FlyBase Gene Report: Dmel\Klp68D

General Information

Symbol

Dmel\Klp68D

Species

D. melanogaster

Name

Kinesin-like protein at 68D

Annotation Symbol

CG7293

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004381

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Kinesin-like protein at 68D (Klp68D) encodes the kinesin-2β motor subunit of the heterotrimeric kinesin-2. It associates with the products of Klp64D and Kap3. It plays an essential role in the sensory cilia assembly, microtubule organization in dendrites, and axonal transport. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

DmKlp68D, kinesin II, KLP5, kinesin-2

Key Links

Genomic Location

Cytogenetic map

68D6-68E1

Sequence location

3L:11,795,614..11,798,875 [+]

Recombination map

3-36

RefSeq locus

NT_037436 REGION:11795614..11798875

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables cytoskeletal motor activity

inferred from direct assay

(Pesavento et al., 1994)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

enables cytoskeletal motor activity

inferred from sequence or structural similarity with MGI:MGI:107689

(Pesavento et al., 1994)

enables microtubule binding

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001752

(InterPro Project Members, 2004-)

enables microtubule motor activity

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821, InterPro:IPR027640

(InterPro Project Members, 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in anterograde axonal transport

inferred from expression pattern

(Pesavento et al., 1994)

inferred from mutant phenotype

(Baqri et al., 2006)

involved_in establishment or maintenance of microtubule cytoskeleton polarity

inferred from mutant phenotype

(Mattie et al., 2010)

involved_in microtubule-based movement

inferred from mutant phenotype

(Pesavento et al., 1994)

involved_in sensory perception of smell

inferred from mutant phenotype

(Jana et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in microtubule-based movement

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821, InterPro:IPR027640

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of microtubule associated complex

inferred from direct assay

(Pesavento et al., 1994)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

part_of kinesin complex

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

is_active_in microtubule

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

KINESINS

Protein Family (UniProt)

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily. (P46867)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Snapshot

Gene Group (FlyBase)

KINESINS -: Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).

Protein Function (UniProtKB)

Plus-end directed microtubule motor that may be used for anterograde axonal transport and could conceivably move cargos in fly neurons different than those moved by kinesin heavy chain or other plus-end directed motors.

(UniProt, P46867)

Summary (Interactive Fly)

cytoskeletonal motor protein - Klp64D and Klp68D are kinesin-2α kinesin-2β motor subunits of the heterotrimeric kinesin-2 - Klp64D associates with Klp68D and Kap3 - Klp64D and Klp68D play an essential role in sensory cilia assembly and axonal transport - microtubule organization in dendrites - hearing

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Klp68D for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P46867)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Klp68D-RA

FBtr0076030

NM_079305

2998

784

Klp68D-RB

FBtr0332129

NM_001274797

2971

784

Additional Transcript Data and Comments

Reported size (kB)

3.1 (northern blot)

(Stewart et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Klp68D-PA

FBpp0075762

88.2

784

8.17

FBpp0304439

88.2

784

8.17

P46867

NP_001261726

AGB94420

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

784 aa isoforms: Klp68D-PA, Klp68D-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Klp68D using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Klp68D_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.88

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

indirect flight muscle cell

(Katti et al., 2017)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

embryonic central brain neuron

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

sensory nervous system primordium

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

ventral sensory complex primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Stewart et al., 1991)

adult stage

head

(Stewart et al., 1991)

adult stage | female

ovary

(Stewart et al., 1991)

adult stage | male

testis

(Stewart et al., 1991)

Additional Descriptive Data

Klp68D is expressed at all stages and in all tissues tested in adults (heads, male bodies, testis, female bodies, ovary). It is most abundant in heads and ovaries in adults.

(Stewart et al., 1991)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female & oogenesis

nurse cell

(Sarpal and Ray, 2002)

oogenesis & adult stage | female

female germline cell

(Sarpal and Ray, 2002)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of microtubule associated complex

inferred from direct assay

(Pesavento et al., 1994)

Expression Deduced from Reporters

Reporter: P{Klp68D-GAL4.S}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

Johnston organ neuron

(Senthilan et al., 2012)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Klp68D in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Klp68D

View exonic expression by tissue for Dmel\Klp68D

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004381

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39332/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004381

