FlyBase Allele Report: Dmel\Rala[S25N.UAS]

General Information

Symbol

Dmel\Rala^S25N.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0101575

Feature type

allele

Associated gene

Dmel\Rala

Associated Insertion(s)

Carried in Construct

P{UAS-Rala.S25N}

Also Known As

DRal^S25N, UAS-RalA^S25N

Key Links

Transgenic product class

dominant_negative_variant

(Sawamoto et al., 1999)

missense_variant

(Sawamoto et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Sawamoto et al., 1999)

missense_variant

(Sawamoto et al., 1999)

Mutagen

in vitro construct

(Sawamoto et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Rala.S25N}

Cytology

Description

A dominant negative form of Rala (carries the amino acid replacement S25N) is expressed under the control of UASt regulatory sequences.

(Sawamoto et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rala

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 4

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Kankel et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^elav-C155

(Richhariya et al., 2017)

decreased size | adult stage, with Scer\GAL4^Bx-MS1096

(Brown et al., 2024)

visible, with Scer\GAL4^69B

(Sawamoto et al., 1999)

visible, with Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

visible | adult stage, with Scer\GAL4^Bx-MS1096

(Brown et al., 2024)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6, Scer\GAL4^upd1.PU

(Ghiglione et al., 2008)

crossvein | increased number

(Blanke and Jackle, 2006)

scutum & macrochaeta, with Scer\GAL4^69B

(Sawamoto et al., 1999)

scutum & macrochaeta, with Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

scutum & macrochaeta & tormogen cell, with Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

scutum & macrochaeta & trichogen cell, with Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

scutum & microchaeta, with Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

scutum & microchaeta & tormogen cell, with Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

scutum & microchaeta & trichogen cell, with Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

scutum & trichome, with Scer\GAL4^69B

(Sawamoto et al., 1999)

wing, with Scer\GAL4^Bx-MS1096

(Brown et al., 2024)

wing hair, with Scer\GAL4^69B

(Sawamoto et al., 1999)

Detailed Description

Statement

Reference

Expression of Rala^{S25N.Scer\UAS} under the control of Scer\GAL4^elav-C155 leads to significant decrease in flight ability in adults (measured as flight time in a single flight assay on tethered flies), but their gross wing morphology as well as climbing ability is unaffected as compared with controls. Similar flight deficit is observed also when the Rala^{S25N.Scer\UAS} expression is limited to pupal stages (using tub-Gal80[ts] to exert the temporal control) but not if it is restricted to larval stages or to adulthood.

(Richhariya et al., 2017)

Expression of Rala^{S25N.Scer\UAS} under the control of both Scer\GAL4^slbo.2.6 and Scer\GAL4^upd blocks border cell migration in over 70% of stage-10 oocytes.

(Ghiglione et al., 2008)

Scer\GAL4^en-e16E-mediated expression of Rala^{S25N.Scer\UAS} causes wing phenotypes including duplications of crossvein starting points that fail to link to longitudinal veins.

(Blanke and Jackle, 2006)

The wing cells of flies expressing Rala^{S25N.Scer\UAS} under the control of Scer\GAL4^69B often form multiple wing hairs. The hairs are shorter than wild type and some are forked, curved or twisted. In pupal wings, cells with 2 or 3 prehairs are occasionally seen (only one prehair forms per cell in wild-type wings). The morphology of the prehairs is irregular. Hairs on the notum are also often shortened, forked, twisted, duplicated or triplicated. Some bristles on the notum are lost. Flies expressing Rala^{S25N.Scer\UAS} under the control of Scer\GAL4^sca-537.4 show loss of bristles (both macrochaetae and microchaetae) on the notum. In some cases both shafts and sockets are affected.

(Sawamoto et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^Bx-MS1096 has decreased size | adult stage phenotype, enhanceable by mnb^NIG.7826R, Scer\GAL4^Bx-MS1096

(Brown et al., 2024)

Rala^S25N.UAS, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, enhanceable by mnb^NIG.7826R, Scer\GAL4^Bx-MS1096

(Brown et al., 2024)

NOT Enhanced by

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Df(3L)Ar14-8

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Df(3L)pbl-X1

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Dsor1^Su1

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by rl⁹

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by rl^Su23

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Raf¹

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by mbt^P1

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by mbt^P2

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Cdc42¹

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Rho1^Df236

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Rho1^Df903

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Rho1^k07903

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Ras85D^e2F

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Dsor1^Gp158

(Sawamoto et al., 1999)

