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General Information
Symbol
Dmel\UvragHMS01357
Species
D. melanogaster
Name
FlyBase ID
FBal0257950
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a short inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
is ameliorated by bskDN.UAS.cUa
is ameliorated by Stat92EHJ
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing UvragHMS01357 under the control of Scer\GAL4GMR71G10 does not induce mushroom body gamma neuron pruning defects.

The adulthood-only expression of UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression) lead to significant increases in the thickness and in the number of cells in the adult midgut epithelium, as compared to controls; stem cells and enteroblasts are significantly more numerous, are significantly bigger, show a significant increase in mitotic index, show protrusions that extended towards the underlying muscle layer, show a strong increase in endosomes (Rab7-positive) and show a strong decrease in the number of PI3P-positive vesicles (FYVE-labelled puncta), but do not show a significant change in the number of autophagosomes/autolysosomes (Atg8a--labelled puncta), as compared to controls; the expressing stem cells and enteroblasts show signs of failure to differentiate into enterocytes (e.g. retain esg expression and Notch signaling even after becoming polyploid and fail to express the enterocyte marker Myo1A); this expression does not significantly affect the number of enteroendocrine cells in the adult midgut, as compared to controls. These adults are short lived and defecate significantly less, despite of a similar food intake, as compared to controls.

Embryos derived from females expressing UvragHMS01357 under the simultaneous control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 show a significant delay in the clearance of the paternal mitochondrial derivatives compared to wild type. Early fertilised eggs have multivesicular clusters which are largely devoid of microvesicles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
NOT Suppressor of
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The increased numbers of intestinal stem cells/enteroblast and the increased mitotic index induced in the adult midgut epithelium by the adulthood-only expression of UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression) are suppressed by the co-expression of either bskDN.UAS.cUa or Stat92EJF01265. The increased mitotic index in the adult midgut epithelium is also suppressed by heterozygosity for either Stat92E06346, Stat92EHJ, bsk1 or bsk2, but not by heterozygosity for wgl-8 or wgl-12.

The adulthood-only co-expression of UvragHMS01357 with either panΔN.UAS, Atg5JF02703 or Atg14KK100903 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to the same severe decreased numbers of intestinal stem cells and mitotic figures in the adult midgut epithelium as upon the single expression of panΔN.UAS, Atg5JF02703 or Atg14KK100903, rather than the converse increases induced by the single expression of UvragHMS01357.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
CG6116HMS01357
UvragHMS01357
Name Synonyms
Secondary FlyBase IDs
    References (6)