H469R | Ocrl-PA; H469R | Ocrl-PB
Analogous mutation in human OCRL implicated in Lowe oculocerebrorenal syndrome; mutation carried on in vitro construct.
UAS regulatory sequences drive expression of a phosphatase-inactive form of Ocrl (carries the mutation H469R). This lesion is equivalent to a mutation associated with Lowe Syndrome in the human ortholog. The protein is tagged with GFP.
Scer\GAL4He.PZ/OcrlH469R.UAS.GFP fails to rescue OcrlΔ3
The dramatic increase in the number of circulating hemocytes observed in OcrlΔ3 mutant larvae cannot be rescued by Scer\GAL4He.PZ-driven expression of OcrlH469R.Scer\UAS.T:Avic\GFP.