NOT suppressed by

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Df(3L)Ar14-8

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Df(3L)pbl-X1

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Dsor1^Gp158

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Dsor1^Su1

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by rl⁹

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by rl^Su23

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by mbt^P1

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by mbt^P2

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Cdc42¹

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Rho1^Df236

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Rho1^Df903

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Rho1^k07903

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Ras85D^e2F

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Raf¹

(Sawamoto et al., 1999)

Enhancer of

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^Bx-MS1096 is an enhancer of visible | adult stage phenotype of Scer\GAL4^Bx-MS1096, mnb^NIG.7826R

(Brown et al., 2024)

Rala^S25N.UAS, Scer\GAL4^Bx-MS1096 is an enhancer of decreased size | adult stage phenotype of Scer\GAL4^Bx-MS1096, mnb^NIG.7826R

(Brown et al., 2024)

Rala^S25N.UAS, Scer\GAL4^C855a is an enhancer of decreased size | larval stage phenotype of Scer\GAL4^C855a, mnb^NIG.7826R

(Brown et al., 2024)

Suppressor of

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^30.UAS.EGFP

(Kankel et al., 2020)

NOT Suppressor of

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\TARDBP^{M337V.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Kankel et al., 2020)

Rala^S25N.UAS, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\FUS^{R521C.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Kankel et al., 2020)

Other

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^da.G32, mnb^NIG.7826R has lethal phenotype

(Brown et al., 2024)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by mnb^NIG.7826R, Scer\GAL4^Bx-MS1096

(Brown et al., 2024)

Suppressed by

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has scutum & macrochaeta phenotype, suppressible by hep[+]/hep^r75

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has scutum & macrochaeta phenotype, suppressible by hep[+]/hep¹

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has scutum & macrochaeta phenotype, suppressible by bsk^flp147E/bsk[+]

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has scutum & macrochaeta phenotype, suppressible by bsk²/bsk[+]

(Sawamoto et al., 1999)

Rala^S25N.UAS, Scer\GAL4^sca-537.4 has scutum & macrochaeta phenotype, suppressible by bsk¹/bsk[+]

(Sawamoto et al., 1999)

Enhancer of

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, mnb^NIG.7826R

(Brown et al., 2024)

Rala^S25N.UAS, Scer\GAL4^C855a is an enhancer of larval brain phenotype of Scer\GAL4^C855a, mnb^NIG.7826R

(Brown et al., 2024)

Suppressor of

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^30.UAS.EGFP

(Kankel et al., 2020)

Rala^S25N.UAS, Scer\GAL4^ap-md544 is a suppressor of wing hair | increased number phenotype of Scer\GAL4^ap-md544, trc^T453A.UAS.L

(He et al., 2005)

NOT Suppressor of

Statement

Reference

Rala^S25N.UAS, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\TARDBP^{M337V.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Kankel et al., 2020)

Rala^S25N.UAS, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\FUS^{R521C.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Kankel et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of the dominant negative Rala^{S25N.Scer\UAS} with trc^{T453A.Scer\UAS}, under the control of Scer\GAL4^ap-md544, leads to a complete suppression of the trc^{T453A.Scer\UAS} phenotype. In contrast, coexpression of the constitutively active Rala^{G20V.Scer\UAS} transgene with trc^{T453A.Scer\UAS}, under the control of Scer\GAL4^ap-md544, results in a dramatic enhancement of the multiple wing hair phenotype.

(He et al., 2005)

The bristle loss phenotype caused by expression of Rala^{S25N.Scer\UAS} under the control of Scer\GAL4^sca-537.4 is dominantly suppressed by hep^r75, hep¹, bsk^flp147E, bsk² or bsk¹.

(Sawamoto et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rala^S25N.UAS is rescued by Rala^UAS.cSa/Scer\GAL4^69B

(Sawamoto et al., 1999)

Fails to rescue

Rala^S25N.UAS/Scer\GAL4^Rala-PG69 fails to rescue Rala^PG69

(Ghiglione et al., 2008)

Comments

Expression of Rala^{S25N.Scer\UAS} under the control of Scer\GAL4^Rala-PG69 in a Rala^PG69 mutant background does not rescue lethality and border cell migration.

(Ghiglione et al., 2008)

The loss of bristles and morphological defects on the notum caused by Rala^{S25N.Scer\UAS},Scer\GAL4^69B flies, are largely rescued by overexpression of Rala^Scer\UAS.cSa.

(Sawamoto et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

32094

w^*; P{UAS-Rala.S25N}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DRal^S25N

(Sawamoto et al., 1999)

Dral^S25N

(He et al., 2005)

Rala^{S25N.Scer\UAS}

Rala^S25N.UAS

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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