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General Information
Symbol
Dmel\unc-104GD13526
Species
D. melanogaster
Name
FlyBase ID
FBal0210831
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
unc-104 RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing unc-104GD13526 in Insulin Producing Cells (IPCs) under the control of Scer\GAL4Ilp2.PW dramatically reduces adult size. Expression of unc-104GD13526 also reduces the number of IPCs, disrupts IPC morphology, and imposes ~1 day of developmental delay in eclosion. Limiting unc-104GD13526 expression to late larval development onwards using Scer\GAL80ts.αTub84B prevents occurrence of any visible defects in IPC development.

Larval ddaE neurons expressing unc-104GD13526 under the control of Scer\GAL4221 (and Dicer-2, for a more efficient RNAi) show a significant increase in growing microtubules within dendrites, as compared to neurons in controls. 12h after axotomy, these neurons show significantly delayed axon beading, as compared to similarly injured control neurons.

Larval ddaC neurons expressing unc-104GD13526 under the control of Scer\GAL4477 (and Dicer-2, for a more efficient RNAi) show a moderately reduced dendritic arborization which does not expand as the larval life progresses, as compared to controls. 12h after axotomy, these neurons show a significant increase in growing microtubules within axons, as compared to similarly injured control neurons.

Adults expressing unc-104GD13526 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Unc104<up>RNAi GD</up>
unc-104GD13526
Name Synonyms
Secondary FlyBase IDs
    References (11)