FlyBase Allele Report: Dmel\lolal[k02512]

General Information

Symbol

Dmel\lolal^k02512

Species

D. melanogaster

Name

FlyBase ID

FBal0064584

Feature type

allele

Associated gene

Dmel\lolal

Associated Insertion(s)

P{lacW}lolal^k02512

Carried in Construct

Also Known As

l(2)k02512

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Faucheux et al., 2003)

Mutagen

P-element activity

(Roch et al., 1998, BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}lolal^k02512

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:18,156,939..18,156,939 [+]

(Quijano et al., 2016, Mishra et al., 2003, Spradling et al., 1999, Roch et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo (with Df(2R)Pcl11B)

(Quijano et al., 2016)

embryo | maternal effect (with lolal¹¹²²)

(Quijano et al., 2016)

presumptive embryonic salivary gland

(Kerman et al., 2008)

sex comb | ectopic

(Faucheux et al., 2003)

tracheal dorsal trunk primordium

(Kerman et al., 2008)

Detailed Description

Statement

Reference

lolal^k02512/lolal¹¹²², lolal^k02512/lolal¹⁷²² and lolal^k02512/Df(2R)Pcl11B transheterozygotes display lethality or near lethality, and lolal^k02512/lolal^G9603 transheterozygotes exhibit semi-lethality, as compared to controls, irrespective of the gender of the parental genotype; lolal^k02512/lolal¹¹²² and lolal^k02512/lolal¹⁷²² transheterozygotes mainly die during the pupal stage.

The progeny from lolal^k02512/+ fathers and either lolal¹¹²²/+ or Df(2R)Pcl11B/+ mothers exhibit embryo ventralization in a small proportion of cases, as compared to controls.

(Quijano et al., 2016)

The developing salivary glands in lolal^k02512 mutant animals fail to turn and migrate properly. In addition, the dorsal trunks of the trachea exhibit only partial extension at stage 14, but relatively normal extension of dorsal and visceral branches occurs.

(Kerman et al., 2008)

lolal^k02512 mutants exhibit cuticular defects. The position of the first abdominal denticle belt is moved posteriorly, indicating head involution defects associated with altered mouth hooks. lolal^k02512 mutants exhibit dorsal closure defects, to varying degrees of severity, from a hole in the dorsal cuticle to the absence of closure. lolal^k02512 embryos lacking both maternal and zygotic contribution of lolal display cuticular defects compared to the wild-type. Embryos show incomplete head involution with many exhibiting defects in dorsal closure. In contrast, embryos derived from lolal^k02512/lolal^k02512;Scer\GAL4^da.G32, lolal^{Scer\UAS.T:Avic\GFP-EGFP}/+ rescued females crossed to wild-type males hatch and do not display cuticular defects. Heteroallelic lolal^311a/lolal^k02512 flies have wild-type wings. lolal^Δ11π mutants, in combination with lolal^k02512 exhibit ectopic sex comb teeth on meso- or metathoracic legs in about one-fifth of the male escapers.

(Faucheux et al., 2003)

Lethality occurs during third instar larval or pupal stages. Mutants do not show any visible disc abnormalities.

(Roch et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

lolal^k02512/lolal[+] is an enhancer of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

lolal^k02512 is an enhancer of visible | recessive phenotype of ph-p⁴¹⁰

(Faucheux et al., 2003)

lolal^k02512 is an enhancer of visible | recessive phenotype of Pc¹⁶

(Faucheux et al., 2003)

Other

Statement

Reference

dpp^e87, lolal^k02512/lolal[+] has partially lethal - majority live | maternal effect phenotype

(Quijano et al., 2016)

dpp^hr27, lolal^k02512/lolal[+] has lethal | maternal effect phenotype

(Quijano et al., 2016)

dpp^hr4, lolal^k02512/lolal[+] has partially lethal - majority die | maternal effect phenotype

(Quijano et al., 2016)

dpp^hr56, lolal^k02512/lolal[+] has partially lethal - majority die | maternal effect phenotype

(Quijano et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

lolal^k02512 has presumptive embryonic salivary gland phenotype, enhanceable by rib[+]/rib¹

(Kerman et al., 2008)

