FlyBase Allele Report: Dmel\γTub23C[KK107500]

General Information

Symbol

Dmel\γTub23C^KK107500

Species

D. melanogaster

Name

FlyBase ID

FBal0234969

Feature type

allele

Associated gene

Dmel\γTub23C

Associated Insertion(s)

Carried in Construct

P{KK107500}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK107500}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK107500

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dendrite | larval stage | conditional, with Scer\GAL4^109(2)80

(Chen et al., 2012)

dendrite | larval stage | conditional, with Scer\GAL4²²¹

(Chen et al., 2012)

larval dorsal multidendritic neuron ddaE | larval stage | conditional, with Scer\GAL4^109(2)80

(Chen et al., 2012)

larval dorsal multidendritic neuron ddaE | larval stage | conditional, with Scer\GAL4²²¹

(Chen et al., 2012)

microtubule | larval stage | conditional, with Scer\GAL4^109(2)80

(Chen et al., 2012)

microtubule | larval stage | conditional, with Scer\GAL4²²¹

(Chen et al., 2012)

Detailed Description

Statement

Reference

Larval ddaE neurons expressing γTub23C^KK107500 under the control of Scer\GAL4²²¹ (and Dicer-2, for a more efficient RNAi) show decreased growing microtubules within dendrites 8h after axotomy, but do not show changes in axon regeneration at 48h upon proximal axotomy, as compared to similarly injured control neurons.

Larval ddaE neurons expressing γTub23C^KK107500 under the control of Scer\GAL4^109(2)80 (and Dicer-2, for a more efficient RNAi) show decreased growing microtubules within dendrites 24h after axotomy, as compared to similarly injured control neurons; uninjured neurons, however, do not show changes in growing microtubules within dendrites, as compared to controls.

Larval ddaE neurons expressing γTub23C^KK107500 under the control of either Scer\GAL4²²¹ or Scer\GAL4^109(2)80 (and Dicer-2, for a more efficient RNAi) do not show a decrease in the proportion of dendrites persisting 18h after consecutive axotomy and dendrite severance (i.e. axotomy-8h-dendrite severance-18h), as compared to controls.

Larval ddaC neurons expressing γTub23C^KK107500 under the control of Scer\GAL4⁴⁷⁷ (and Dicer-2, for a more efficient RNAi) show the expected complex dendrite arbors that increases in branching during larval life.

(Chen et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

γTub23C^KK107500, Scer\GAL4⁴⁷⁷ is an enhancer of larval dorsal multidendritic neuron ddaC | larval stage phenotype of Scer\GAL4⁴⁷⁷, unc-104^GD13526

(Chen et al., 2012)