FlyBase Gene Report: Dmel\unc-104

General Information

Symbol

Dmel\unc-104

Species

D. melanogaster

Name

uncoordinated-104

Annotation Symbol

CG8566

Feature Type

protein_coding_gene

FlyBase ID

FBgn0267002

Gene Model Status

Current

Stock Availability

23 publicly available

Enzyme Name (EC)

plus-end-directed kinesin ATPase (5.6.1.3)

Gene Summary

Required for presynaptic maturation, has a role in axonal transport of dense-core vesicles carrying synaptic vesicle precursors, components required for the morphological transformation of axonal growth cones to mature boutons. (UniProt, A1ZAJ2)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

imac, Klp53D, Unc104, Kif1a, DmKlp53D

Key Links

Genomic Location

Cytogenetic map

53D6-53D8

Sequence location

2R:16,751,503..16,772,671 [-]

Recombination map

2-80

RefSeq locus

NT_033778 REGION:16751503..16772671

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (31 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables GTP-dependent protein binding

inferred from physical interaction with UniProtKB:Q9VHV5

(Vukoja et al., 2018)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

enables microtubule binding

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001752

(InterPro Project Members, 2004-)

enables microtubule motor activity

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821

(InterPro Project Members, 2004-)

enables plus-end-directed microtubule motor activity

inferred from biological aspect of ancestor with PANTHER:PTN000648655

(GO Reference Genome Project, 2011-)

Biological Process (18 terms)

Terms Based on Experimental Evidence (16 terms)

CV Term

Evidence

References

involved_in anterograde axonal transport

inferred from mutant phenotype

(Cao et al., 2014)

inferred from mutant phenotype

(Vukoja et al., 2018)

involved_in anterograde synaptic vesicle transport

inferred from mutant phenotype

(Barkus et al., 2008)

involved_in axo-dendritic transport

inferred from mutant phenotype

(Kern et al., 2013)

involved_in axonal transport

inferred from mutant phenotype

(Shaw et al., 2019)

involved_in filopodium assembly

inferred from mutant phenotype

(Medina et al., 2006)

involved_in larval locomotory behavior

inferred from mutant phenotype

(Barkus et al., 2008)

involved_in negative regulation of synaptic assembly at neuromuscular junction

inferred from mutant phenotype

(Kern et al., 2013)

involved_in neuromuscular junction development

inferred from mutant phenotype

(Barkus et al., 2008)

involved_in positive regulation of insulin secretion

inferred from mutant phenotype

(Cao et al., 2014)

involved_in regulation of dendrite morphogenesis

inferred from mutant phenotype

(Kern et al., 2013)

involved_in regulation of locomotion

inferred from mutant phenotype

(Kern et al., 2013)

involved_in regulation of synapse assembly

inferred from mutant phenotype

(Kern et al., 2013)

involved_in synaptic vesicle maturation

inferred from mutant phenotype

(Pack-Chung et al., 2007)

involved_in synaptic vesicle transport

inferred from mutant phenotype

(Pack-Chung et al., 2007)

involved_in vesicle transport along microtubule

inferred from mutant phenotype

(Pack-Chung et al., 2007)

involved_in vesicle-mediated transport

inferred from mutant phenotype

(Barkus et al., 2008)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in microtubule-based movement

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821

(InterPro Project Members, 2004-)

involved_in retrograde neuronal dense core vesicle transport

inferred from biological aspect of ancestor with PANTHER:PTN000648655

(GO Reference Genome Project, 2011-)

involved_in vesicle-mediated transport

inferred from biological aspect of ancestor with PANTHER:PTN000648655

(GO Reference Genome Project, 2011-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in axon terminus

inferred from direct assay

(Shaw et al., 2019)

located_in axon

inferred from direct assay

(Cao et al., 2014)

inferred from direct assay

(Vukoja et al., 2018)

located_in dendrite

inferred from direct assay

(Cao et al., 2014)

part_of microtubule associated complex

inferred from mutant phenotype

(Pack-Chung et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

part_of kinesin complex

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

is_active_in microtubule

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

KINESINS

Protein Family (UniProt)

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Unc-104 subfamily. (A1ZAJ2)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065: plus-end-directed microtubule motor activity

ATP + H2O + a kinesin associated with a microtubule at position (n) = ADP + phosphate a kinesin associated with a microtubule at position (n+1, toward the plus end) (5.6.1.3)

Summaries

Gene Group (FlyBase)

KINESINS -: Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).