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004381

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004381

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7293

Flygut - An atlas of the Drosophila adult midgut

FBgn0004381

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Klp68D

Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Klp68D

Transgenic constructs containing regulatory region of Klp68D

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Klp68D^GD12196

lethal | recessive

Klp68D^Bex2

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

Klp68D^GD12196

viable

Klp68D^EY00199

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Klp68D^GD12196

viable, with Scer\GAL4^tin.CΔ4

Klp68D^GD12196

Sterility

Allele

fertile

Klp68D^EY00199

Other Phenotypes

Allele

abnormal auditory perception | adult stage

Klp68D^EY00199

abnormal neuroanatomy

Klp68D^KG03849

abnormal neurophysiology

visible, with Scer\GAL4^pnr-MD237

Klp68D^GD12196

Phenotype manifest in

Allele

basiconic sensillum of antennal segment 3

Klp68D^KG03849

cilium

Klp68D^KG03849

mesonotum, with Scer\GAL4^pnr-MD237

Klp68D^GD12196

trichogen cell, with Scer\GAL4^pnr-MD237

Klp68D^GD12196

trichoid sensillum of antennal segment 3

Klp68D^KG03849

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (45)

Hsap\KIF3B

13 of 14

Yes

Hsap\KIF3C

12 of 14

Yes

Hsap\KIF3A

4 of 14

Hsap\KIF6

2 of 14

Yes

Hsap\KIF12

2 of 14

Hsap\KIF15

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (37)

Rnor\Kif3b

13 of 14

Yes

Rnor\Kif3c

11 of 14

Yes

Rnor\Kif3a

4 of 14

Rnor\Kif6

2 of 14

Yes

Rnor\Kif9

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (41)

Mmus\Kif3b

12 of 14

Yes

Mmus\Kif3c

11 of 14

Yes

Mmus\Kif3a

4 of 14

Mmus\Kif6

2 of 14

Yes

Mmus\Kif9

2 of 14

Yes

Mmus\Kif12

2 of 14

Mmus\Kif15

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (25)

Xtro\kif3b

8 of 13

Yes

Xtro\kif3c

5 of 13

Yes

Xtro\bbs2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (40)

Drer\kif3b

12 of 14

Yes

Drer\kif3c

12 of 14

Yes

Drer\kif3cb

11 of 14

Yes

Drer\kif3a

4 of 14

Drer\kif17

3 of 14

Drer\kif6

2 of 14

Yes

Drer\kif9

2 of 14

Yes

Drer\kif15

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-257p13.3

1 of 14

Drer\si:dkey-26i13.8

1 of 14

Drer\si:dkey-96l17.6

1 of 14

Drer\zgc:103670

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (19)

Cele\klp-11

11 of 14

Yes

4 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (22)

Agam\AgaP_AGAP010396

11 of 12

Yes

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (57)

Atha\AT1G09170

2 of 13

Yes

Atha\AT1G18410

2 of 13

Yes

Atha\AT1G72250

2 of 13

Yes

Atha\AT1G73860

2 of 13

Yes

Atha\AT2G22610

2 of 13

Yes

Atha\AT2G47500

2 of 13

Yes

Atha\AT3G44050

2 of 13

Yes

Atha\AT5G27550

2 of 13

Yes

Atha\AT5G41310

2 of 13

Yes

Atha\ATK4

2 of 13

Yes

Atha\KINESIN-12B

2 of 13

Yes

Atha\KP1

2 of 13

Yes

Atha\PAKRP1

2 of 13

Yes

Atha\POK2

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\CIN8

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\klp3

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Klp68D. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (28)

Klp64D

8 of 13

Kif3C

7 of 13

Khc

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

neb

3 of 13

nod

3 of 13

pav

3 of 13

sub

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

cos

2 of 13

Klp3A

2 of 13

ncd

2 of 13

CG5004

1 of 13

Gγ1

1 of 13

Psf1

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Klp68D^GD12196

model of chromosome 16p11.2 deletion syndrome, 593-kb

CEA

(Iyer et al., 2018)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

KIF3B; kinesin family member 3B

model of retinitis pigmentosa 89

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KIF3B; kinesin family member 3B

13 of 14

603754

RETINITIS PIGMENTOSA 89; RP89

retinitis pigmentosa 89

KIF3C; kinesin family member 3C

12 of 14

602845

KIF3A; kinesin family member 3A

4 of 14

604683

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Klp68D - Ace

anti bait coimmunoprecipitation, western blot

(Baqri et al., 2006)

Klp68D - ChAT

anti tag coimmunoprecipitation, western blot, fluorescent resonance energy transfer, fluorescence microscopy, anti bait coimmunoprecipitation

(Dey and Ray, 2018, Sadananda et al., 2012, Baqri et al., 2006)

Klp68D - Kap3

anti bait coimmunoprecipitation, western blot, affinity chromatography technology, pull down, cosedimentation, anti tag western blot, molecular weight estimation by staining

(Ahmed et al., 2020, Ahmed et al., 2019, Doodhi et al., 2009, Baqri et al., 2006)

Klp68D - Klp64D

anti bait coimmunoprecipitation, western blot, affinity chromatography technology, molecular weight estimation by staining, fluorescent resonance energy transfer, molecular sieving, fluorescence microscopy, cosedimentation, pull down, circular dichroism, anti tag coimmunoprecipitation, anti tag western blot

(Ahmed et al., 2020, Ahmed et al., 2019, Girotra et al., 2017, Doodhi et al., 2012, Sadananda et al., 2012, Doodhi et al., 2009)

Klp68D - Or47b

pull down, western blot

(Jana et al., 2021)

Klp68D - Orco

anti tag coimmunoprecipitation, western blot, pull down, anti tag western blot

(Jana et al., 2021)

Klp68D - αTub84B

far western blotting, anti tag western blot, fluorescent resonance energy transfer, fluorescence microscopy

(Girotra et al., 2017)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64693

DroID - A comprehensive database of gene and protein interactions.

