FlyBase Allele Report: Dmel\ec[spok]

General Information

Symbol

Dmel\ec^spok

Species

D. melanogaster

Name

spin doktor

FlyBase ID

FBal0243618

Feature type

allele

Associated gene

Dmel\ec

Associated Insertion(s)

P{UAS-ATN1.wt}ec^spok

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Montrasio et al., 2007)

Mutagen

P-element activity

(Montrasio et al., 2007)

Progenitor genotype

Associated Insertion(s)

P{UAS-ATN1.wt}ec^spok

Cytology

Description

Insertion of a P{UAS-ATN1.wt} element just upstream of ec.

(Montrasio et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death | pupal stage P6

(Montrasio et al., 2007)

decreased cell death | pupal stage P7

(Montrasio et al., 2007)

viable

(Montrasio et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

ec^spok homozygous adult eyes display strong ommatidial displacement defects, with approximately 15% of ommatidia misoriented by more than 20 degrees with respect to the WT axis. In third instar imaginal discs, very rare orientation defects are observed, but by 24 hours after pupal formation (APF) pupal retina show robust rotation defects, disrupted sorting of the lattice, and extra interommatidial cells (including more primary pigment cells) aberrantly organised in multiple layers. This defect in programmed cell death is also evident at 42 hours APF, where an increase in lattice interommatidial cell survival is observed (~13 cells compared to 9 in wildtype), and the overall appearance of the lattice is highly disorganised.

ec^spok/Df(1)GA102 adult eyes display strong ommatidial displacement defects, with approximately 15% of ommatidia misoriented by more than 20 degrees with respect to the WT axis.

ec^spok/Df(1)HC244 adult eyes display strong ommatidial displacement defects, with approximately 15% of ommatidia misoriented by more than 20 degrees with respect to the WT axis.

ec^spok/ec¹ adult eyes display intermediate ommatidial orientation defects, reduced in frequency compared to ec^spok homozygotes, but increased compared to ec¹ homozygotes.

ec^spok/ec^KG09175 adult eyes display intermediate ommatidial orientation defects, reduced in frequency compared to ec^spok homozygotes, but increased compared to ec^KG09175 homozygotes.

ec^spok/ec^KG10228 adult eyes display intermediate ommatidial orientation defects, reduced in frequency compared to ec^spok homozygotes, but increased compared to ec^KG10228 homozygotes.

(Montrasio et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

ec^spok has ommatidium phenotype, suppressible | partially by S[+]/S^48-5

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, suppressible | partially by spi²⁵⁹/spi[+]

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, suppressible | partially by Df(3L)Ar14-8/+

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, suppressible | partially by bsk²/bsk[+]

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, suppressible | partially by shg[+]/shg¹

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, suppressible | partially by nmo^E33/nmo[+]

(Montrasio et al., 2007)

NOT suppressed by

Statement

Reference

ec^spok has ommatidium phenotype, non-suppressible by Rho1^79-3

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, non-suppressible by hep^r75

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, non-suppressible by CadN^Δ14

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, non-suppressible by Vang^x

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, non-suppressible by pk^pk-sple-13

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, non-suppressible by Egfr^t1

(Montrasio et al., 2007)

ec^spok has ommatidium phenotype, non-suppressible by aos^rlt

(Montrasio et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The ommatidial rotation defects observed in ec^spok hemizygous adult eyes are partially suppressed by bsk²/+, shg¹/+, nmo^E33/+, S^48-5/+, spi²⁵⁹/+, or Df(3L)Ar14-8/+.

The ommatidial rotation defects observed in ec^spok hemizygous adult eyes are not suppressed by Rho1^79-3/+, hep^r75/+, CadN^Δ14/+, Vang^x/+, pk^pk-sple-13/+, Egfr^t1/+, or argos^rlt/+.

(Montrasio et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ec^KG10228

(Montrasio et al., 2007)

ec^KG09175

(Montrasio et al., 2007)

ec^EP1343

(Montrasio et al., 2007)

ec¹

(Montrasio et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Excision of the insertion is accompanied by reversion of the mutant phenotype.

(Montrasio et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ec^spok

(Montrasio et al., 2007)

spok

(Montrasio et al., 2007)

Name Synonyms

spin doktor

(Montrasio et al., 2007)

Secondary FlyBase IDs

References (1)

Report Sections

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