FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Cdk5α20C
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General Information
Symbol
Dmel\Cdk5α20C
Species
D. melanogaster
Name
FlyBase ID
FBal0230266
Feature type
allele
Associated gene
Dmel\Cdk5α
Associated Insertion(s)
Carried in Construct
Also Known As
p3520C
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Connell-Crowley et al., 2007)
Mutagen
P-element activity
(Connell-Crowley et al., 2007)
Progenitor genotype
P{lacW}P100
(Connell-Crowley et al., 2007)
Cytology
Description

Cdk5α20C originates from the imprecise excision of the P{lacW}P100 insertion and it deletes about 90% of the Cdk5α coding region, including all sequences required for binding to and activating the Cdk5 product.

(Connell-Crowley et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  neurodegenerative disease
      CEA
      (Wodrich et al., 2024)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Cdk5α20C/Df(2L)p35-C2 adults at young age exhibit similar number of Mushroom Body (MB) neurons and negative gravitaxis abilities while at old age they exhibit a significant decrease in the number of MB neurons and in their negative gravitaxis abilities compared to controls. Cdk5α20C/Df(2L)p35-C2 adults retaining the most motor function at middle age (as assayed by wall climbing) tend to have more remaining MB neurons than the feeblest flies.

      Cdk5α20C/Df(2L)p35-C2 adults are more susceptible to oxidative stress (induced by feeding with paraquat or hydrogen peroxide) compared to controls.

      (Spurrier et al., 2018)

      Cdk5α20C/Df(2L)p35-C2 transheterozygous adults display significantly reduced adult lifespan and aged adults display more severe neurodegenerative phenotype (compromised tissue integrity particularly in the mushroom body) relative to control flies of comparable physiological age (= at equivalent stage of population mortality) but this effect is not due to either increased apoptosis or necrosis. The aged mutant adults however accumulate more autophagosomal organelles relative to controls. Cdk5α20C/Df(2L)p35-C2 adult also display significantly reduced survival rate upon starvation.

      Single-cell Cdk5α20C MARCM clones in the adult mushroom body initially display very low frequency of morphological abnormalities compared to controls but in aged flies show gross swelling of the proximal region of the axon in all three classes of adult Kenyon cells.

      (Trunova and Giniger, 2012)

      Approximately 50% of Cdk5α20C mutant embryos lacking both maternal and zygotic Cdk5α exhibit motor nerve axonal aberrations and guidance errors at modest expressivity. These defects include overextended intersegmental nerves, missing dorsal "a" branch of segmental nerves without the lateral branch being affected, missing "b" branch of intersegmental nerves and stalled transverse nerves. Adult Cdk5α20C mutants exhibit progressive loss of motor coordination.

      The lifespan of Cdk5α20C or Cdk5α20C/Df(2L)J77 flies is reduced by about one third relative to controls.

      (Connell-Crowley et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      NOT suppressed by
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      Cdk5α20C/Df(2L)p35-C2 is a suppressor of increased cell death phenotype of Scer\GAL4GMR.PF, ninaEG69D.UAS

      (Kang et al., 2012)
      NOT Suppressor of
      Phenotype Manifest In
      NOT Enhanced by
      NOT suppressed by
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      Cdk5α20C/Df(2L)p35-C2 is a suppressor of eye disc phenotype of Scer\GAL4GMR.PF, ninaEG69D.UAS

      (Kang et al., 2012)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The massive apoptosis seen in the larval eye discs of animals expressing ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by Cdk5α20C/Df(2L)p35-C2.

      (Kang et al., 2012)

      The motonerve defect phenotype of Cdk5α20C mutant embryos is not significantly different from the phenotype observed in Cdk5α20C mutant embryos also expressing Cdk5K33A.Scer\UAS.T:Zzzz\FLAG, a dominant negative form of Cdk5.

      (Connell-Crowley et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Cdk5α20C/Df(2L)p35-C2 is rescued by Cdk5α+t5.5

      (Spurrier et al., 2018)

      Cdk5α20C is rescued by Cdk5α+t5.5

      (Trunova and Giniger, 2012)

      Cdk5α20C is rescued by Cdk5α+t5.5

      (Connell-Crowley et al., 2007)
      Comments

      The age-progressive neurodegeneration (particularly in the mushroom body of the central brain) is rescued by combination with Cdk5α+t5.5.

      (Trunova and Giniger, 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      References (10)