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General Information
Symbol
Dmel\RafE1
Species
D. melanogaster
Name
FlyBase ID
FBal0051293
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
D-rafE1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C2342991T

Amino acid change:

P594S | Raf-PA; P594S | Raf-PE

Reported amino acid change:

P637S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: P637S.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Temperature shift experiments demonstrate the latest temperature-sensitive period for the requirement of phl during development is at the late pupal stage. Downshift experiment shows that embryos never hatch even when they were transferred to 17oC at 3 hours AEL at 28oC. The temperature-sensitive period for maternal phl function is 60 minutes AEL at 28oC and 260 minutes AEL at 17oC. Temperature shift up to 28oC within three days after puparium formation causes a failure to develop to mature adult within the pupal case, a raise in temperature after this period allows development to mature adult but individuals exhibit an eclosion defect. Mitotic clones demonstrate a proliferation defect, imaginal discs exhibit growth retardation. At 28oC the cell cycle is arrested at a phase or phases other than the M-phase.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference

phl[+]/RafE1 is a suppressor of visible phenotype of DrefUAS.cSa, Scer\GAL4en-e16E

Phenotype Manifest In
Suppressor of
Statement
Reference

phl[+]/RafE1 is a suppressor of wing vein L5 | distal phenotype of DrefUAS.cSa, Scer\GAL4en-e16E

Additional Comments
Genetic Interactions
Statement
Reference

phlE1/+ significantly suppresses the extra vein formation seen in flies expressing DrefScer\UAS.cSa under the control of Scer\GAL4en-e16E.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)