FBgn0004381

MIST (protein-protein) - An integrated Molecular Interaction Database

39332

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-36

Cytogenetic map

68D6-68E1

Sequence location

3L:11,795,614..11,798,875 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

68D6-68E1

Limits computationally determined from genome sequence between P{PZ}l(3)01239⁰¹²³⁹&P{lacW}l(3)01239^j9B4 and P{PZ}CycA⁰²⁴⁶¹&P{PZ}CycA⁰³⁹⁴⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-36

(FlyBase, 2016)

3-30.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

29410

y¹ v¹; P{TRiP.JF03346}attP2

20090

y¹ w^67c23; P{EPgy2}Klp68D^EY00199

13511

y¹; P{SUPor-P}Klp68D^KG03849 ry⁵⁰⁶

56237

y¹ w^*; Mi{MIC}Klp68D^MI10747/TM3, Sb¹ Ser¹

603133

y¹ w^*; TI{KozakGAL4}Klp68D^{CR71223-KO-kG4}/TM3, Sb¹ Ser¹

7293R-1

7293R-2

v101058

v27944

w¹¹¹⁸; P{GD12196}v27944

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (39)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH23075

Other clones

DPiM_GH23075

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004381

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004381

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Doodhi et al., 2012, Jana et al., 2011)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Molecular and biochemical properties of Klp68D suggest it may be used for anterograde axon transport and could move cargoes in neurons different to those moved by kinesin heavy chain or other plus-end directed motors.

(Pesavento et al., 1994)

Klp68D has been cloned, partially sequenced and its expression pattern analysed.

(Stewart et al., 1991)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Klp68D CG7293

(Zhang, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

CG7293

(Neuert et al., 2017, Chen et al., 2015, Hosono et al., 2015, Keleman et al., 2009.8.5, Perkins et al., 2009, Avidor-Reiss et al., 2004, Goldstein and Gunawardena, 2000)

DmKlp68D

(Hirokawa, 2012, Dagenbach and Endow, 2004, Kim and Endow, 2000, Moore and Endow, 1996)

KIF 3B

(Miki et al., 2001)

KIF3B

(Hirokawa, 2012.11.20)

KLP-5

(Pesavento and Goldstein, 1991)

KLP5

(Stewart et al., 1991)

KLP68D

(Ahmed et al., 2020, Ahmed et al., 2019, Dey and Ray, 2018, Girotra et al., 2017, Sadananda et al., 2012, Doodhi et al., 2009, Vendra et al., 2007, Miki et al., 2005, Lawrence et al., 2002, Sarpal and Ray, 2002, Ray et al., 1999, Hirokawa et al., 1998, Perez et al., 1998, Pesavento et al., 1994)

KLP68Ddm

(Still et al., 2004)

KLP_64D/68D

(Hirokawa, 1997)

Klp5

klp68d

(Goldstein, 1994.10.15)

Name Synonyms

Kinesin-2

(Vendra et al., 2007)

Kinesin-like protein at 68D

(Hirokawa, 2012.11.20)

Kinesin1

(Kollmar, 2015.9.15)

kinesin II

(Welte, 2004, Witman, 2003, Mazumdar and Mazumdar, 2002, Lane and Allan, 1998)

kinesin-2

(Girotra et al., 2017, Sadananda et al., 2012)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 49 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39332

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P46867

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P46867

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PF68

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P46867

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004381

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39332/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004381

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004381

FlyMine - An integrated database for Drosophila genomics

FBgn0004381

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7293

MARRVEL_MODEL - MARRVEL (model organism gene)

39332

Linkouts

BioGRID - A database of protein and genetic interactions.

64693

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004381

DroID - A comprehensive database of gene and protein interactions.

FBgn0004381

DRSC - Results frm RNAi screens

FBgn0004381

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Klp68D_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004381

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004381

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7293

Flygut - An atlas of the Drosophila adult midgut

FBgn0004381

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0004381

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004397

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

MIST (protein-protein) - An integrated Molecular Interaction Database

39332

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (111)

The following external sites may use different assemblies or annotations than FlyBase.

-0.88

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