FlyBase Allele Report: Dmel\crol[k05205]

General Information

Symbol

Dmel\crol^k05205

Species

D. melanogaster

Name

FlyBase ID

FBal0064729

Feature type

allele

Associated gene

Dmel\crol

Associated Insertion(s)

P{lacW}crol^k05205

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}crol^k05205

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | third instar larval stage

(Mitchell et al., 2008)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

wing pouch | somatic clone

(Mitchell et al., 2008)

Detailed Description

Statement

Reference

Homozygous clones are rarely seen in the wing pouch of wing discs from 120 hour larvae (clones induced at 72 hours after egg deposition), in contrast to control clones. Homozygous mutant clones are recovered in the hinge region of these discs.

Small homozygous clones are recovered in the wing pouch in 96 hour larval wing discs (clones induced at 48 hours after egg deposition). Clonal cells with a pyknotic morphology are seen in the basal section of the wing pouch in these discs. The homozygous cells show reduced BrdU incorporation and a reduction in the number of mitotic cells compared to wild-type clones.

(Mitchell et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

crol^k05205 has decreased occurrence of cell division | somatic clone | third instar larval stage phenotype, suppressible by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Mitchell et al., 2008)

Enhancer of

Statement

Reference

crol[+]/crol^k05205 is an enhancer of visible phenotype of Scer\GAL4^en-e16E, hfp^UAS.cQa

(Mitchell et al., 2008)

Suppressor of

Statement

Reference

crol[+]/crol^k05205 is a suppressor of visible | dominant phenotype of gcm^Pyx

(Popkova et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

crol^k05205 has wing pouch | somatic clone phenotype, suppressible by arm⁴/arm[+]

(Mitchell et al., 2008)

crol^k05205 has wing pouch | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Mitchell et al., 2008)

Enhancer of

Statement

Reference

crol[+]/crol^k05205 is an enhancer of wing phenotype of Scer\GAL4^en-e16E, hfp^UAS.cQa

(Mitchell et al., 2008)

Suppressor of

Statement

Reference

crol[+]/crol^k05205 is a suppressor of chaeta | increased number phenotype of gcm^Pyx

(Popkova et al., 2012)

crol[+]/crol^k05205 is a suppressor of eye phenotype of Dref^UAS.cSa

(Ida et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

crol^k05205/+ enhances the reduced wing phenotype caused by expression of pUf68^Scer\UAS.cQa under the control of Scer\GAL4^en-e16E in the posterior compartment.

Analysis of 120 hour larval wing discs indicates that the growth of crol^k05205 clones is restored if they are induced in a arm⁴/+ background compared to when they are induced in an otherwise wild-type background.

(Mitchell et al., 2008)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^tub in homozygous crol^k05205 clones results in more mutant cells being seen in the wing pouch of wing discs from 120 hour larvae (clones induced at 72 hours after egg deposition) compared to homozygous crol^k05205 clones induced in an otherwise wild-type background.

(Mitchell et al., 2008)

Complementation and Rescue Data

Fails to complement

crol⁰⁴⁴¹⁸

(BDGP Project Members, 1994-1999)

crol⁰⁶⁴⁷⁰

(BDGP Project Members, 1994-1999)

crol^k08217

(BDGP Project Members, 1994-1999)

crol^k09625

(BDGP Project Members, 1994-1999)

Rescued by