FlyBase Allele Report: Dmel\unc-104[GD13526]

General Information

Symbol

Dmel\unc-104^GD13526

Species

D. melanogaster

Name

FlyBase ID

FBal0210831

Feature type

allele

Associated gene

Dmel\unc-104

Associated Insertion(s)

Carried in Construct

P{GD13526}

Also Known As

unc-104 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD13526}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD13526

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of nervous system disease

CEA

(Li et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of nervous system disease

is ameliorated by wnd³

(Li et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size, with Scer\GAL4^Ilp2.PW

(Cao et al., 2014)

abnormal developmental rate, with Scer\GAL4^Ilp2.PW

(Cao et al., 2014)

abnormal neuroanatomy | larval stage, with Scer\GAL4^Ilp2.PW

(Cao et al., 2014)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing unc-104^GD13526 in Insulin Producing Cells (IPCs) under the control of Scer\GAL4^Ilp2.PW dramatically reduces adult size. Expression of unc-104^GD13526 also reduces the number of IPCs, disrupts IPC morphology, and imposes ~1 day of developmental delay in eclosion. Limiting unc-104^GD13526 expression to late larval development onwards using Scer\GAL80^ts.αTub84B prevents occurrence of any visible defects in IPC development.

(Cao et al., 2014)

Larval ddaE neurons expressing unc-104^GD13526 under the control of Scer\GAL4²²¹ (and Dicer-2, for a more efficient RNAi) show a significant increase in growing microtubules within dendrites, as compared to neurons in controls. 12h after axotomy, these neurons show significantly delayed axon beading, as compared to similarly injured control neurons.

Larval ddaC neurons expressing unc-104^GD13526 under the control of Scer\GAL4⁴⁷⁷ (and Dicer-2, for a more efficient RNAi) show a moderately reduced dendritic arborization which does not expand as the larval life progresses, as compared to controls. 12h after axotomy, these neurons show a significant increase in growing microtubules within axons, as compared to similarly injured control neurons.

(Chen et al., 2012)

Adults expressing unc-104^GD13526 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development