Protein Function (UniProtKB)

(UniProt, A1ZAJ2)

Summary (Interactive Fly)

a Kinesin-3 family member that is essential for transporting synaptic vesicle precursors

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\unc-104 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1ZAJ2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Shares 5' UTR with upstream gene.

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.44

Stop-codon suppression (UGA) postulated; FBrf0216884

Gene model reviewed during 5.56

Gene model reviewed during 6.02

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

unc-104-RB

FBtr0113087

NM_166192

7408

1670

unc-104-RC

FBtr0113088

NM_166190

7399

1670

unc-104-RD

FBtr0087079

NM_137311

7606

1739

unc-104-RE

FBtr0113089

NM_001103876

7441

1684

unc-104-RF

FBtr0304679

NM_001259444

7414

1675

unc-104-RG

FBtr0304680

NM_001259445

7432

1681

unc-104-RH

FBtr0330003

NM_001274104

7214

1673

unc-104-RI

FBtr0346959

NM_001316424

7214

1661

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

unc-104-PB

FBpp0112000

189.3

1670

6.09

FBpp0112001

189.3

1670

6.09

FBpp0086227

197.0

1739

6.04

NP_611155

AAM70884

unc-104-PE

FBpp0112002

190.8

1684

6.17

NP_001097346

ABV53825

unc-104-PF

FBpp0293221

189.6

1675

6.01

FBpp0293222

190.6

1681

6.17

FBpp0303038

189.5

1673

5.84

FBpp0312423

188.2

1661

5.92

A0A126GUN3

NP_001303353

ALI30182

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1670 aa isoforms: unc-104-PB, unc-104-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Monomer (By similarity). Interacts with Arl8 (PubMed:30174114).

(UniProt, A1ZAJ2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\unc-104 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.82

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 17

larval central nervous system

(Pack-Chung et al., 2007)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

unc-104 protein is enriched in the central nervous system of embyros from embryonic stage 11 on, particularly in synapse-rich regions.

(Pack-Chung et al., 2007)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in axon terminus

inferred from direct assay

(Shaw et al., 2019)

located_in axon

inferred from direct assay

(Cao et al., 2014)

inferred from direct assay

(Vukoja et al., 2018)

located_in dendrite

inferred from direct assay

(Cao et al., 2014)

part_of microtubule associated complex

inferred from mutant phenotype

(Pack-Chung et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\unc-104 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\unc-104

View exonic expression by tissue for Dmel\unc-104

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36876/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267002

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034155

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267002

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8566

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 25 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of unc-104

Transgenic constructs containing regulatory region of unc-104

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)Exel6064

(Ryder, 2004.4.26)

Df(2R)Exel7145

(Ryder, 2004.4.26)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

unc-104^MI07215

lethal | recessive

unc-104^bris

lethal - all die before end of larval stage (with unc-104^O3.1)

unc-104^P350

lethal - all die before end of larval stage (with unc-104^P350)

unc-104^O3.1

lethal - all die before end of pupal stage (with unc-104^O1.2)

unc-104^P350

lethal - all die before end of pupal stage (with unc-104^P350)

unc-104^O1.2

lethal - all die during embryonic stage

lethal - all die during embryonic stage (with unc-104^C674)

unc-104^P350

lethal - all die during embryonic stage (with unc-104^P350)

lethal - all die during embryonic stage (with unc-104^R767)

