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General Information
Symbol
Dmel\eyaUAS.cHa
Species
D. melanogaster
Name
UAS construct of Hsiao
FlyBase ID
FBal0150506
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-eyaWT
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of the eya coding region is under the control of UASt sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The average penetrance of ectopic eye induction in eyaScer\UAS.cHa; Scer\GAL4dpp.blk1 flies is 49% (over 8 independent P{UAS-eya.H} insertions). Ectopic eyes are typically seen on the head, either close to the eyes and near the base of the antennae. Patches of red pigement are also frequently seen in the wing hinges and on the underside of metathoracic legs, and ocassionally elsewhere on wings legs and antennae. The penetrance of ectopic eye induction in eyaScer\UAS.cHa; Scer\GAL4dpp.blk1 flies is reduced from 98% (n=298) to 50% (n=313) by heterozygosity for eyaG130 (data from a single, 'strong' P{UAS-eya.H} insertion). eyaScer\UAS.cHa; Scer\GAL4dpp.PH flies have highly variable wing defects, including ectopic bristles on L3 and in more extreme cases, blistering, and defects in overall shape: Proportion with blistered wings = 40%; proportion with only mild wing defects = 60%. These flies also have increased numbers of scutellar machrochaete (average= 6.0, compared to 4 in wild-type), and problems with thoracic closure. They also have a low penetrance of rough eyes, and arista to leg transformations. Note: all of the above data is an average result from 8 independent P{UAS-eya.H} insertions. Heterozygosity for eyaG130 leads to a decrease in the proportion of eyaScer\UAS.cHa; Scer\GAL4dpp.PH flies with blistered wings (76% to 3%) and an increase in the proportion with only milder wing defects (24% to 97%) (data from a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference

Scer\GAL4dpp.PH, eyaUAS.cHa has wing phenotype, non-enhanceable by bsk1

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Scer\GAL4dpp.blk1, eyaUAS.cHa has eye | ectopic phenotype, non-suppressible by bsk1

Scer\GAL4dpp.PH, eyaUAS.cHa has wing phenotype, non-suppressible by bsk1

Additional Comments
Genetic Interactions
Statement
Reference

The penetrance of ectopic eye induction in eyaUAS.cHa; Scer\GAL4dpp.blk1 flies is reduced from 98% (n=298) to 50% (n=313) by heterozygosity for rlS-135 and to 58% (n=313), by heterozygosity for Egfrunspecified (data from a single, 'strong' P{UAS-eya.H} insertion). No suppression of penetrance was seen due to heterozygosity for bsk1 or the presence of rlSem.cUa.UAS, or bskUAS.cBa. Ectopic eyes in eyaUAS.cHa; Scer\GAL4dpp.blk1; rlSem.cUa.UAS are generally larger, and have more ommatidial definition than those in eyaUAS.cHa; Scer\GAL4dpp.blk1 flies. The proportion of eyaUAS.cHa; Scer\GAL4dpp.PH flies with blistered wings (76%) is decreased by heterozygosity for dac3 (to 4%), so3 (to 17%), rlS-135 (to 10%), or Egfrunspecified (to 14%), increased by heterozygosity for rlSem.cUa.UAS (to 93%), and relatively unaffected by heterozygosity for bsk1 (69%), or the presence of bskUAS.cBa (82%). Conversely, the proportion of eyaUAS.cHa; Scer\GAL4dpp.PH flies with only mild wing defects (24%) is increased by heterozygosity for dac3 (to 96%), so3 (to 83%), rlS-135 (to 90%), or Egfrunspecified (to 86%) decreased by heterozygosity for rlSem.cUa.UAS (to 7%), and relatively unaffected by heterozygosity for bsk1 (31%), or the presence of bskUAS.cBa (18%). (All data from experiments with a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

eyaScer\UAS.cHa; Scer\GAL4ey.PB partially rescues the loss of eye phenotype of eya2 mutant flies.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
eyaScer\UAS.cHa
eyaUAS.cHa
Name Synonyms
UAS construct of Hsiao
Secondary FlyBase IDs
    References (4)