous wg^PE4 embryos at 25^oC is suppressed by eRF1^KY7. This effect is a dominant maternal effect; wg^PE4 homozygous embryos laid by a female heterozygous for wg^PE4 and eRF1^KY7 show suppression of the wg^PE4 phenotype, whether or not the male parent carries eRF1^KY7. The wg^PE4 phenotype is suppressed more completely in wg^PE4 homozygous embryos derived from females carrying both eRF1^KY7 and Elf^LR16. The cuticle phenotype of wg^PE13 homozygous embryos is not suppressed if their female parent is heterozygous for eRF1^KY7. Embryos derived from females homozygous for wg^{PE4.Scer\UAS.cCa.T:Ivir\HA1} and heterozygous for eRF1^KY7 crossed to males homozygous for Scer\GAL4^e22c produce excess naked cuticle.