unc-104^P350

lethal - all die during third instar larval stage

unc-104^bris

lethal - all die during third instar larval stage (with unc-104¹⁷⁰)

unc-104^bris

lethal - all die during third instar larval stage (with unc-104^bris)

lethal - all die during third instar larval stage (with unc-104^d11204)

unc-104^bris

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

unc-104^GD13526

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Other Phenotypes

Allele

abnormal body size, with Scer\GAL4^Ilp2.PW

abnormal developmental rate, with Scer\GAL4^Ilp2.PW

unc-104^GD13526

abnormal locomotor behavior | larval stage

unc-104^bris

abnormal locomotor behavior | larval stage (with unc-104^bris)

unc-104^d11204

abnormal locomotor behavior | larval stage (with unc-104^d11204)

unc-104^bris

abnormal locomotor behavior | larval stage (with unc-104^O1.2)

unc-104^P350

abnormal locomotor behavior | larval stage (with unc-104^O3.1)

unc-104^P350

abnormal locomotor behavior | larval stage (with unc-104^P350)

abnormal neuroanatomy

abnormal neuroanatomy (with Df(2R)Alk-21)

abnormal neuroanatomy | adult stage | somatic clone

unc-104¹⁷⁰

abnormal neuroanatomy | larval stage

unc-104^bris

abnormal neuroanatomy | larval stage, with Scer\GAL4^Ilp2.PW

unc-104^GD13526

abnormal neuroanatomy | pupal stage P5, with Scer\GAL4^ppk.PU

abnormal neuroanatomy | pupal stage P5 | RU486 conditional, with Scer\GAL4^GSG2295

abnormal neuroanatomy | pupal stage P5 | somatic clone

unc-104¹⁷⁰

abnormal neuroanatomy | somatic clone | third instar larval stage

abnormal neuroanatomy | third instar larval stage

unc-104^bris

abnormal neuroanatomy | third instar larval stage (with unc-104^bris)

unc-104^d11204

abnormal neuroanatomy | third instar larval stage (with unc-104^d11204)

unc-104^bris

abnormal neurophysiology | somatic clone

abnormal neurophysiology | third instar larval stage

unc-104^bris

abnormal size | pupal stage P5, with Scer\GAL4^ppk.PU

abnormal size | pupal stage P5 | RU486 conditional, with Scer\GAL4^GSG2295

abnormal size | pupal stage P5 | somatic clone

unc-104¹⁷⁰

paralytic | embryonic stage

some die during embryonic stage

some die during embryonic stage (with unc-104^C674)

unc-104^P350

some die during embryonic stage (with unc-104^P350)

some die during embryonic stage (with unc-104^R767)

unc-104^P350

some die during pupal stage (with unc-104^O1.2)

unc-104^P350

some die during pupal stage (with unc-104^P350)

unc-104^O1.2

some die during third instar larval stage (with unc-104^O3.1)

unc-104^P350

some die during third instar larval stage (with unc-104^P350)

unc-104^O3.1

uncoordinated | larval stage (with unc-104^O1.2)

unc-104^P350

uncoordinated | larval stage (with unc-104^O3.1)

unc-104^P350

uncoordinated | larval stage (with unc-104^P350)

Phenotype manifest in

Allele

axon | larval stage | conditional, with Scer\GAL4⁴⁷⁷

unc-104^GD13526

bouton

bouton (with Df(2R)Alk-21)

bouton (with unc-104^O1.2)

unc-104^P350

bouton (with unc-104^P350)

unc-104^O1.2

bouton | third instar larval stage

unc-104^bris

dendrite & filopodium & larva

unc-104^bris

dendrite | larval stage, with Scer\GAL4²²¹

dendritic tree | larval stage, with Scer\GAL4⁴⁷⁷

unc-104^GD13526

dendritic tree | pupal stage P5, with Scer\GAL4^ppk.PU

dendritic tree | pupal stage P5 | RU486 conditional, with Scer\GAL4^GSG2295

dendritic tree | pupal stage P5 | somatic clone

unc-104¹⁷⁰

dense core granule & axon (with unc-104^O1.2)

