FlyBase Allele Report: Dmel\Cdk5[K33A.UAS.Tag:FLAG]

General Information

Symbol

Dmel\Cdk5^{K33A.UAS.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0117901

Feature type

allele

Associated gene

Dmel\Cdk5

Associated Insertion(s)

Carried in Construct

P{UAS-Cdk5^K33A-FLAG}

Key Links

Transgenic product class

missense_variant

(Connell-Crowley et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Connell-Crowley et al., 2000)

Mutagen

in vitro construct

(Connell-Crowley, 2000, Connell-Crowley et al., 2000)

Progenitor genotype

Cdk5^UAS.Tag:FLAG

(Connell-Crowley, 2000, Connell-Crowley et al., 2000)

Carried in construct

P{UAS-Cdk5^K33A-FLAG}

Cytology

Description

Amino acid replacement: K33A.

(Connell-Crowley et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Cdk5

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^unspecified

(Connell-Crowley et al., 2007)

short lived, with Scer\GAL4^elav-C155

(Connell-Crowley et al., 2007)

Phenotype Manifest In

motor neuron | embryonic stage, with Scer\GAL4^unspecified

(Connell-Crowley et al., 2007)

presumptive embryonic/larval nervous system, with Scer\GAL4^unspecified

(Connell-Crowley et al., 2000)

Detailed Description

Statement

Reference

48% of the embryos expressing the dominant negative Cdk5^{K33A.Scer\UAS.T:Zzzz\FLAG} display motonerve defects.

A 29% reduction in median adult lifespan is observed when the dominant negative Cdk5^{K33A.Scer\UAS.T:Zzzz\FLAG} is expressed in postmitotic neurons under control of the Scer\GAL4^elav-C155 driver.

(Connell-Crowley et al., 2007)

Expression of Cdk5^{K33A.Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^unspecified has no effect on neuron cell fate. Two copies of P{UAS-Cdk5^K33A-FLAG} causes subtle nerve defects in the embryo. Four copies increases the proportion of embryos with defects.

(Connell-Crowley et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Cdk5^{K33A.UAS.Tag:FLAG}, Scer\GAL4^unspecified has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Cdk5α^20C

(Connell-Crowley et al., 2007)

NOT suppressed by

Statement

Reference

Cdk5^{K33A.UAS.Tag:FLAG}, Scer\GAL4^unspecified has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Cdk5α^20C

(Connell-Crowley et al., 2007)

NOT Enhancer of

Statement

Reference

Cdk5^{K33A.UAS.Tag:FLAG}/Scer\GAL4^unspecified is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of Cdk5α^20C

(Connell-Crowley et al., 2007)

NOT Suppressor of

Statement

Reference

Cdk5^{K33A.UAS.Tag:FLAG}/Scer\GAL4^unspecified is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Cdk5α^20C

(Connell-Crowley et al., 2007)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Cdk5^{K33A.UAS.Tag:FLAG}, Scer\GAL4^unspecified has motor neuron | embryonic stage phenotype, non-enhanceable by Cdk5α^20C

(Connell-Crowley et al., 2007)

NOT suppressed by

Statement

Reference

Cdk5^{K33A.UAS.Tag:FLAG}, Scer\GAL4^unspecified has motor neuron | embryonic stage phenotype, non-suppressible by Cdk5α^20C

(Connell-Crowley et al., 2007)

NOT Enhancer of

Statement

Reference

Cdk5^{K33A.UAS.Tag:FLAG}/Scer\GAL4^unspecified is a non-enhancer of motor neuron | embryonic stage phenotype of Cdk5α^20C

(Connell-Crowley et al., 2007)

Suppressor of

Statement

Reference

Cdk5^{K33A.UAS.Tag:FLAG}/Scer\GAL4^unspecified is a suppressor of presumptive embryonic/larval nervous system phenotype of Cdk5α^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Connell-Crowley et al., 2000)

Cdk5^{K33A.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Cdk5α^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Connell-Crowley et al., 2000)

NOT Suppressor of

Statement

Reference

Cdk5^{K33A.UAS.Tag:FLAG}/Scer\GAL4^unspecified is a non-suppressor of motor neuron | embryonic stage phenotype of Cdk5α^20C

(Connell-Crowley et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The motonerve defect phenotype of embryos expressing the dominant negative Cdk5^{K33A.Scer\UAS.T:Zzzz\FLAG} is not significantly different from the phenotype observed in Cdk5α^20C mutant embryos also expressing Cdk5^{K33A.Scer\UAS.T:Zzzz\FLAG}.

(Connell-Crowley et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Coexpression of Cdk5^{K33A.Scer\UAS.T:Zzzz\FLAG} and Cdk5^{Scer\UAS.T:Zzzz\FLAG} cause a smaller percentage of embryos to exhibit defects in the embryonic nervous system than Cdk5^{K33A.Scer\UAS.T:Zzzz\FLAG} alone.

(Connell-Crowley et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cdk5^{K33A.Scer\UAS.T:Zzzz\FLAG}

Cdk5^{K33A.UAS.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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