FlyBase Allele Report: Dmel\ap[Xa]

General Information

Symbol

Dmel\ap^Xa

Species

D. melanogaster

Name

Xasta

FlyBase ID

FBal0000663

Feature type

allele

Associated gene

Dmel\ap

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

Progenitor genotype

Caused by aberration

T(2;3)ap^Xa

Cytology

Description

A reciprocal translocation involving the right arms of the second and third chromosomes causes a breakpoint just upstream of the ap-RXa wing enhancer that juxtaposes the Dad and ap loci.

(Bieli et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | third instar larval stage (with ap^DG3)

(Bieli et al., 2015)

fertile

lethal | recessive

visible (with ap^DG3)

(Bieli et al., 2015)

visible | dominant

(Jack and DeLotto, 1992, Stevens, 1991)

visible | dominant | heat sensitive

(Bieli et al., 2015)

Phenotype Manifest In

mesonotum

(Stevens, 1991)

scutum

wing

(Jack and DeLotto, 1992, Stevens, 1991)

wing (with ap^DG3)

(Bieli et al., 2015)

wing blade | heat sensitive

(Bieli et al., 2015)

wing margin

(Bronstein et al., 2010)

wing margin bristle (with ap^DG3)

(Bieli et al., 2015)

wing pouch | third instar larval stage (with ap^DG3)

(Bieli et al., 2015)

Detailed Description

Statement

Reference

All ap^Xa/+ flies show a dominant wing phenotype: the distal part of the wing blade is lost and the characteristic mitten-shaped wing phenotype is formed.

The wing tissue of ap^Xa/ap^DG3 flies forms a short tube-like structure. Margin bristles are absent except for sometimes a few at the tip.

The dimension of the wing pouch in ap^Xa/ap^DG3 animals is reduced to about half the size of a wild-type pouch.

(Bieli et al., 2015)

Heterozygous flies exhibit severe wing notching.

(Bronstein et al., 2010)

Homozygotes exhibit loss of a large portion of the wing distal half removing the wing tip. The margin of the remaining wing is intact. The phenotype is unchanged in double homozygote combinations with ct^46l and ct^53d.

(Jack and DeLotto, 1992)

ap^Xa/ap⁺ flies produce a mitten-shaped wing. Duplications of the notum are often seen accompanied by loss of wing tissue, the duplications resemble notum duplications produced by wg mutations.

(Stevens, 1991)

Combination of NX¹ and ap^Xa produces small wings, like vg¹ and lower viability. Wings reduced in length to about 70% normal; irregular in outline with a V-shaped incision with apex at L2, uniformly present giving wing a mitten-like shape with the thumb between marginal vein and L2. Usually lethal in homozygous conditions, but occasionally ecloses very late as pale dwarf with wings and balancers like vg. Deep notch visible in tip of wing fold in prepupa (FBrf0004792; FBrf0005070). In homozygotes and in combination with ap⁴, ap⁶, or Df(2R)M41A4, wings are straplike and 30-70% normal length, and haltere length is 25-50% normal; longevity and fertility like ap⁴/ap⁴ except for an occasional long-lived ap^Xa/Df(2R)M41A4 female that may be fertile (FBrf0017003). In heterozygous combination with ap^ID, duplications of the notum occur frequently. Wing disc cell death found in both ap^Xa/+ (FBrf0020826) and ap^Xa/ap^ID (FBrf0031010).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

NX¹, ap^Xa has visible phenotype

Phenotype Manifest In

Enhanced by

Statement

Reference

ap^Xa has wing margin phenotype, enhanceable by Ssdp^L7/Ssdp[+]

(Bronstein et al., 2010)

ap^Xa has wing margin phenotype, enhanceable by CG11893^c03576/CG11893[+]

(Bronstein et al., 2010)

ap^Xa has wing margin phenotype, enhanceable by Xbp1[+]/Xbp1^k13803

(Bronstein et al., 2010)

NOT Enhanced by

Statement

Reference

ap^Xa has wing margin phenotype, non-enhanceable by CG1518[+]/Stt3A^KG03333

(Bronstein et al., 2010)

ap^Xa has wing margin phenotype, non-enhanceable by Cyp6d4[+]/Cyp6d4^EY04319

(Bronstein et al., 2010)

Suppressed by

Statement

Reference

ap^Xa has wing margin phenotype, suppressible by Kat60^EY05593/katanin-60[+]

(Bronstein et al., 2010)

ap^Xa has wing margin phenotype, suppressible by CtsF^EY02656/CG12163[+]

(Bronstein et al., 2010)

ap^Xa has wing margin phenotype, suppressible by CG12163[+]/CtsF^EY04405

(Bronstein et al., 2010)

ap^Xa has wing margin phenotype, suppressible by Mf^EY03476/Mf[+]

(Bronstein et al., 2010)

NOT suppressed by

Statement

Reference

ap^Xa has wing margin phenotype, non-suppressible by CG1518[+]/Stt3A^KG03333

(Bronstein et al., 2010)

ap^Xa has wing margin phenotype, non-suppressible by Cyp6d4[+]/Cyp6d4^EY04319

(Bronstein et al., 2010)

Other

Statement

Reference

NX¹, ap^Xa has wing phenotype

Additional Comments

Genetic Interactions

Statement

Reference

The wings of trans-heterozygous ap^Xa/+ ; Df(3R)Exel6176/+ animals display the same mitten-shaped wing phenotype as seen in ap^Xa heterozygous flies.

The wings of trans-heterozygous ap^Xa/+ ; Df(3)Dad^P1883Δ32/+ animals display the same mitten-shaped wing phenotype as seen in ap^Xa heterozygous flies.

(Bieli et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (1)

Holtzman, S. and Kaufman, T. (2013)

Mutant

Wild-type