FlyBase Allele Report: Dmel\Hnf4[UAS.ORF.GW.Tag:HA]

General Information

Symbol

Dmel\Hnf4^{UAS.ORF.GW.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0297051

Feature type

allele

Associated gene

Dmel\Hnf4

Associated Insertion(s)

Carried in Construct

M{UAS-Hnf4.ORF.3xHA.GW}

Key Links

Transgenic product class

wild_type

(Bischof and FlyORF project members, 2014.6.20)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Bischof and FlyORF project members, 2014.6.20)

Mutagen

in vitro construct

(Bischof and FlyORF project members, 2014.6.20)

Progenitor genotype

Carried in construct

M{UAS-Hnf4.ORF.3xHA.GW}

Cytology

Description

UAS regulatory sequences drive expression of a full-length Hnf4 open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Bischof and FlyORF project members, 2014.6.20)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Also carries

recombinase target site

FRT5

recombinase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | third instar larval stage, with Scer\GAL4^sns.GCN

(Marchesin et al., 2019)

decreased cell number | third instar larval stage, with Scer\GAL4^Ugt36A1.PK

(Marchesin et al., 2019)

decreased cell size | third instar larval stage, with Scer\GAL4^sns.GCN

(Marchesin et al., 2019)

decreased cell size | third instar larval stage, with Scer\GAL4^Ugt36A1.PK

(Marchesin et al., 2019)

lethal | male limited, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

lethal | male limited | cold sensitive, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

partially lethal, with Scer\GAL4^Ugt36A1.PK

(Marchesin et al., 2019)

partially lethal | female limited, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

partially lethal | female limited | cold sensitive, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

some die during third instar larval stage, with Scer\GAL4^Ugt36A1.PK

(Marchesin et al., 2019)

viable, with Scer\GAL4^sns.GCN

(Marchesin et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Third instar larval garland nephrocytes expressing Hnf4^{UAS.ORF.GW.Tag:HA} under the control of Scer\GAL4^Ugt36A1.PK exhibit a severe decrease in lipid droplet content (BODIPY staining) when expressing at low levels (18[o]C), or exhibit a strong lipid droplet accumulation when expressing at higher levels (29[o]C). Unlike controls, there is abundant cytoplasmic phagophores and autophagosomes, whose limiting membranes are often multilamellar in structure; unlike the perinuclear compact ER decorated with ribosomes observed in controls, ER structures are difficult to detect despite their abnormal expansion.

High expression of Hnf4^{UAS.ORF.GW.Tag:HA} under the control of either Scer\GAL4^Ugt36A1.PK or Scer\GAL4^sns.GCN causes a reduction in the size and number of third instar larval nephrocytes, as compared to controls.

(Marchesin et al., 2019)

Expression of Hnf4^{Scer\UAS.ORF.GW.T:Ivir\HA1} under the control of Scer\GAL4^Bx-MS1096 results in partial female lethality at 18[o]C. The adult flies that are seen exhibit almost complete wing loss at 18[o]C.

(Schertel et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hnf4^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^Desat1.PB is a suppressor | partially of abnormal courtship behavior phenotype of Scer\GAL4^Desat1.PB, fru^KK112763

(Sun et al., 2023)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hnf4^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^Desat1.PB is a suppressor | partially of lipid droplet | increased number | adult stage phenotype of Scer\GAL4^Desat1.PB, fru^KK112763

(Sun et al., 2023)

NOT Suppressor of

Statement

Reference

Hnf4^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^Ugt36A1.PK is a non-suppressor of lipid droplet | third instar larval stage phenotype of Scer\GAL4^Ugt36A1.PK, mdy^HMC06242

(Marchesin et al., 2019)

Hnf4^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^Ugt36A1.PK is a non-suppressor of embryonic/larval garland cell | third instar larval stage phenotype of Scer\GAL4^Ugt36A1.PK, mdy^HMC06242

(Marchesin et al., 2019)

Other

Statement

Reference

Hnf4^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^Ugt36A1.PK, mdy^HMC06242 has embryonic/larval garland cell | third instar larval stage phenotype

(Marchesin et al., 2019)

Hnf4^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^Ugt36A1.PK, mdy^HMC06242 has lipid droplet | third instar larval stage phenotype

(Marchesin et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing mdy^HMC06242 and Hnf4^{UAS.ORF.GW.Tag:HA} under the control of Scer\GAL4^Ugt36A1.PK abolishes lipid droplets in third instar larval garland nephrocytes in both high (29[o]C) and low (18[o]C) Hnf4^{UAS.ORF.GW.Tag:HA} expressing conditions.

(Marchesin et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

FlyORF

F000144

M{UAS-Hnf4.ORF.3xHA.GW}ZH-86Fb

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hnf4^{Scer\UAS.ORF.GW.T:Ivir\HA1}

Hnf4^{UAS.ORF.GW.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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