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General Information
Symbol
Dmel\OcrlΔ3
Species
D. melanogaster
Name
FlyBase ID
FBal0336684
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

A 2170 bp deletion resulting from the imprecise excision of P{EPgy2}OcrlEY15890 reported at X:1 ,817,066..1,819,235 in R5 coordinates.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

2167bp deletion within Ocrl.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

OcrlΔ3 larvae show a dramatic increase in the number of both circulating and sessile hemocytes (which are often multinucleate suggesting cytokinetic defects and have spikier morphology due to increased F-actin assembly) compared to controls (no obvious differences in cell proliferation are observed however) and frequently display large melanotic masses in their posterior body cavity. Notably, OcrlΔ3 larvae accumulate excess hemocytes and die at late larval/pupal stage even when raised under axenic conditions. The primary lymph gland lobes of OcrlΔ3 mutants appear partially disintegrated, while secondary lobes are intact and the larvae also exhibit aberrations in hemocyte differentiation (abnormally high proportion of hemocytes also display lamellocyte marker and most of them morphologically resemble plasmatocytes). Defects in compartment abundance, morphology or behavior throughout the endosomal system are also observed: The OcrlΔ3 mutant hemocytes exhibit reduced internalization of protein in pulse-chase with maleylated BSA (bovine serum albumin), there is also a small deficit in phagocytosis assessed by the number of internalized Escherichia coli particles. Furthermore, the LysoTracker endolysosomal marker is highly increased.

Neither the excess of circulating hemocytes nor the melanotic mass phenotype is observed in the rescue OcrlΔ3;Dp(1;3)DC402 larvae, the hemocyte differentiation defects are also rescued.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The increased number of circulating hemocytes characteristic for OcrlΔ3 mutant third instar larvae is partially suppressed by Scer\GAL4He.PZ-driven expression of either Rab11QL.Scer\UAS or Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP but not Rab5Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP. The expression of Rab11QL.Scer\UAS or Rab7T22N.Scer\UAS.P\T.T:Avic\GFP-YFP also suppresses the increase in F-actin in the mutant hemocytes as well as the expansion of the LysoTracker-positive compartments but it does not rescue the adult lethality of OcrlΔ3 animals. OcrlΔ3 mutants expressing either Rab5S43N.Scer\UAS or Rab11N124I.Scer\UAS (with the Scer\GAL4He.PZ driver) are early larval lethal.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The dramatic increase in the number of circulating hemocytes observed in OcrlΔ3 mutant larvae can be rescued by expression of OcrlScer\UAS.T:Avic\GFP driven by either Scer\GAL4He.PZ or Scer\GAL4Mef2.PU but not by any of the following: Scer\GAL4HHLT, Scer\GAL4dome-PG14, Scer\GAL4Ser.PBS, Scer\GAL4Dot.PK or Scer\GAL4Lsp2.PU.

Scer\GAL4He.PZ-driven expression of neither OcrlH469R.Scer\UAS.T:Avic\GFP nor Ocrlcat.Scer\UAS.P\T.T:Disc\RFP-mCherry.T:Atha\CRY2) can restore the elevated hemocyte numbers but the latter rescues the endocytosis defects in OcrlΔ3 mutant larvae.

Expression of OcrlScer\UAS.T:Avic\GFP with the Scer\GAL4He.PZ driver only partially rescues the hemocyte differentiation defects characteristic for OcrlΔ3 mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
OcrlΔ3
Name Synonyms
Secondary FlyBase IDs
    References (1)