FlyBase Allele Report: Dmel\NLaz[NW5]

General Information

Symbol

Dmel\NLaz^NW5

Species

D. melanogaster

Name

FlyBase ID

FBal0239917

Feature type

allele

Associated gene

Dmel\NLaz

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

ends-in gene targeting

(Ganfornina, 2024.4.29)

recombination

(Hull-Thompson et al., 2009)

I-SceI

(Hull-Thompson et al., 2009)

gene targeting by homologous recombination

(Hull-Thompson et al., 2009)

Progenitor genotype

Cytology

Description

Knock-out allele.

(Hull-Thompson et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior

(Ruiz et al., 2012)

abnormal courtship behavior | male

(Ruiz et al., 2014)

abnormal starvation stress response

(Ruiz et al., 2012)

abnormal stress response

(Hull-Thompson et al., 2009)

chemical sensitive

(Ruiz et al., 2012)

short lived

(Hull-Thompson et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous males have a significantly reduced courtship index compared to controls.

(Ruiz et al., 2014)

Homozygous flies are more sensitive to starvation-desiccation and to iron toxicity (treated with FeSO[[4]]) than wild-type controls.

(Ruiz et al., 2012)

Homozygotes show reduced energy stores (lipids and carbohydrates) under non-starvation conditions compared to control flies. The mutant flies show rapid depletion of nutrient stores (lipids and carbohydrates) upon wet starvation compared to control flies.

Homozygotes are significantly more sensitive to dry starvation than wild-type controls.

Homozygotes show increased sensitivity to paraquat compared to controls.

Homozygotes are short lived compared to controls.

(Hull-Thompson et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

NLaz^NW5 has abnormal starvation stress response phenotype, suppressible by Same\Laz^UAS.cRa/Scer\GAL4^da.G32

(Ruiz et al., 2012)

NLaz^NW5 has chemical sensitive phenotype, suppressible by Same\Laz^UAS.cRa/Scer\GAL4^da.G32

(Ruiz et al., 2012)

NOT suppressed by

Statement

Reference

NLaz^NW5 has abnormal courtship behavior phenotype, non-suppressible by Same\Laz^UAS.cRa/Scer\GAL4^da.G32

(Ruiz et al., 2012)

Suppressor of

Statement

Reference

NLaz[+]/NLaz^NW5 is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of sicily^E

(Liu et al., 2017)

NOT Suppressor of

Statement

Reference

NLaz^NW5 is a non-suppressor of abnormal developmental rate phenotype of BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544

(Romão et al., 2021)

Phenotype Manifest In

Suppressor of

Statement

Reference

NLaz[+]/NLaz^NW5 is a suppressor of photoreceptor | somatic clone | adult stage | progressive phenotype of sicily^E

(Liu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The photoreceptor degeneration observed in adult flies bearing ey>flip whole-eye clones mutant for sicily^E is improved by NLaz^NW5 heterozygosity.

(Liu et al., 2017)

The increased tolerance of animals expressing hep^Scer\UAS.cBa under the control of Scer\GAL4^ppl.PP to paraquat is abolished by NLaz^NW5/NLaz^NW5, with the double mutant flies showing the same sensitivity to paraquat as NLaz^NW5/NLaz^NW5 single mutants.

(Hull-Thompson et al., 2009)

Xenogenetic Interactions

Statement

Reference

The increased sensitivity to starvation-desiccation and to iron toxicity (treated with FeSO[[4]]) seen in NLaz^NW5 homozygotes is rescued by expression of Same\Laz^Scer\UAS.cRa under the control of Scer\GAL4^da.G32. However, the courtship defects seen in NLaz^NW5 homozygotes are not rescued.

(Ruiz et al., 2012)

Complementation and Rescue Data

Rescued by

NLaz^NW5 is rescued by Scer\GAL4^da.PU/NLaz^UAS.cRa

(Ruiz et al., 2014)

NLaz^NW5 is rescued by Scer\GAL4^da.PU/NLaz^N165S.UAS

(Ruiz et al., 2014)

Partially rescued by

NLaz^NW5 is partially rescued by Scer\GAL4^da.PU/NLaz^L130R.UAS

(Ruiz et al., 2014)

NLaz^NW5 is partially rescued by Scer\GAL4^da.PU/NLaz^{Δ177-199.UAS}

(Ruiz et al., 2014)

Comments

Expression of NLaz^Scer\UAS.cRa under the control of Scer\GAL4^da.PU rescues the reduced longevity of NLaz^NW5 flies seen in the absence of added stress and also rescues survival of 3 day old NLaz^NW5 flies exposed to paraquat or to starvation-dessication stress.

Expression of NLaz^{L130R.Scer\UAS} under the control of Scer\GAL4^da.PU partially rescues survival of 3 day old NLaz^NW5 flies exposed to paraquat or to starvation-dessication stress, but is unable to rescue the reduced longevity of NLaz^NW5 flies seen in the absence of added stress.

Expression of NLaz^{Δ177-199.Scer\UAS} under the control of Scer\GAL4^da.PU partially rescues median survival, but fully rescues maximal survival of 3 day old NLaz^NW5 flies exposed to paraquat.

Expression of NLaz^{N165S.Scer\UAS} under the control of Scer\GAL4^da.PU results in extended survival of 3 day old NLaz^NW5 flies exposed to paraquat.

Expression of NLaz^Scer\UAS.cRa under the control of Scer\GAL4^da.PU rescues the reduced courtship index of NLaz^NW5 males.

(Ruiz et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

603625

w^*; NLaz^NW5

600610

w^*; NLaz^NW5 GLaz^Δ2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

NLaz^NW5

(Ganfornina, 2024.4.29, Romão et al., 2021, Ruiz et al., 2012, Hull-Thompson et al., 2009)

NW5

(Ruiz et al., 2014)

Nlaz^NW5

(Liu et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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