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General Information
Symbol
Dmel\w1118
Species
D. melanogaster
Name
FlyBase ID
FBal0018186
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
w1118
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Partial deletion of w.

Deletion of sequences including exon 1.

Partial deletion of w sequence, deletion is 5' to site of insertion of P-element in whd80k17.

The 5' half of w is deleted.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

21-days old w1118 mutant adults raised at 29[o]C do not show significantly changes in PPL1 neurons, as compared to age-matched controls.

Sexual arousal induced by the presence of a receptive virgin female is dramatically reduced in daylight in w1118 males compared to Oregon-R males (as measured by their courtship vigor index). The response of the w1118 males is considerably lower than that of blind (ninaB3) males.

The courtship vigor index of w1118 males to decapitated virgin females in daylight is three times lower than that of Oregon-R or blind (ninaB3) males.

In the dark, the courtship vigor index of w1118 and Oregon-R males towards a receptive virgin female is comparable.

In the dark, the courtship vigor index of w1118 and Oregon-R males towards a decapitated virgin female is comparable.

At low light intensities (11lx and 21lx) w1118 males court decapitated virgin females vigorously. At a low light intensity of 11lx w1118 males have a courtship vigor index towards receptive virgin females that is higher than under standard daylight conditions. The males are never seen to scan under these conditions.

In the dark, the courtship vigor index of w1118 males towards decapitated males is increased compared to that of Oregon-R males and is equal to the courtship vigor index of w1118 males towards decapitated females under these conditions.

Groups of naive w1118 males observed under daylight conditions do not court each other and thus do not show chaining behaviour, the same behaviour as is seen in Oregon-R males.

Eyes mutant for w1118 show retinal degeneration under constant light conditions.

Mutant flies have significantly fewer synaptic vesicles in the terminals of the photoreceptor cells R1-R6 in the lamina. However, in contrast to the absolute numbers of synaptic vesicles, the profile density is not significantly different from wild type. There is no significant difference in the normalized number of single-headed capitate projection profiles in the photoreceptor terminals, but the number of multiple-headed capitate projection profiles is higher than normal.

Mutant males display negligible intermale courtship upon recurring ethanol administration with 70% ethanol, in contrast to wild-type males which show intermale courtship after repeated ethanol exposure.

w1118 mutant flies exhibit memory performance levels that are approximately 60% of wild-type levels in a heat-box. w1118 mutants have approximately 30% of wild-type levels of serotonin and dopamine. Octopamine levels are approximately 80% of wild-type levels.

Mutants show a reduced decay of the initial locomotor activity in an open field arena compared to wild-type flies.

Eye colour: wph-p.lac+3/w1118 females have an orange eye colour.

Homozygotes have white eyes with only <1% red pigment (compared to 100% pigment in wild type).

Male w1118 flies have an increased resistance to the anesthetic enflurane compared to wild-type controls, as judged by climbing ability. This effect is true whether enflurane resistance is measured in regular light or red light. Male w1118 flies have an increased resistance to the anesthetic halothane compared to wild-type controls when climbing ability is measured in regular light. However, this resistance varies from only modest to marginal when climbing ability is measured in red light.

Not susceptible to p-Cresol.

Eye colour: white.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference

wT40/w1118 has eye color defective phenotype, suppressible | partially by Psc[+]/Psc1

wph-p.lac+3/w1118 has eye color defective phenotype, suppressible by Alh1/Alh[+]

w1118, wcad.PRE has eye color defective phenotype, suppressible by ph-p[+]/ph-p410

w1118, weya.PRE has eye color defective phenotype, suppressible by ph-p[+]/ph-p410

w1118, waPKC.PRE has eye color defective phenotype, suppressible by ph-p[+]/ph-p410

w1118, wprod.PRE has eye color defective phenotype, suppressible by ph-p[+]/ph-p410

Enhancer of
Statement
Reference

we(g)/w1118 is an enhancer of eye color defective phenotype of g50e

we(g)/w1118 is an enhancer of eye color defective phenotype of g53d

we(g)/w1118 is an enhancer of eye color defective phenotype of g2

Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

w1118 has retina | conditional phenotype, enhanceable by sdtUAS.D.Tag:FLAG/Scer\GAL4ninaE.PT

Scer\GAL4ninaE.PT, sdtUAS.D.Tag:FLAG, w1118 has retina | conditional phenotype, enhanceable by sdt[+]/sdtK85

