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General Information
Symbol
Dmel\ninaEG69D
Species
D. melanogaster
Name
FlyBase ID
FBal0046173
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
rh1G69D
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G19887762A

Amino acid change:

G69D | ninaE-PA

Reported amino acid change:
Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: G69D. Nucleotide substitution: G377A.

Amino acid replacement: G69D.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
is exacerbated by hiroF1
is NOT ameliorated by hiroUAS.Tag:V5
is exacerbated by Xbp1k13803
is ameliorated by HerpGD981
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

ninaEG69D heterozygotes exhibit severe loss of rhabdomeres and photoreceptor cells, and small ERG response amplitudes and a complete loss of transients, ~25 days after eclosion.

ninaEG69D heterozygotes show prominent retinal degeneration, as compared to wild-type controls.

Mutant flies raised under a 12 hour day/light cycle do not show retinal degeneration.

Heterozygous flies show a progressive retinal degeneration.

Heterozygotes show progressive retinal degeneration (assayed by loss of the pseudopupil); the pseudopupils disappear beginning at 12 days after eclosion in a progressive manner, with only 10% of flies showing intact pseudopupils at 28 days after eclosion. In 21 day old flies, most ommatidia are in disarray, with large vacuoles and with an overall reduction of rhabdomere numbers.

Sixty-day old ninaEG69D homozygous mutant retina desiplay defects in the ommatidia trapezoidal arrangement and occasional photoreceptor cell loss.

The pseudopupil of ninaEG69D/+ flies starts to disappear, in a progressive manner, after 16 days. After 24 days, no ninaEG69D/+ flies with a pseudopupil are observed.

Severe retinal degradation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

ninaEG69D has retina phenotype, enhanceable by Xbp1[+]/Xbp1k13803

NOT Enhanced by
Statement
Reference

ninaEG69D has retina phenotype, non-enhanceable by Xbp1[+]/Xbp1excP

Suppressed by
Statement
Reference

ninaEG69D has retina phenotype, suppressible | partially by sip3GMR.cXa

ninaEG69D has retina phenotype, suppressible by sordd1GMR.cXa

ninaEG69D has retina | conditional phenotype, suppressible | partially by Mekk1Ur36/Mekk1Ur36

ninaEG69D has retina | conditional phenotype, suppressible | partially by Cdk5GD13840/Scer\GAL4GMR.PF

NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference

ninaEG69D/ninaE[+] is a suppressor of eye photoreceptor cell | somatic clone | cell autonomous | adult stage | conditional phenotype of Fatp1k10307

ninaEG69D is a suppressor of phenotype of norpA36

Additional Comments
Genetic Interactions
Statement
Reference

The retinal degeneration observed in ninaEG69D heterozygotes is exacerbated by hiroF1 homozygosity, but is not rescued by the expression of hiroScer\UAS.T:SV5\V5 under the control of Scer\GAL4ninaE.PT.

The presence of ninaEG69D/+ rescues the photoreceptor degeneration phenotype seen in Fatpk10307 clones in the retina.

The time course of retinal degeneration seen in ninaEG69D/+ flies is delayed in a Mekk1Ur36/Mekk1Ur36 background.

The time course of retinal degeneration seen in ninaEG69D/+ flies is delayed in by expression of Cdk5GD13840 under the control of Scer\GAL4GMR.PF.

Expression of sip3Scer\UAS.T:Hsap\MYC or Edem2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ninaE.PT dramatically decreases the rate of pseudopupil loss seen in ninaEG69D/+ flies. The ommatidial defects seen in 21 day old ninaEG69D/+ flies are also suppressed in the double mutants.

Expression of Edem1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ninaE.PT slightly delays the rate of pseudopupil loss seen in ninaEG69D/+ flies. The ommatidial defects seen in 21 day old ninaEG69D/+ flies are mildly suppressed in the double mutants.

Reduced ninaE dosage in ninaEG69D heterozygotes protects photoreceptor cells from Scer\GAL4ninaE.PT-p53Scer\UAS.cJa-induced apoptosis.

A Xbp1k13803/+ background significantly accelerates the retinal degeneration of ninaEG69D/+ flies: the pseudopupil of approximately 30% of Xbp1k13803/+ ninaEG69D/+ flies starts to disappear by day 8 (i.e. 8 days earlier than in ninaEG69D/+ flies) and no pseudopupils are observed after day 16.

A Xbp1excP/+ background has no effect on the degeneration of ninaEG69D/+ eyes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (14)