FlyBase Allele Report: Dmel\Rab11[N124I.UAS]

General Information

Symbol

Dmel\Rab11^N124I.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0190955

Feature type

allele

Associated gene

Dmel\Rab11

Associated Insertion(s)

Carried in Construct

P{UAS-Rab11.N124I}

Also Known As

UAS-Rab11^N124I, UAS-Rab11^DN, Rab11^DN, Rab11^N124I

Key Links

Transgenic product class

missense_variant

(Satoh et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Satoh et al., 2005)

Mutagen

in vitro construct

(Satoh et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Rab11.N124I}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form of Rab11 (carries the amino acid replacement N124I).

(Satoh et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rab11

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.cHa

(Deshpande et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval hemocyte | increased number | third instar larval stage

(Del Signore et al., 2017)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

(Korkut et al., 2013)

embryonic/larval tracheal dorsal trunk, with Scer\GAL4^69B

(Tiklová et al., 2010)

garland cell, with Scer\GAL4^hs.PB

(Satoh et al., 2005)

hypodermal muscle cell of A1-7 | third instar larval stage, with Scer\GAL4^elav-C155

(Korkut et al., 2013)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

larval tracheal system | embryonic stage, with Rab11^{S25N.UASp.YFP}, Scer\GAL4^btl.PS

(Tsarouhas et al., 2023)

larval tracheal system | embryonic stage, with Scer\GAL4^btl.PS

(Tsarouhas et al., 2023)

multivesicular body, with Scer\GAL4^hs.PB

(Satoh et al., 2005)

ommatidium, with Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

photoreceptor neuron, with Scer\GAL4^hs.PB

(Satoh et al., 2005)

rhabdomere, with Scer\GAL4^hs.PB

(Satoh et al., 2005)

tracheal lumen | embryonic stage, with Rab11^{S25N.UASp.YFP}, Scer\GAL4^btl.PS

(Tsarouhas et al., 2023)

tracheal lumen | embryonic stage, with Scer\GAL4^btl.PS

(Tsarouhas et al., 2023)

Detailed Description

Statement

Reference

Scer\GAL4^He.PZ-driven expression of Rab11^{N124I.Scer\UAS} induces increase in the number of circulating hemocytes in third instar larvae relative to controls.

(Del Signore et al., 2017)

Expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^VGlut.PD leads to excessive growth of neuromuscular junctions (increased NMJ area, length of the longest branch as well as the number of boutons) and slightly decreased crawling speed in third instar larvae.

(Deshpande et al., 2016)

Embryos expressing Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^69B demonstrate over-elongated dorsal trunks.

(Tiklová et al., 2010)

Only one or two bristles are seen in adult eyes of flies expressing Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF. There is a severe degeneration of ommatidial cell arrangement in animals expressing Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

Expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in enhanced cell death posterior to the morphogenetic furrow compared to controls.

(Tiwari and Roy, 2009)

Expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^hs.PB during pupal development reduces rhabdomere size and causes proliferation of abnormal cytoplasmic vesicles in the photoreceptor cells. In addition, these cells lack multivesicular bodies. Expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^hs.PB inhibits endocytosis in Garland cells (as shown by a failure to take up avidin). The labyrinthine channels, which normally invaginate deeply from the plasma membrane, are lost in these mutant Garland cells.

(Satoh et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by bsk²/bsk[+]

(Tiwari and Roy, 2009)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by msn¹⁷²/msn[+]

(Tiwari and Roy, 2009)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible by bsk^K53R.UAS, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible by puc^UAS.cMa, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Suppressor of

Statement

Reference

Rab11^N124I.UAS, Scer\GAL4^VGlut1.PD is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Rab11^N124I.UAS, Scer\GAL4^VGlut1.PD is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Rab11^N124I.UAS/Scer\GAL4^VGlut1.PD is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of Df(2R)BSC610/TBPH^Δ23

(Deshpande et al., 2016)

Other

Statement

Reference

Ocrl^Δ3, Rab11^N124I.UAS, Scer\GAL4^He.PZ has lethal - all die before end of larval stage phenotype

(Del Signore et al., 2017)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF, rpr^GMR.PH has visible phenotype

(Tiwari and Roy, 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Traf4^EP578, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by egr^UAS.cIa, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Tak1^UAS.cTa, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by puc^E69/puc[+]

(Tiwari and Roy, 2009)

Suppressed by

Statement

Reference

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by bsk²/bsk[+]

(Tiwari and Roy, 2009)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by msn¹⁷²/msn[+]

(Tiwari and Roy, 2009)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by bsk^K53R.UAS, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by puc^UAS.cMa, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Suppressor of

Statement

Reference

Rab11^N124I.UAS, Scer\GAL4^VGlut1.PD is a suppressor | partially of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Rab11^N124I.UAS, Scer\GAL4^VGlut1.PD is a suppressor | partially of NMJ bouton | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Other

Statement

Reference

Rab11^N124I.UAS, Rab27^{T25N.UASp.YFP}, Scer\GAL4^btl.PS has tracheal lumen | embryonic stage phenotype

(Tsarouhas et al., 2023)

Rab11^N124I.UAS, Rab27^{T25N.UASp.YFP}, Scer\GAL4^btl.PS has larval tracheal system | embryonic stage phenotype

(Tsarouhas et al., 2023)

Rab11^N124I.UAS, Rab35^S22N.UAS.YFP, Scer\GAL4^btl.PS has tracheal lumen | embryonic stage phenotype

(Tsarouhas et al., 2023)

Rab11^N124I.UAS, Rab35^S22N.UAS.YFP, Scer\GAL4^btl.PS has larval tracheal system | embryonic stage phenotype

(Tsarouhas et al., 2023)

Rab11^N124I.UAS, Scer\GAL4^GMR.PF, rpr^GMR.PH has eye phenotype

(Tiwari and Roy, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Ocrl^Δ3 mutants also expressing Rab11^{N124I.Scer\UAS} under the Scer\GAL4^He.PZ driver are early larval lethal.

(Del Signore et al., 2017)

The larval crawling defects characteristic for TBPH^Δ23/+ third instar larvae are significantly improved by expressing Rab11^{N124I.Scer\UAS} under the Scer\GAL4^VGlut.PD driver in the mutant background.

(Deshpande et al., 2016)

The reduced eye size caused by expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF is partially suppressed by bsk²/+ or msn¹⁷²/+.

The reduced eye size caused by expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF is significantly suppressed by co-expression of either bsk^{K53R.Scer\UAS} or puc^Scer\UAS.cMa.

The reduced eye phenotype caused by expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of Traf4^EP578, egr^Scer\UAS.cIa or Tak1^Scer\UAS.cTa.

The reduced eye phenotype caused by expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced if the flies also carry puc^E69/+.

(Tiwari and Roy, 2009)

Xenogenetic Interactions

Statement

Reference

The neuromuscular junction growth defects characteristic for third instar larvae expressing Hsap\TARDBP^Scer\UAS.cHa under the control of Scer\GAL4^VGlut.PD (reduced number of NMJ boutons, decreased NMJ area, length of the longest NMJ branch) as well as larval crawling defects and increased tkv-mCherry particle mobility (expressed using the tkv^{Scer\UAS.RD.T:Disc\RFP-mCherry} transgene) can be partially rescued by co-expression of Rab11^{N124I.Scer\UAS}.

(Deshpande et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Rab11^DN

(Del Signore et al., 2017, Deshpande et al., 2016)

Rab11^{N124I.Scer\UAS}

Rab11^N124I.UAS

Rab11^N124I

(Deshpande et al., 2016)

rab11^N124I

(Del Signore et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

Open Close