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General Information
Symbol
Dmel\Xbp1k13803
Species
D. melanogaster
Name
FlyBase ID
FBal0064120
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion at position +156 relative to the transcription start site.

Insertion components
P{lacW}Xbp1k13803
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

autophagic vacuole & larval fat body | somatic clone | cell autonomous

lysosome & larval fat body | somatic clone | cell autonomous

Detailed Description
Statement
Reference

Axon regeneration is mildly reduced in Xbp1k13803/Xbp1k13803 larvae and significantly reduced in Xbp1k13803/Df(2R)BSC484 larvae.

Xbp1k13803 homozygous midgut clones present a significantly increased cell number at 3 and 7 days after clone induction, as compared to control clones.

Heterozygous Xbp1k13803 mutants do not exhibit an increase in apoptosis.

Xbp1k13803 clones in fat bodies from well-fed early third instar larvae accumulate lysosomes, as indicated by elevated LysoTracker staining, and show redistribution of the Atg8a product into autophagosomes.

At day 4 of development, Xbp1k13803 larvae are significantly retarded in their growth compared to their heterozygous siblings.

Xbp1k13803/Df(2R)F36 mutant larvae lack imaginal discs.

95% of homozygotes hatch (these animals appear indistinguishable from controls at the time of hatching). The homozygous larvae remain at the first instar stage, become sluggish and sick before dying after two to four says.

Lethality occurs at an early stage of development, mutants are underdeveloped even under prolonged incubation periods.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhancer of
Statement
Reference

Xbp1[+]/Xbp1k13803 is an enhancer of wing margin phenotype of apXa

Xbp1[+]/Xbp1k13803 is an enhancer of wing margin phenotype of Bx2

Xbp1[+]/Xbp1k13803 is an enhancer of retina phenotype of ninaEG69D

Suppressor of
Statement
Reference

Xbp1[+]/Xbp1k13803 is a suppressor of eye phenotype of DrefUAS.cSa

Additional Comments
Genetic Interactions
Statement
Reference

Enhanced axon regeneration seen in RtcaNP5057/RtcaNP5057 larvae is dampened in RtcaNP5057;Xbp1k13803 double mutants.

A Xbp1k13803/+ background significantly accelerates the retinal degeneration of ninaEG69D/+ flies: the pseudopupil of approximately 30% of Xbp1k13803/+ ninaEG69D/+ flies starts to disappear by day 8 (i.e. 8 days earlier than in ninaEG69D/+ flies) and no pseudopupils are observed after day 16.

Xenogenetic Interactions
Statement
Reference

Xbp1k13803 induces early death in flies expressing Hsap\TOR1AΔE.Scer\UAS under the control of Scer\GAL4elav-C155 compared with controls.

A heterozygous Xbp1k13803 background significantly increases the level of apoptosis mediated by expression of Hsap\MAPTR406W.Scer\UAS under the control of Scer\GAL4elav-C155.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Lethality may be due to a specific larval function of the affected locus or is the consequence of the gene being required during embryonic development.

Precise excision of the insertion in Xbp1k13803 revert the mutant lethal phenotype.

Xbp1 transcript levels are severely reduced in 4-day-old Xbp1k13803 larvae.

Complements: l(2)0305003050. Complements: l(2)0720607206. Complements: shg10469. Complements: shgk03401. Complements: shgk10220.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
References (21)