NOT Enhanced by
Statement
Reference

w1118 has retina | conditional phenotype, non-enhanceable by sdt[+]/sdtK85

Suppressed by
Statement
Reference

w1118 has retina | conditional phenotype, suppressible by Scer\GAL4GMR.PF/sdtUAS.H.Tag:FLAG

sdt[+]/sdtK85, w1118 has retina | conditional phenotype, suppressible by Scer\GAL4GMR.PF/sdtUAS.H.Tag:FLAG

wph-p.lac+3/w1118 has pigment cell phenotype, suppressible by Alh1/Alh[+]

w1118, wcad.PRE has pigment cell phenotype, suppressible by ph-p[+]/ph-p410

w1118, weya.PRE has pigment cell phenotype, suppressible by ph-p[+]/ph-p410

w1118, waPKC.PRE has pigment cell phenotype, suppressible by ph-p[+]/ph-p410

w1118, wprod.PRE has pigment cell phenotype, suppressible by ph-p[+]/ph-p410

NOT suppressed by
Statement
Reference

w1118 has retina | conditional phenotype, non-suppressible by sdt[+]/sdtK85

Enhancer of
Statement
Reference

we(g)/w1118 is an enhancer of pigment cell phenotype of g53d

we(g)/w1118 is an enhancer of pigment cell phenotype of g50e

we(g)/w1118 is an enhancer of pigment cell phenotype of g2

Additional Comments
Genetic Interactions
Statement
Reference

In daylight, the courtship vigor index of w1118 ninaB3 double mutant males to both receptive virgin females and decapitated virgin females is comparable to that of ninaB3 single mutant males.

In the dark, the courtship vigor index of w1118 ninaB3 double mutant males to both receptive virgin females and decapitated virgin females is comparable to the response of Oregon-R males.

w1118; shi1.Scer\UAS flies establish more dominance relationships compared to w1118(CS); shi1.Scer\UAS pairs at both 19[o]C and 30[o]C.

The light dependent retinal degeneration seen in w1118 flies does not depend on sdtK85/+. The rate of photoreceptor cell survival seen in a w1118 genetic background is similar in sdtK85/+ or +/+ flies.

Expression of sdtScer\UAS.H.T:Zzzz\FLAG in outer photoreceptor cells under the control of Scer\GAL4ninaE.PT in sdtK85/+ or +/+ genetic background completely blocked the light induced retinal degeneration in w1118 flies. In addition, the rhabdomeres of the outer Scer\GAL4ninaE.PT-expressing photoreceptor cells are reduced in size in comparison with control flies and flies including flies kept in the dark.

Expression of sdtScer\UAS.D.T:Zzzz\FLAG under the control of Scer\GAL4ninaE.PT enhances the retinal degeneration phenotype in w1118 eyes. When sdtScer\UAS.D.T:Zzzz\FLAG is expressed in a sdtK85/+ background, the decrease in photoreceptor cell survival is stronger.

Eye colour: wph-p.lac+3/w1118 ; Alh1/+ females have a red eye colour.

Flies carrying one copy of either wcad.PRE, wprod.PRE, weya.PRE or waPKC.PRE in a w1118/w1118; ph-p410/+ double mutant background have darker eyes than flies with one copy of wcad.PRE, wprod.PRE, weya.PRE or waPKC.PRE in a single mutant w1118/w1118 background.

Flies with a w1118/we(g) mutant genotype enhance the eye colour defective phenotype of g2, g50e and g53d homozygous mutants leading to a greater reduction of red pigment in the eye. w1118, g50e double mutant flies do not show the increased male-male courtship behaviour that single g50e mutants exhibit.

nahar38, w1118 mutant flies show the same sensitivity to 0.1% enflurane or halothane when tested under red light as nahar38 mutant flies do. However, nahar38, w1118 mutant flies show a much higher sensitivity to 0.05% enflurane or halothane when tested under ambient light than nahar38 mutant flies do.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

w1118 is partially rescued by w+mC

Comments

The low courtship vigor index seen in daylight in w1118 males in response to a receptive virgin female is rescued by Dp(1;Y)w+y+.

w+mC rescues the low courtship vigor index seen in daylight in w1118 males in response to a receptive virgin female.

w1118 males rescued with either Dp(1;Y)w+y+ or w+mC court decapitated virgin females vigorously, independent of light conditions.

The reduced sexual discrimination of w1118 males in the dark (they show similar courtship levels towards decapitated males and decapitated females) is rescued by Dp(1;Y)w+y+, but is not rescued by w+mC.

In daylight, w1118 males rescued by Dp(1;Y)w+y+ show a clear courtship preference for decapitated females over decapitated males, with the level of courtship towards decapitated males under these conditions being comparable to that seen in Oregon-R males.

In daylight, w1118 males rescued by w+mC show a clear courtship preference for decapitated females over decapitated males, although the level of courtship towards decapitated males shown by the rescued males is higher than the level seen in Oregon-R males under these conditions.

Groups of naive w1118 males rescued by Dp(1;Y)w+y+ do not show chaining behaviour under daylight conditions, the same behaviour as is seen in Oregon-R males.

Groups of naive w1118 males rescued by w+mC show chaining behaviour under daylight conditions. This behaviour can be rescued by Dp(1;Y)w+y+.

Expression of wRESC.Scer\UAS restores eye color in w1118 mutants.

One copy of either w+mC or wHSBJ restores courtship disinhibition after repeated ethanol exposure (observed as intermale courtship) in w1118 males.

Images (0)
Mutant
Wild-type
Stocks (50,056)
Notes on Origin
Discoverer

R. Levis.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (55)