unc-104^P350

dense core granule & axon (with unc-104^O3.1)

unc-104^P350

dense core granule & axon (with unc-104^P350)

dorsal multidendritic neuron ddaA & filopodium

unc-104^bris

embryonic/larval neuromuscular junction (with unc-104^O1.2)

unc-104^P350

embryonic/larval neuromuscular junction (with unc-104^P350)

unc-104^O1.2

embryonic/larval neuromuscular junction | third instar larval stage

unc-104^bris

filopodium | increased number | third instar larval stage

unc-104^bris

larval dorsal multidendritic neuron ddaA | third instar larval stage

unc-104^bris

larval dorsal multidendritic neuron ddaC | larval stage, with Scer\GAL4⁴⁷⁷

unc-104^GD13526

larval dorsal multidendritic neuron ddaC | larval stage | conditional, with Scer\GAL4⁴⁷⁷

unc-104^GD13526

larval dorsal multidendritic neuron ddaC | pupal stage P5, with Scer\GAL4^ppk.PU

larval dorsal multidendritic neuron ddaC | pupal stage P5 | RU486 conditional, with Scer\GAL4^GSG2295

larval dorsal multidendritic neuron ddaC | pupal stage P5 | somatic clone

unc-104¹⁷⁰

larval dorsal multidendritic neuron ddaE | larval stage, with Scer\GAL4²²¹

larval insulin secreting cell of pars intercerebralis, with Scer\GAL4^Ilp2.PW

unc-104^GD13526

larval intersegmental nerve branch ISNb of A1-7

larval intersegmental nerve branch ISNb of A1-7 (with Df(2R)Alk-21)

microtubule | larval stage, with Scer\GAL4²²¹

microtubule | larval stage | conditional, with Scer\GAL4⁴⁷⁷

unc-104^GD13526

neuromuscular junction | third instar larval stage

unc-104^bris

photoreceptor cell R7 | somatic clone

unc-104¹⁷⁰

postsynaptic density | third instar larval stage

unc-104^bris

subsynaptic reticulum | third instar larval stage

unc-104^bris

synapse

synapse (with Df(2R)Alk-21)

synaptic vesicle (with unc-104^O1.2)

unc-104^P350

synaptic vesicle (with unc-104^O3.1)

unc-104^P350

synaptic vesicle (with unc-104^P350)

terminal bouton

unc-104¹⁷⁰

terminal bouton (with Df(2R)Alk-21)

unc-104¹⁷⁰

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (41)

Hsap\KIF1A

13 of 14

Yes

Hsap\KIF1B

11 of 14

Yes

Hsap\KIF1C

7 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\KIFC1

1 of 14

Hsap\KIFC3

1 of 14

Hsap\SKAP1

1 of 14

Yes

Hsap\SKAP2

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (24)

Rnor\Kif1a

13 of 14

Yes

Rnor\Kif1b

10 of 14

Yes

Rnor\Kif1c

7 of 14

Yes

Rnor\Kif28p

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Skap2

1 of 14

Yes

Mus musculus (laboratory mouse) (24)

Mmus\Kif1a

12 of 14

Yes

Mmus\Kif1b

11 of 14

Yes

Mmus\Kif1c

7 of 14

Yes

Mmus\Kif14

4 of 14

Mmus\Kif28

4 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Skap2

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (29)

Xtro\kif1a

8 of 13

Yes

Xtro\kif1b

7 of 13

Yes

Xtro\kif1c

4 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\kifc2

1 of 13

Xtro\klhdc8b

1 of 13

Yes

Xtro\LOC100490157

1 of 13

Xtro\LOC100490836

1 of 13

Yes

Xtro\LOC100491511

1 of 13

Yes

Xtro\LOC108648793

1 of 13

Xtro\skap1

1 of 13

Xtro\skap2

1 of 13

Yes

Danio rerio (Zebrafish) (28)