NOT Enhanced by

Statement

Reference

lolal^k02512 has tracheal dorsal trunk primordium phenotype, non-enhanceable by rib[+]/rib¹

(Kerman et al., 2008)

lolal^k02512 has phenotype, non-enhanceable by Psc[+]/Psc¹

(Mishra et al., 2003)

lolal^k02512 has phenotype, non-enhanceable by Scm^R5-13/Scm[+]

(Mishra et al., 2003)

lolal^k02512 has phenotype, non-enhanceable by Trl[+]/Trl^13C

(Mishra et al., 2003)

lolal^k02512 has phenotype, non-enhanceable by ash1^B1/ash1[+]

(Mishra et al., 2003)

lolal^k02512 has phenotype, non-enhanceable by osa^unspecified/osa[+]

(Mishra et al., 2003)

lolal^k02512 has phenotype, non-enhanceable by trx[+]/trx^unspecified

(Mishra et al., 2003)

NOT suppressed by

Statement

Reference

lolal^k02512 has phenotype, non-suppressible by Psc[+]/Psc¹

(Mishra et al., 2003)

lolal^k02512 has phenotype, non-suppressible by Scm^R5-13/Scm[+]

(Mishra et al., 2003)

lolal^k02512 has phenotype, non-suppressible by ash1^B1/ash1[+]

(Mishra et al., 2003)

lolal^k02512 has phenotype, non-suppressible by osa^unspecified/osa[+]

(Mishra et al., 2003)

lolal^k02512 has phenotype, non-suppressible by trx[+]/trx^unspecified

(Mishra et al., 2003)

lolal^k02512 has phenotype, non-suppressible by Trl[+]/Trl^13C

(Mishra et al., 2003)

Enhancer of

Statement

Reference

lolal^k02512 is an enhancer of mesothoracic leg phenotype of ph-p⁴¹⁰

(Faucheux et al., 2003)

lolal^k02512 is an enhancer of sex comb tooth phenotype of Pc¹⁶

(Faucheux et al., 2003)

lolal^k02512 is an enhancer of metathoracic leg phenotype of ph-p⁴¹⁰

(Faucheux et al., 2003)

lolal^k02512 is an enhancer of prothoracic leg | ectopic phenotype of ph-p⁴¹⁰

(Faucheux et al., 2003)

lolal^k02512 is an enhancer of sex comb tooth phenotype of ph-p⁴¹⁰

(Faucheux et al., 2003)

lolal^k02512 is an enhancer of mesothoracic leg phenotype of Pc¹⁶

(Faucheux et al., 2003)

lolal^k02512 is an enhancer of metathoracic leg phenotype of Pc¹⁶

(Faucheux et al., 2003)

lolal^k02512 is an enhancer of prothoracic leg | ectopic phenotype of Pc¹⁶

(Faucheux et al., 2003)

NOT Enhancer of

Statement

Reference

lolal^k02512/lolal[+] is a non-enhancer of phenotype of Psc¹

(Mishra et al., 2003)

lolal^k02512/lolal[+] is a non-enhancer of phenotype of osa^unspecified

(Mishra et al., 2003)

lolal^k02512/lolal[+] is a non-enhancer of phenotype of Scm^R5-13

(Mishra et al., 2003)

lolal^k02512/lolal[+] is a non-enhancer of phenotype of trx^unspecified

(Mishra et al., 2003)

lolal^k02512/lolal[+] is a non-enhancer of phenotype of ash1^B1

(Mishra et al., 2003)

NOT Suppressor of

Statement

Reference

lolal^k02512/lolal[+] is a non-suppressor of phenotype of Psc¹

(Mishra et al., 2003)

lolal^k02512/lolal[+] is a non-suppressor of phenotype of Scm^R5-13

(Mishra et al., 2003)

lolal^k02512/lolal[+] is a non-suppressor of phenotype of trx^unspecified

(Mishra et al., 2003)

lolal^k02512/lolal[+] is a non-suppressor of phenotype of ash1^B1

(Mishra et al., 2003)

lolal^k02512/lolal[+] is a non-suppressor of phenotype of osa^unspecified

(Mishra et al., 2003)

Other

Statement

Reference

dpp^hr4, lolal^k02512/lolal[+] has embryo | maternal effect phenotype

(Quijano et al., 2016)

