FlyBase Allele Report: Dmel\Cdk5[GD13840]

General Information

Symbol

Dmel\Cdk5^GD13840

Species

D. melanogaster

Name

FlyBase ID

FBal0206542

Feature type

allele

Associated gene

Dmel\Cdk5

Associated Insertion(s)

Carried in Construct

P{GD13840}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD13840}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD13840

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}

(Liman et al., 2014)

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Liman et al., 2014)

ameliorates retinal disease

modeled by ninaE^G69D.UAS

(Kang et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

short lived, with Scer\GAL4^elav-C155

(Liman et al., 2014)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Pan-neural expression of Cdk5^GD13840 under the control of Scer\GAL4^elav-C155 has a minor effect on longevity (a 16% reduction in medial survival).

(Liman et al., 2014)

Adults expressing Cdk5^GD13840 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Adults expressing Cdk5^GD13840 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13840

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Cdk5^GD13840, Scer\GAL4^elav-C155 is an enhancer of short lived phenotype of Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}, Scer\GAL4^elav-C155

(Liman et al., 2014)

Suppressor of

Statement

Reference

Cdk5^GD13840/Scer\GAL4^unspecified is a suppressor of visible | adult stage phenotype of ninaE^P37H.GMR

(Park et al., 2023)

Cdk5^GD13840/Scer\GAL4^unspecified is a suppressor of decreased size | adult stage phenotype of ninaE^P37H.GMR

(Park et al., 2023)

Cdk5^GD13840, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, ninaE^G69D.UAS

(Kang et al., 2012)

Cdk5^GD13840, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, ninaE^G69D.UAS

(Kang et al., 2012)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Cdk5^GD13840, Scer\GAL4^GMR.long is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Liman et al., 2014)

Suppressor of

Statement

Reference

Cdk5^GD13840/Scer\GAL4^unspecified is a suppressor of eye phenotype of ninaE^P37H.GMR

(Park et al., 2023)

Cdk5^GD13840/Scer\GAL4^GMR.PF is a suppressor | partially of retina | conditional phenotype of ninaE^G69D

(Kang et al., 2012)

Cdk5^GD13840, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, ninaE^G69D.UAS

(Kang et al., 2012)

Cdk5^GD13840, Scer\GAL4^GMR.PF is a suppressor of eye disc phenotype of Scer\GAL4^GMR.PF, ninaE^G69D.UAS

(Kang et al., 2012)

NOT Suppressor of

Statement

Reference

Cdk5^GD13840, Scer\GAL4^GMR.long is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Liman et al., 2014)

Other

Statement

Reference

Cdk5^GD13840, Hsap\ATXN3^{fl.Q84.UAS.Tag:MYC}, Scer\GAL4^GMR.long has eye phenotype

(Liman et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The eye defects caused by expression of ninaE^{G69D.Scer\UAS} under the control of Scer\GAL4^GMR.PF are strongly suppressed by co-expression of Cdk5^GD13840. The massive apoptosis seen in the larval eye discs of animals expressing ninaE^{G69D.Scer\UAS} under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of Cdk5^GD13840.

The time course of retinal degeneration seen in ninaE^G69D/+ flies is delayed in by expression of Cdk5^GD13840 under the control of Scer\GAL4^GMR.PF.

(Kang et al., 2012)

Xenogenetic Interactions

Statement

Reference

Reduction of Cdk5 transcript abundance, through the expression of Cdk5^GD13840 does not affect the eye degeneration seen in eyes expressing Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} (all transgenes under the control of Scer\GAL4^GMR.long).

Reduction of Cdk5 transcript abundance, through the expression of Cdk5^GD13840 in eyes expressing Hsap\ATXN3^{fl.Q84.Scer\UAS.T:Hsap\MYC} (all transgenes under the control of Scer\GAL4^GMR.long) causes a disturbed eye surface and a loss of pigmentation.

RNAi-mediated Cdk5 reduction in Hsap\ATXN3^{fl.Q84.Scer\UAS.T:Hsap\MYC}-expressing flies (under the control of Scer\GAL4^elav-C155) results in an almost 50% reduction of median survival compared to controls.

(Liman et al., 2014)

Co-expression of Cdk5^GD13840 does not affect the rough eye phenotype caused by expression of p53^GUS.PB under the control of Scer\GAL4^GMR.PF.

(Kang et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v35855

w¹¹¹⁸; P{GD13840}v35855

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13840

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Cdk5^GD13840

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close