FlyBase Allele Report: Dmel\crc[MI02300-GFSTF.1]

The RMCE cassette of the Mi{MIC}crc^MI02300 insertion has been replaced with a EGFP-FlAsH-StrepII-TEV-3xFlag multi-tag cassette. The insertion is in a coding intron of crc and is predicted to protein trap the gene.

(Gene Disruption Project members, 2015-)

Allele components

Component

Use(s)

Inserted element

Mi{PT-GFSTF.1}

protein trap

Tagged with

EGFP

green fluorescent protein

Tag:CALI(TC)

chromophore-assisted light inactivation tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:21,315,563..21,315,563 [-]

(Vachias et al., 2025, Walsh et al., 2025, Chen and McDonald, 2024, Grmai et al., 2024, Vasudevan et al., 2022, Gene Disruption Project members, 2015-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size

(Vasudevan et al., 2022)

abnormal size (with crc¹)

(Vasudevan et al., 2022)

decreased cell number | oogenesis

(Vasudevan et al., 2022)

decreased cell number | oogenesis (with crc¹)

(Vasudevan et al., 2022)

decreased fecundity | female

(Vasudevan et al., 2022)

decreased fecundity | female (with crc¹)

(Vasudevan et al., 2022)

increased cell death | oogenesis

(Grmai et al., 2024, Vasudevan et al., 2022)

increased cell death | oogenesis (with crc¹)

(Vasudevan et al., 2022)

increased mortality | adult stage | nutrition conditional (with crc¹)

(Vasudevan et al., 2022)

increased size | adult stage

(Grmai et al., 2024)

majority die during larval stage

(Vasudevan et al., 2022)

majority die during larval stage (with crc¹)

(Vasudevan et al., 2022)

male fertile

(Vasudevan et al., 2022)

male fertile (with crc¹)

(Vasudevan et al., 2022)

partially lethal - majority die

(Vasudevan et al., 2022)

partially lethal - majority die (with crc¹)

(Vasudevan et al., 2022)

some die during P-stage

(Vasudevan et al., 2022)

some die during P-stage (with crc¹)

(Vasudevan et al., 2022)

visible

(Vasudevan et al., 2022)

visible (with crc¹)

(Vasudevan et al., 2022)

Phenotype Manifest In

adult fat body

(Grmai et al., 2024)

dorsal appendage | maternal effect

(Vasudevan et al., 2022)

dorsal appendage | maternal effect (with crc¹)

(Vasudevan et al., 2022)

egg chamber

(Vasudevan et al., 2022)

egg chamber (with crc¹)

(Vasudevan et al., 2022)

egg chamber | decreased number

(Grmai et al., 2024)

lipid droplet | adult stage

(Grmai et al., 2024)

ovary

(Vasudevan et al., 2022)

ovary (with crc¹)

(Vasudevan et al., 2022)

retina (with crc¹)

(Vasudevan et al., 2022)

stalk follicle cell | decreased number

(Vasudevan et al., 2022)

stalk follicle cell | decreased number (with crc¹)

(Vasudevan et al., 2022)

wing blade

(Vasudevan et al., 2022)

wing blade (with crc¹)

(Vasudevan et al., 2022)

wing vein | ectopic

(Vasudevan et al., 2022)

wing vein | ectopic (with crc¹)

(Vasudevan et al., 2022)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

crc¹/crc^{MI02300-GFSTF.1}, ninaE^G69D/ninaE[+] has abnormal neuroanatomy | adult stage | progressive phenotype

(Vasudevan et al., 2022)

crc^{MI02300-GFSTF.1}, ninaE^G69D/ninaE[+] has abnormal neuroanatomy | adult stage phenotype

(Vasudevan et al., 2022)

Phenotype Manifest In

Other

Statement

Reference

crc¹/crc^{MI02300-GFSTF.1}, ninaE^G69D/ninaE[+] has retina phenotype

(Vasudevan et al., 2022)

crc¹/crc^{MI02300-GFSTF.1}, ninaE^G69D/ninaE[+] has ommatidium | adult stage phenotype

(Vasudevan et al., 2022)

crc¹/crc^{MI02300-GFSTF.1}, ninaE^G69D/ninaE[+] has eye photoreceptor cell | adult stage phenotype

(Vasudevan et al., 2022)

crc^{MI02300-GFSTF.1}, ninaE^G69D/ninaE[+] has retina phenotype

(Vasudevan et al., 2022)

crc^{MI02300-GFSTF.1}, ninaE^G69D/ninaE[+] has ommatidium | adult stage phenotype

(Vasudevan et al., 2022)

crc^{MI02300-GFSTF.1}, ninaE^G69D/ninaE[+] has eye photoreceptor cell | adult stage phenotype

(Vasudevan et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

crc^{MI02300-GFSTF.1} is rescued by crc^UAS.cKa/Scer\GAL4^Lsp2.3.1

(Grmai et al., 2024)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59608

y¹ w^67c23; Mi{PT-GFSTF.1}crc^{MI02300-GFSTF.1}

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

crc^GFSTF

(Walsh et al., 2025, Grmai et al., 2024, Vasudevan et al., 2022)

crc^{MI02300-GFSTF.1}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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