Drer\kif1aa

13 of 14

Yes

Drer\kif1ab

11 of 14

Yes

Drer\kif1b

11 of 14

Yes

Drer\kif1c

5 of 14

Yes

Drer\si:ch73-375g18.1

5 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\skap2

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\unc-104

13 of 14

Yes

Cele\klp-6

4 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (17)

Agam\KIF1A_ANOGA

11 of 12

Yes

3 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (51)

Atha\AT3G44050

2 of 13

Yes

Atha\KINESIN-12B

2 of 13

Yes

Atha\PAKRP1

2 of 13

Yes

Atha\POK1

2 of 13

Yes

Atha\POK2

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

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1 of 13

1 of 13

1 of 13

1 of 13

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1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\KIP1

1 of 13

Yes

Scer\KIP3

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\klp5

1 of 12

Yes

Spom\klp6

1 of 12

Yes

Spom\klp8

1 of 12

Yes

Spom\tea2

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:unc-104. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (26)

Khc-73

6 of 13

Klp98A

6 of 13

neb

6 of 13

Khc

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

pav

3 of 13

sub

3 of 13

cmet

2 of 13

cos

2 of 13

Klp3A

2 of 13

Klp54D

2 of 13

Klp61F

2 of 13

ncd

2 of 13

nod

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 6 )

Allele

Disease

Evidence

References

unc-104^P350

model of nervous system disease

CEA

(Li et al., 2017)

CEA with unc-104^bris

(Li et al., 2017)

unc-104^O3.1

model of nervous system disease

CEA with unc-104^bris

(Li et al., 2017)

unc-104¹⁷⁰

model of nervous system disease

CEA

(Li et al., 2017)

unc-104⁵²

model of nervous system disease

CEA

(Li et al., 2017)

unc-104^bris

DOES NOT model hereditary spastic paraplegia

CEA

(Kern et al., 2013)

model of nervous system disease

CEA

(Zhang et al., 2016)

CEA with unc-104^P350

(Li et al., 2017)

CEA with unc-104^bris

(Li et al., 2017)

CEA with unc-104^O3.1

(Li et al., 2017)

unc-104^GD13526

model of nervous system disease

CEA

(Li et al., 2017)

Potential Models Based on Orthology ( 6 )

Human Ortholog

Disease

Evidence

References

KIF1A; kinesin family member 1A

model of hereditary spastic paraplegia 30B

IEA

(FlyBase, 2019-)

model of NESCAV syndrome

IEA

(FlyBase, 2019-)

model of hereditary sensory neuropathy type 2C

IEA

(FlyBase, 2019-)

model of hereditary spastic paraplegia 30A

IEA

(FlyBase, 2019-)

KIF1B; kinesin family member 1B

model of Charcot-Marie-Tooth disease type 2A1

IEA

(FlyBase, 2019-)

KIF1C; kinesin family member 1C

model of spastic ataxia 2

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 7 )

Allele

Disease

Interaction

References

unc-104^P350

ameliorates Parkinson's disease 8

modeled by Lrrk¹

(Inoshita et al., 2022)

model of nervous system disease

is ameliorated by wnd³

(Li et al., 2017)

unc-104^{UAS.RVB.GFP(S65T)}

exacerbates Parkinson's disease 8

modeled by Lrrk¹

(Inoshita et al., 2022)

unc-104¹⁷⁰

model of nervous system disease

is ameliorated by wnd³

(Li et al., 2017)

exacerbates tauopathy

modeled by shot³, tau^MR22

(Voelzmann et al., 2016)

unc-104⁵²

model of nervous system disease

is ameliorated by wnd³

(Li et al., 2017)

unc-104^UAS.cPCa

ameliorates tauopathy

modeled by shot³, tau^MR22

(Voelzmann et al., 2016)

unc-104^bris

model of nervous system disease

is ameliorated by Rab3^UAS.cGa

(Zhang et al., 2016)

is ameliorated by brp^UAS.cWa

(Zhang et al., 2016)

is exacerbated by brp^RNAi.C.UAS

(Zhang et al., 2016)

unc-104^GD13526

model of nervous system disease

is ameliorated by wnd³

(Li et al., 2017)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KIF1A; kinesin family member 1A

