FlyBase Allele Report: Dmel\Atg14[KK100903]

General Information

Symbol

Dmel\Atg14^KK100903

Species

D. melanogaster

Name

FlyBase ID

FBal0230982

Feature type

allele

Associated gene

Dmel\Atg14

Associated Insertion(s)

Carried in Construct

P{KK100903}

Also Known As

Atg14 RNAi

Key Links

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Keleman et al., 2009.8.5)

in vitro construct - regulatory fusion

(Keleman et al., 2009.8.5)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{KK100903}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Atg14

Associated Sequence Features

dsRNA-KK100903

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

lethal - all die before end of P-stage, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

Phenotype Manifest In

adult midgut epithelium, with Scer\GAL4^NP5130

(Nagy et al., 2016)

embryonic/larval fat body | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

(Pircs et al., 2012)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Nagy et al., 2016)

Detailed Description

Statement

Reference

Flies expressing Atg14^KK100903 under the control of Scer\GAL4^Mef2.PU have a similar mitochondrial structure to wild-type controls.

(Xu et al., 2019)

The adulthood-only expression of Atg14^KK100903 under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to a severe decrease in the numbers of intestinal stem cells and of mitotic figures in the adult midgut epithelium, as compared to controls.

(Nagy et al., 2016)

No autolysosome formation occurs in larvae expressing Atg14^KK100903 under the control of Scer\GAL4^Cg.PA under starvation conditions.

(Bánréti et al., 2012)

Expression of Atg14^KK100903 using Scer\GAL4^Act5C.PP results in basal autophagy defects in larval fat body cell somatic clones.

(Pircs et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Atg14^KK100903, Scer\GAL4^NP5130 has decreased cell number | adult stage phenotype, non-suppressible by Uvrag^HMS01357, Scer\GAL4^NP5130

(Nagy et al., 2016)

Atg14^KK100903, Scer\GAL4^NP5130 has decreased occurrence of cell division | adult stage phenotype, non-suppressible by Uvrag^HMS01357, Scer\GAL4^NP5130

(Nagy et al., 2016)

Suppressor of

Statement

Reference

Atg14^KK100903, Scer\GAL4^NP5130 is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

Atg14^KK100903, Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Atg14^KK100903, Scer\GAL4^NP5130 has adult midgut epithelium phenotype, non-suppressible by Uvrag^HMS01357, Scer\GAL4^NP5130

(Nagy et al., 2016)

Atg14^KK100903, Scer\GAL4^NP5130 has intestinal stem cell of posterior adult midgut epithelium phenotype, non-suppressible by Uvrag^HMS01357, Scer\GAL4^NP5130

(Nagy et al., 2016)

Suppressor of

Statement

Reference

Atg14^KK100903, Scer\GAL4^NP5130 is a suppressor of adult midgut epithelium phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

Atg14^KK100903, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The adulthood-only co-expression of Uvrag^HMS01357 and Atg14^KK100903 under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to the same severely decreased numbers of intestinal stem cells and mitotic figures in the adult midgut epithelium as upon the single expression of Atg14^KK100903, rather than the converse increases induced by the single expression of Uvrag^HMS01357.

(Nagy et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v108559

P{KK100903}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atg14^KK100903

(Billes et al., 2018)

CG11877^KK100903

Name Synonyms

Secondary FlyBase IDs

References (7)

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