FlyBase Allele Report: Dmel\unc-104[GD16406]

General Information

Symbol

Dmel\unc-104^GD16406

Species

D. melanogaster

Name

FlyBase ID

FBal0210832

Feature type

allele

Associated gene

Dmel\unc-104

Associated Insertion(s)

Carried in Construct

P{GD16406}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD16406}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD16406

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | pupal stage P5, with Scer\GAL4^ppk.PU

(Zong et al., 2018)

abnormal size | pupal stage P5, with Scer\GAL4^ppk.PU

(Zong et al., 2018)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of unc-104^GD16406 under the control of Scer\GAL4^ppk.PU (and Dicer-2, for efficient RNAi) induces severe and fully penetrant ddaC neuron dendrite pruning defects at 16h after puparium formation, as compared to controls; these neurons do not present any obvious changes in lysosomes (i.e. Lysotracker staining), secretory vesicles (Sec15) or Golgi apparatus (i.e. GalT), as compared to controls.

(Zong et al., 2018)

Larval ddaE neurons expressing unc-104^GD16406 under the control of Scer\GAL4²²¹ (and Dicer-2, for a more efficient RNAi) show a significant increase in growing microtubules within dendrites, as compared to neurons in controls.

(Chen et al., 2012)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16406

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(3R)Exel7310, unc-104^GD16406 has abnormal neuroanatomy | pupal stage P5 phenotype, enhanceable by AP-2alpha[+]/AP-2α³

(Zong et al., 2018)

Df(3R)Exel7310, unc-104^GD16406 has abnormal size | pupal stage P5 phenotype, enhanceable by AP-2alpha[+]/AP-2α³

(Zong et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PU, unc-104^GD16406 has abnormal neuroanatomy | pupal stage P5 phenotype, suppressible | partially by Nrg^GL00656, Scer\GAL4^ppk.PU

(Zong et al., 2018)

Scer\GAL4^ppk.PU, unc-104^GD16406 has abnormal size | pupal stage P5 phenotype, suppressible | partially by Nrg^GL00656, Scer\GAL4^ppk.PU

(Zong et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(3R)Exel7310, unc-104^GD16406 has dendritic tree | pupal stage P5 phenotype, enhanceable by AP-2alpha[+]/AP-2α³

(Zong et al., 2018)

Df(3R)Exel7310, unc-104^GD16406 has larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype, enhanceable by AP-2alpha[+]/AP-2α³

(Zong et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PU, unc-104^GD16406 has dendritic tree | pupal stage P5 phenotype, suppressible | partially by Nrg^GL00656, Scer\GAL4^ppk.PU

(Zong et al., 2018)

Scer\GAL4^ppk.PU, unc-104^GD16406 has larval dorsal multidendritic neuron ddaC | pupal stage P5 phenotype, suppressible | partially by Nrg^GL00656, Scer\GAL4^ppk.PU

(Zong et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The ddaC neuron pruning defects induced by the expression of unc-104^GD16406 under the control of Scer\GAL4^ppk.PU (and Dicer-2, for efficient RNAi) are significantly enhanced by heterozygosity for AP-2α³, as the persisting dendrites become significantly longer.

(Zong et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v47171

w¹¹¹⁸; P{GD16406}v47171/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16406

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

unc-104^GD16406

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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