13 of 14

601255

KIF1B; kinesin family member 1B

11 of 14

605995

Charcot-Marie-Tooth disease type 2A1

KIF1C; kinesin family member 1C

7 of 14

603060

SPASTIC ATAXIA 2, AUTOSOMAL RECESSIVE; SPAX2

spastic ataxia 2

KIF14; kinesin family member 14

4 of 14

611279

primary autosomal recessive microcephaly 20

KIF13A; kinesin family member 13A

3 of 14

605433

KIF13B; kinesin family member 13B

3 of 14

607350

KIF16B; kinesin family member 16B

3 of 14

618171

3 of 14

614642

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

unc-104 - AP-2α

anti tag coimmunoprecipitation, anti tag western blot

(Zong et al., 2018)

unc-104 - Rab2

proximity-dependent biotin identification, western blot

(Lund et al., 2021)

unc-104 - Rab39

pull down, peptide massfingerprinting, western blot

(Gillingham et al., 2014, Gillingham et al., 2014)

unc-104 - prd1

anti tag coimmunoprecipitation, anti tag western blot

(Zong et al., 2018)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

unc-104

enhanceable

AP-2α

(Zong et al., 2018)

unc-104

suppressible

bsk

(Chen et al., 2012)

unc-104

suppressible

Nrg

(Zong et al., 2018)

unc-104

suppressible

γTub23C

(Chen et al., 2012)

unc-104

enhanceable

γTub23C

(Chen et al., 2012)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

brm

suppressible

unc-104

(Tegeder et al., 2019)

prd1

enhanceable

unc-104

(Zong et al., 2018)

External Data

Subunit Structure (UniProtKB)

Monomer (By similarity). Interacts with Arl8 (PubMed:30174114).

(UniProt, A1ZAJ2 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0267002

MIST (protein-protein) - An integrated Molecular Interaction Database

36876

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene

(Medina et al., 2006)

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-80

Cytogenetic map

53D6-53D8

Sequence location

2R:16,751,503..16,772,671 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

53D6-53D8

Limits computationally determined from genome sequence between P{PZ}Sema-2a⁰³⁰²¹&P{PZ}Cdk4⁰⁵⁴²⁸ and P{EP}BEST:SD02913^EP2148&P{lacW}Dek^k09907;

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

53D-53D

(Grossberger, 2000.3.23)

Experimentally Determined Recombination Data

Location

2-80

(FlyBase, 2016)

2-84.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (23)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

24786

P{UAS-unc-104.GFP.RVB}1, w^*

24787

w^*; P{UAS-unc-104.GFP.RVB}2

24788

w^*; P{UAS-unc-104.GFP.RVB}3

28951

y¹ v¹; P{TRiP.HM05162}attP2

24316

w¹¹¹⁸; Mi{ET1}unc-104^MB05021

29153

w¹¹¹⁸; Mi{ET1}unc-104^MB10763

19346

w¹¹¹⁸; P{XP}unc-104^d11204/CyO

53296

y¹ v¹; P{TRiP.HMC03512}attP40

58191

y¹ v¹; P{TRiP.HMJ21840}attP40

24630

y¹ w^*; unc-104^P350/CyO

VDRC

v23465

w¹¹¹⁸; P{GD13526}v23465

v47171

w¹¹¹⁸; P{GD16406}v47171/TM3

More stocks available...

Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (37)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0267002

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Pack-Chung et al., 2007)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: unc-104 bris

(Kern et al., 2013)

Additional comments

Source for identity of unc-104 CG8566 was sequence comparison ( date:000607 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: unc-104 CG8566

(FlyBase, 1996-)

Nomenclature comments

Etymology

Named "unc-104" after the C. elegans ortholog.

(FlyBase, 2009-)

Synonyms and Secondary IDs (31)

Reported As

Symbol Synonym

CG8566

(National Institute of Genetics Fly Stocks, 2022-, Yamaguchi and Takashima, 2018, Ni et al., 2010.12.1, Perkins et al., 2009, Berger et al., 2008, Pack-Chung et al., 2007, Wagstaff and Begun, 2005, Philipps et al., 2004, Miki et al., 2001, Goldstein and Gunawardena, 2000)

DUNC104

(Miki et al., 2005)

DUnc-104

(Hirokawa, 2012.11.20)

DmCG8566

(Endow, 2001.4.14)

DmDUnc104

(Hirokawa, 2012)

DmKlp53D

(Dagenbach and Endow, 2004, Endow, 2001.4.14, Kim and Endow, 2000)

DmUNC-104

(Niwa et al., 2024)

Dunc104

(Grossberger, 2000.3.23)

Imac

(Rui, 2024, Siebert et al., 2015, Sugie et al., 2015)

KIF 1B

(Miki et al., 2001)

KIF1A

(Yamaguchi et al., 2021, Kern et al., 2013)

KIF1A/1B

(Hirokawa, 2012.11.20)

Kif1a

(Herrera and Bach, 2021, Rodriguez-Fernandez et al., 2020, Hu and Jasper, 2019)

Klp53D

(Pack-Chung et al., 2007, Kural et al., 2005, Goshima and Vale, 2003, Vale, 2003)

Unc-104

(Tsakiri et al., 2025, Liguori et al., 2021, Li et al., 2017, Lim et al., 2017, Nässel and Vanden Broeck, 2016, Gillingham et al., 2014, Zhang et al., 2013, Barkus et al., 2008, Barkus et al., 2008, Liebl and Featherstone, 2008)

Unc104

(Jiang et al., 2019, Phan et al., 2019, Winding et al., 2016, Ally et al., 2009)

bris

(Kern et al., 2013, Medina et al., 2006)

imac

(Shaw et al., 2019, Zong et al., 2018, Wang et al., 2015, Holbrook et al., 2012, Rui et al., 2010, Johnson et al., 2009, Kurshan et al., 2009, Haddad et al., 2008, Toda et al., 2008, Pack-Chung et al., 2007, Pack-Chung et al., 2004)

kif1a

(Gan et al., 2021)

unc104

(Akasaka et al., 2020, Reid et al., 2012)

Name Synonyms

D Uncoordinated Protein 104

(Hirokawa, 2012.11.20)

Kinesin-3

(Kern et al., 2013)

Kinesin4B

(Kollmar, 2015.9.15)

bristly

(Kern et al., 2013, Medina et al., 2006)

immaculate connections

(Johnson et al., 2009, Pack-Chung et al., 2007)

kinesin 3

(Johnson et al., 2009)

kinesin-3

(Lim et al., 2017)

unc-104 ortholog

uncoordinated 104

(Yamaguchi and Takashima, 2018)

uncoordinated-104

Secondary FlyBase IDs

FBgn0034155
FBgn0086297

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 67 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36876

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1ZAJ2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A1ZAJ2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1ZAJ2

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36876/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267002

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267002

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8566

MARRVEL_MODEL - MARRVEL (model organism gene)

36876

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0267002

DroID - A comprehensive database of gene and protein interactions.

FBgn0267002

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034155

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0267002

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8566

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0267002

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006253

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

MIST (protein-protein) - An integrated Molecular Interaction Database

36876

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (140)

The following external sites may use different assemblies or annotations than FlyBase.

-0.82

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