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Citation
Hazelrigg, T., Levis, R., Rubin, G.M. (1984). Transformation of white locus DNA in Drosophila: Dosage compensation, zeste interaction, and position effects.  Cell 36(): 469--481.
FlyBase ID
FBrf0040489
Publication Type
Research paper
Abstract

P-element-mediated DNA transformation was used to generate transformants carrying segments of DNA from the white locus of D. melanogaster. The vast majority of transduced copies of an 11.7 or a 14.3 kb segment of DNA from white successfully rescued the white- eye-color phenotype when inserted in many different chromosomal locations. However, two transformants with abnormal eye pigmentation--apparently a consequence of the genomic positions of the transduced white gene--were also recovered. In all seven cases tested, autosomal insertions of white, which is dosage-compensated in its normal location on the X chromosome, retained the property of dosage compensation. In contrast to the relative insensitivity of eye-color pigmentation and dosage compensation to genomic position, the transduced white DNA segments differed widely in their interactions with the zeste mutation, ranging from greater than normal repression by zeste to insensitivity to the presence of zeste.

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Alleles (16)
    Genes (4)
    Molecular Constructs (4)
    Molecular Segments (8)
    Natural transposons (1)
    Insertions (12)
    Transgenic Constructs (4)