Asx3 embryos from heterozygous mothers show segregation defects in anaphase and telophase as judged by the presence of chromatin bridges. Most of these chromatin bridges are probably resolved because large irregularly-shaped nuclei and polyploid nuclei are only seen at a low penetrance. Asx3 embryos also show a higher level of "nuclear fallout", a process that removes nuclei with abnormal mitoses, than wild-type embryos. Fallout nuclei tend to be observed in pairs or clusters and have a late telophase appearance. There is no evidence of metaphase defects in these embryos although a small number show chromatin bridges that persist to the prometaphase stage.
Somatic mitotic clones of Asx3 do not induce ovarian defects.
10% of heterozygotes show transformation of abdominal segment A4 to A5 (extra black pigmentation is seen on the fourth abdominal tergite in males). 0% of heterozygotes show transformation of abdominal segment A6 to A7 (males have a reduced sixth abdominal tergite). There is an average of 0.1 extra sex combs (average total number of extra sex comb teeth on the four posterior legs of a male).
Embryonic lethal or semi-lethal when heterozygous with ph-d2. Unhatched larvae carry segmentation defects or polarity defects.