FlyBase Allele Report: Dmel\Shark[UAS.cFa]

General Information

Symbol

Dmel\Shark^UAS.cFa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Fernandez

FlyBase ID

FBal0104428

Feature type

allele

Associated gene

Dmel\Shark

Associated Insertion(s)

Carried in Construct

P{UAS-Shark.F}

Also Known As

UAS-shark

Key Links

Transgenic product class

wild_type

(Fernandez et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Fernandez et al., 2000)

Mutagen

in vitro construct

(Fernandez et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Shark.F}

Cytology

Description

UASt regulatory sequences drive expression of the Shark open reading frame devoid of all UTR sequences.

(Fernandez et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Shark

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates synucleinopathy

modeled by Hsap\SNCA^UAS.cFa

(Chen et al., 2009)

modeled by Hsap\SNCA^S129D.UAS

(Chen et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Shark^UAS.cFa, Scer\GAL4^elav.PU is a suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\SNCA^S129D.UAS, Scer\GAL4^elav.PU

(Chen et al., 2009)

Shark^UAS.cFa, Scer\GAL4^elav.PU is a suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav.PU

(Chen et al., 2009)

NOT Suppressor of

Statement

Reference

Shark^UAS.cFa, Scer\GAL4^elav.PU is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\SNCA^YF.UAS, Scer\GAL4^elav.PU

(Chen et al., 2009)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^CY2/Shark^UAS.cFa is a non-enhancer of dorsal appendage phenotype of bwk¹⁵¹, cic⁰⁸⁴⁸²

(Tran and Berg, 2003)

Suppressor of

Statement

Reference

Shark^UAS.cFa, Scer\GAL4^elav.PU is a suppressor of dopaminergic neuron | adult stage phenotype of Hsap\SNCA^S129D.UAS, Scer\GAL4^elav.PU

(Chen et al., 2009)

Shark^UAS.cFa, Scer\GAL4^GMR.PU is a suppressor of ommatidium | adult stage phenotype of Hsap\SNCA^S129D.UAS, Scer\GAL4^GMR.PU

(Chen et al., 2009)

Shark^UAS.cFa, Scer\GAL4^elav.PU is a suppressor of dopaminergic neuron | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav.PU

(Chen et al., 2009)

Scer\GAL4^c415/Shark^UAS.cFa is a suppressor | partially of dorsal appendage phenotype of bwk¹⁵¹, cic⁰⁸⁴⁸²

(Tran and Berg, 2003)

Scer\GAL4^55B/Shark^UAS.cFa is a suppressor | partially of dorsal appendage phenotype of bwk¹⁵¹, cic⁰⁸⁴⁸²

(Tran and Berg, 2003)

NOT Suppressor of

Statement

Reference

Shark^UAS.cFa, Scer\GAL4^elav.PU is a non-suppressor of dopaminergic neuron | adult stage phenotype of Hsap\SNCA^YF.UAS, Scer\GAL4^elav.PU

(Chen et al., 2009)

Scer\GAL4^CY2/Shark^UAS.cFa is a non-suppressor of dorsal appendage phenotype of bwk¹⁵¹, cic⁰⁸⁴⁸²

(Tran and Berg, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The mutant dorsal appendage phenotype seen in bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs is partially suppressed by the expression of shark^Scer\UAS.cFa under the control of Scer\GAL4^c415 or Scer\GAL4^55B. Expression of shark^Scer\UAS.cFa under the control of Scer\GAL4^CY2 has little effect on the mutant dorsal appendage phenotype seen in bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs.

(Tran and Berg, 2003)

Xenogenetic Interactions

Statement

Reference

Coexpression of shark^Scer\UAS.cFa significantly rescues the neurotoxicity of Scer\GAL4^elav.PU-mediated Hsap\SNCA^Scer\UAS.cFa expression.

Coexpression of shark^Scer\UAS.cFa significantly rescues the neurotoxicity of Scer\GAL4^elav.PU-mediated Hsap\SNCA^{S129D.Scer\UAS} expression.

Coexpression of shark^Scer\UAS.cFa does not significantly rescue the neurotoxicity of Scer\GAL4^elav.PU-mediated Hsap\SNCA^YF.Scer\UAS expression.

Coexpression of shark^Scer\UAS.cFa attenuates the retinal degeneration caused by Scer\GAL4^GMR.PU-mediated Hsap\SNCA^{S129D.Scer\UAS} expression.

(Chen et al., 2009)

Complementation and Rescue Data

Rescues

Shark^UAS.cFa/Scer\GAL4^69B rescues Shark¹

(Fernandez et al., 2000)

Fails to rescue

Scer\GAL4^pnr-MD237/Shark^UAS.cFa fails to rescue Shark¹

(Fernandez et al., 2000)

Comments

Rescues the dorsal closure defect of shark¹ embryos derived from shark¹ female germline clones when expressed under the control of Scer\GAL4^69B. Fails to rescue the dorsal closure defect of shark¹ embryos derived from shark¹ female germline clones when expressed under the control of Scer\GAL4^pnr-MD237.

(Fernandez et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59001

w^*; P{UAS-Shark.F}2, Shark¹/SM6b, P{eve-lacZ8.0}SB1

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2 cells to confirm functionality of the shark^Scer\UAS.cFa product.

(Fernandez et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Shark^Scer\UAS.cFa

Shark^UAS.cFa

shark^Scer\UAS.cFa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Fernandez

(Fernandez et al., 2000)

Secondary FlyBase IDs

References (3)

Report Sections

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