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General Information
Symbol
Dmel\ninaEG69D.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0230353
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
uas-Rh1G69D, UAS-Rh-1G69D
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: G69D.

UASt regulatory sequences drive expression of the ninaE product with a transmembrane glycine substituted to an aspartic acid residue (G69D).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
model of  retinal disease
is ameliorated by Diap1EY00710
is exacerbated by Mekk1EY02276
is ameliorated by Mekk1Ur36
is ameliorated by sip3EY11980
is ameliorated by Cdk5GD13840
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ninaEG69D.UAS under the control of Scer\GAL4GMR.PF leads to retinal degeneration (monitored using eye size) and ER stress in the eye. It also leads to increased apoptosis in eye discs.

Expression of ninaEG69D.UAS under the control of Scer\GAL4Bx-MS1096-KE leads to ER stress and apoptosis, resulting in a small, degenerate wing that fails to unfold upon eclosion.

Expression of ninaEG69D.Scer\UAS under the combined control of Scer\GAL4ninaE.PU and Scer\GAL4GMR.PU leads to age-progressive retinal degeneration in adult flies, the phenotype is milder in flies kept on a low-protein diet.

Expression of ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF results in significantly reduced adult eye size and decreased eye pigmentation.

Projection neurons expressing ninaEG69D.Scer\UAS under the control of Scer\GAL4GH146 do not show defects in targeting.

Flies expressing ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF have small eyes, and the surviving eye tissue has a glassy surface which is devoid of ommatidial structures. Larval eye discs show a massive amount of apoptosis in these animals.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The age-progressive retinal degeneration characteristic for adult flies expressing ninaEG69D.Scer\UAS under the combined control of Scer\GAL4ninaE.PU and Scer\GAL4GMR.PU is further worsened by co-expression of Gcn2KK103566 and unlike in the flies expressing only ninaEG69D.Scer\UAS, the phenotype cannot be ameliorated by keeping the flies on a low-protein diet.

The small eye phenotype characteristic for adult flies expressing ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced further by co-expression of any of the following: CG2004GD9700, fredKK103938, lolaKK110256, CG15666KK102315, ProsapKK101537, CG16885KK112024; not changed by co-expression of any one of these: hppyKK101460, Pde1cKK107045, CG43795KK115544, CG1785KK102592, Cdk5KK109344 and partially suppressed by co-expression of either Adgf-DKK104007 or CG31468KK110989. Co-expression of either Cdk5KK109344 or hppyKK101460 however have a reproducible qualitative effect on the ninaEG69D.Scer\UAS RNAi-induced eye phenotype: co-expression of Cdk5KK109344 improves the pigmentation defect, while hppyKK101460 expression results in the appearance of black necrotic spots.

Expression of ninaEG69D.Scer\UAS under the control of Scer\GAL4GH146 enhances the targeting defects seen in homozygous meigo1 projection neuron clones.

The eye defects caused by expression of ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF are strongly suppressed by co-expression of Cdk5GD13840. The massive apoptosis seen in the larval eye discs of animals expressing ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by co-expression of Cdk5GD13840.

The massive apoptosis seen in the larval eye discs of animals expressing ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by Cdk5α20C/Df(2L)p35-C2 but is not suppressed by crcR6/crc1.

The apoptosis seen in the larval eye discs of animals expressing ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced by Ire1f02170.

Mekk1Ur36/Mekk1Ur36 partially suppresses the eye defects caused by expression of ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF. The massive apoptosis seen in the larval eye discs of animals expressing ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by Mekk1Ur36/Mekk1Ur36.

The number of apoptotic cells is reduced in homozygous Df(2L)flp170B clones in eye discs expressing ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ninaEG69D.Scer\UAS
ninaEG69D.UAS
Name Synonyms
Secondary FlyBase IDs
    References (10)