Pc¹, lolal^k02512 has wing phenotype

(Mishra et al., 2003)

Pc¹, lolal^k02512 has wing margin phenotype

(Mishra et al., 2003)

lolal^k02512, ph-p⁴¹⁰ has abdominal segment 6 phenotype

(Mishra et al., 2003)

lolal^k02512, ph-p⁴¹⁰ has abdominal segment 5 | ectopic phenotype

(Mishra et al., 2003)

lolal^k02512, ph-p⁴¹⁰ has abdominal segment 6 | ectopic phenotype

(Mishra et al., 2003)

lolal^k02512, ph-p⁴¹⁰ has abdominal segment 7 | ectopic phenotype

(Mishra et al., 2003)

lolal^k02512, mor¹ has eye phenotype

(Mishra et al., 2003)

lolal^k02512, trg^unspecified has abdominal segment 7 phenotype

(Mishra et al., 2003)

lolal^k02512, trg^unspecified has abdominal segment 6 | ectopic phenotype

(Mishra et al., 2003)

Asx^XF23, lolal^k02512 has abdominal segment 6 phenotype

(Mishra et al., 2003)

Asx^XF23, lolal^k02512 has abdominal segment 7 | ectopic phenotype

(Mishra et al., 2003)

lolal^k02512/lolal[+], ph-p⁴¹⁰ has sex comb | ectopic phenotype

(Mishra et al., 2003)

lolal^k02512/lolal[+], ph-p⁴¹⁰ has abdominal segment 4 phenotype

(Mishra et al., 2003)

lolal^k02512/lolal[+], ph-p⁴¹⁰ has abdominal segment 5 phenotype

(Mishra et al., 2003)

lolal^k02512/lolal[+], ph-p⁴¹⁰ has abdominal segment 6 phenotype

(Mishra et al., 2003)

lolal^k02512/lolal[+], ph-p⁴¹⁰ has abdominal segment 5 | ectopic phenotype

(Mishra et al., 2003)

lolal^k02512/lolal[+], ph-p⁴¹⁰ has abdominal segment 6 | ectopic phenotype

(Mishra et al., 2003)

lolal^k02512/lolal[+], ph-p⁴¹⁰ has abdominal segment 7 | ectopic phenotype

(Mishra et al., 2003)

lolal^k02512, ph-p⁴¹⁰ has sex comb | ectopic phenotype

(Mishra et al., 2003)

lolal^k02512, ph-p⁴¹⁰ has abdominal segment 4 phenotype

(Mishra et al., 2003)

lolal^k02512, ph-p⁴¹⁰ has abdominal segment 5 phenotype

(Mishra et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The progeny from lolal^k02512/+ mothers and dpp^hr4/+ fathers present both embryo ventralization in less than half of cases and near lethality, as compared to controls; the progeny from lolal^k02512/+ mothers and either dpp^hr27/+, dpp^hr56/+ or dpp^e87/+ fathers exhibit lethality, semi-lethality and increased mortality, respectively, as compared to controls.

(Quijano et al., 2016)

lolal^k02512 mutants show a higher proportion of salivary gland defects in combination with rib¹/+. In these animals the salivary glands fail to turn and migrate properly. However, tracheal dorsal trunk defects are not enhanced in these animals at stage 14.

(Kerman et al., 2008)

Adding at least one copy of the Trl^{Hsp83.GAGA-519} or Hsp83^GAGA581 transgenes allows the recovery of a much higher frequency of homozygous Trl^13C escapers that are also heterozygous for lolal^k02512 (up to approximately 80% when four copies of the transgene are added).

lolal^k02512 enhances the ph-p⁴¹⁰ esc-like phenotype, consisting of homeotic transformation of mesothoracic or metathoracic legs towards prothoracic legs due to the deregulation of Scr. Mutants heterozygous for lolal^k02512, when also heterozygous for ph-p⁴¹⁰, Pc¹⁶, Pc¹, Pc³ or Pc¹⁵, exhibit a contrabithorax phenotype, which is a wing-to-haltere transformation due to ectopic expression of Ubx in the posterior part of the wing disc, often resulting in curvature of the wing. This phenotype is further enhanced in the viable lolal^k02512/lolal^311a heteroallelic combinations, when also heterozygous for ph-p⁴¹⁰, Pc¹⁶, Pc¹, Pc³ or Pc¹⁵. lolal^k02512 ; Trl^13C heterozygous flies are viable and do not show any visible phenotype. Approximately 4% of lolal^k02512/+; Trl^13C/Trl^13C flies hatch. Adding at least one copy of the Hsp83^GAGA519 or Hsp83^GAGA581 transgenes allows the recovery of a much higher frequency of homozygous Trl^13C escapers that are also heterozygous for lolal^k02512 (up to approximately 80% when four copies of the transgene are added). Double heterozygous lolal^k02512 Ubx¹³⁰ flies do not show significant increase in the expressivity of the Ubx phenotype. lolal^k02512/+; Trl^13C/Ubx¹³⁰ flies display a higher frequency (18%) of strong haltere-to-wing transformations compared to Trl^13C/Ubx¹³⁰ siblings (6.3%). This increased transformation is often accompanied by a notal transformation.

(Faucheux et al., 2003)

The addition of lolal^k02512/+ to homozygous ph-p⁴¹⁰ animals shows a partial homeotic transformation of A4 to A5, A5 to A6, and A6 to A7. There is a strong reduction of the size of the 6th tergite. Sex combs are seen on the second and third legs. The addition of lolal^k02512/+ to heterozygous Asx^XF23 animals shows a partial homeotic transformation of A6 to A5 in the abdomen. The addition of lolal^k02512/+ to heterozygous Pc¹ animals produces crumbled wings with defective margin. The addition of lolal^k02512/+ to heterozygous mor¹ animals produces rough eyes. trg^unspecified/+, lolal^k02512/+ animals show development of a small additional tergite, indicating a transformation of A7 to A6. Trl^13C/+, lolal^k02512/+ animals show no enhancement or suppression of the lolal^k02512 phenotype. The addition of heterozygous osa^unspecified, trx^unspecified, ash1^B1, Psc¹ or Scm^R5-13 to lolal^k02512/+ animals shows enhancement or suppression.

(Mishra et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

lolal^k07907

(BDGP Project Members, 1994-1999)

lolal^k11212

(BDGP Project Members, 1994-1999)

Rescued by

lolal^k02512 is rescued by Scer\GAL4^da.G32/lolal^UAS.Tag:FLAG

(Faucheux et al., 2003)

lolal^k02512 is rescued by lolal^UAS.EGFP/Scer\GAL4^da.G32

(Faucheux et al., 2003)

lolal^k02512 is rescued by lolal^+tBB

(Faucheux et al., 2003)

Comments

Addition of lolal^+tBB, lolal^{Scer\UAS.T:Zzzz\FLAG} or lolal^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 where necessary, to ph-p⁴¹⁰, lolal^k02512 double mutants results in rescue of the enhancement of the ph-p⁴¹⁰ esc-like phenotype, with a reduction in the number of homeotic transformations of mesothoracic or metathoracic legs towards prothoracic legs and the mean number of sex comb teeth to a level similar to that in the double mutant ph-p⁴¹⁰, lolal^k02512.

(Faucheux et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10515

y¹ w^67c23; P{lacW}lolal^k02512/CyO

Kyoto

111150

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}lolal^k02512 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: Hsf⁰³⁰⁹¹. Complements: sbb⁰⁴⁴⁴⁰. Complements: l(2)04548⁰⁴⁵⁴⁸. Complements: l(2)k08931^k08931. Complements: pAbp^k10109. Complements: Pcl^s1859.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

25/12

(Roch et al., 1998)

ban^l(2)k02512

(Faucheux et al., 2003)

batman^l(2)k02512

(Bartoletti et al., 2012)

l(2)k02512

(Mutsuddi et al., 2004, Faucheux et al., 2003, Doane, 2000, Faucheux et al., 2000, Spradling et al., 1999)

l(2)k02512^k02512

(BDGP Project Members, 1994-1999)

lolal^K02512

(Quijano et al., 2016, Kerman et al., 2008)

lolal^KO2512

(Quijano et al., 2016)

lolal^[k02512]

(Bejarano and Busturia, 2004)

lolal^k02512

lolal^l(2)k02515

(Kerman et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (15)

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