FlyBase Gene Report: Dmel\cmet

General Information

Symbol

Dmel\cmet

Species

D. melanogaster

Name

CENP-meta

Annotation Symbol

CG6392

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040232

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

CENP-meta (cmet) encodes a kinetochore kinesin that contributes to metaphase chromosome alignment. [Date last reviewed: 2019-06-27] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Cenp-E, DmCmeta, l(2)04431

Key Links

Genomic Location

Cytogenetic map

32E2-32E2

Sequence location

2L:11,261,026..11,269,002 [-]

Recombination map

2-44

RefSeq locus

NT_033779 REGION:11261026..11269002

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821

(InterPro Project Members, 2004-)

enables microtubule binding

inferred from biological aspect of ancestor with PANTHER:PTN001947122

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001752

(InterPro Project Members, 2004-)

enables microtubule motor activity

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821, InterPro:IPR027640

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:Q02224

(Gene Ontology Curators, 2002-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in mitotic metaphase chromosome alignment

inferred from mutant phenotype

(Yucel et al., 2000)

involved_in regulation of cell cycle

inferred from mutant phenotype

(Clemente-Ruiz et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in microtubule-based movement

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821, InterPro:IPR027640

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN001947122

(GO Reference Genome Project, 2011-)

involved_in mitotic cell cycle

inferred from biological aspect of ancestor with PANTHER:PTN001947122

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in kinetochore

inferred from direct assay

(Yucel et al., 2000)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in microtubule

inferred from biological aspect of ancestor with PANTHER:PTN001947122

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

KINESINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

CENP-meta (cmet) encodes a kinetochore kinesin that contributes to metaphase chromosome alignment. [Date last reviewed: 2019-06-27]

Gene Group (FlyBase)

KINESINS -: Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\cmet for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VKI0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

cmet-RC

FBtr0332202

NM_080254

7008

2189

cmet-RD

FBtr0332203

NM_001273451

6843

2134

cmet-RE

FBtr0332204

NM_001273452

6999

2186

cmet-RF

FBtr0332205

NM_001273453

6837

2132

Additional Transcript Data and Comments

Reported size (kB)

7.524 (longest cDNA)

(Yucel et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

cmet-PC

FBpp0304511

251.5

2189

4.84

FBpp0304512

245.0

2134

4.87

FBpp0304513

251.2

2186

4.83

FBpp0304514

244.8

2132

4.87

M9PFL9

NP_001260382

AGB92917

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

2244 (aa); 250, 180 (kD observed); 257 (kD predicted)

(Yucel et al., 2000)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cmet using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

cmet_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.37

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

wing pouch | restricted

(Molnar et al., 2012)

wing hinge primordium | restricted

(Molnar et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

cmet associates with centromeric regions of chromosomes, probably kinetochores.

(Yucel et al., 2000)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in kinetochore

inferred from direct assay

(Yucel et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\cmet in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\cmet

View exonic expression by tissue for Dmel\cmet

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53561/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040232

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040232

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040232

Flygut - An atlas of the Drosophila adult midgut

FBgn0040232

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of cmet

Transgenic constructs containing regulatory region of cmet

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene disrupted in

Df(2L)ab-c1

(Sawamura et al., 2010)

Df(2L)BSC236

(Christensen and Cook, 2006.12.5)

Df(2L)BSC241

(Christensen and Cook, 2006.12.5)

Df(2L)BSC242

(Christensen and Cook, 2008.4.15)

Df(2L)BSC343

(Christensen and Cook, 2007.10.29)

Df(2L)Exel6028

(Ryder, 2004.4.26)

Df(2L)Prl

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2L)salm^FRT

(Spletter et al., 2015)

Gene partially disrupted in

Df(2L)Cenp-E141

(Radford et al., 2015)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

cmet^GD11911

lethal (with Df(2L)Cenp-E141)

cmet^Δ

lethal | embryonic stage | maternal effect

cmet⁰⁴⁴³¹

lethal | recessive

cmet⁰⁴⁴³¹

lethal - all die before end of larval stage | recessive

cmet⁰⁴⁴³¹

viable

cmet^EY00718

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

cmet^GD11911

viable, with Scer\GAL4^Mef2.PR

cmet^GD11911

viable, with Scer\GAL4^pnr-MD237

cmet^GD11911

viable, with Scer\GAL4^tin.CΔ4

cmet^GD11911

Sterility

Allele

fertile

cmet^EY00718

Other Phenotypes

Allele

abnormal mitotic cell cycle

cmet^Δ

abnormal mitotic cell cycle | recessive

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

cmet^GD11911

abnormal size | adult stage

abnormal size | adult stage | conditional

abnormal size | adult stage | heat sensitive

decreased cell number | germline clone | oogenesis

cmet^Δ

some die during larval stage | recessive

cmet⁰⁴⁴³¹

visible, with Scer\GAL4^pnr-MD237

cmet^GD11911

visible | adult stage

visible | adult stage | conditional

visible | adult stage | heat sensitive

Phenotype manifest in

Allele

centrosome | germline clone

cmet^1x1

chromosome, with Scer\GAL4^{VP16.mat.αTub67C}

cmet^GL00404

condensed nuclear chromosome

cmet^Δ

embryo | germline clone | maternal effect

cmet^Δ

embryonic/first instar larval cuticle | maternal effect

cmet⁰⁴⁴³¹

eye

eye | conditional

eye | heat sensitive

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

cmet^GD11911

karyosome, with Scer\GAL4^{VP16.mat.αTub67C}

cmet^GL00404

larval brain | larval stage

cmet^1x1

mitosis & nuclear chromosome

mitosis & nuclear chromosome | germ-line clone

mitotic anaphase | germline clone

mitotic cell cycle

mitotic metaphase | germline clone

spermatocyte

cmet^Δ

trichogen cell, with Scer\GAL4^pnr-MD237

cmet^GD11911

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (33)

Hsap\CENPE

10 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Hsap\CCDC150

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (20)

Rnor\Cenpe

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (25)

Mmus\Cenpe

10 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (18)

Xtro\cenpe

5 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (22)

Drer\cenpe

9 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\klp-18

2 of 14

Yes

Cele\unc-116

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (16)

Agam\AgaP_AGAP006472

8 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (46)

Atha\ZCF125

7 of 13

Yes

Atha\AT3G10180

6 of 13

Yes

Atha\AT1G21730

3 of 13

Yes

Atha\AT2G21300

3 of 13

Yes

Atha\AT2G21380

3 of 13

Yes

Atha\AT3G12020

3 of 13

Yes

Atha\AT3G51150

3 of 13

Yes

Atha\AT4G24170

3 of 13

Yes

Atha\AT4G38950

3 of 13

Yes

Atha\AT4G39050

3 of 13

Yes

Atha\AT5G06670

3 of 13

Yes

Atha\AT5G66310

3 of 13

Yes

Atha\HIK

3 of 13

Yes

Atha\TES

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT1G55550

1 of 13

Atha\AT1G67170

1 of 13

Yes

Atha\AT2G28620

1 of 13

Atha\AT2G30120

1 of 13

Yes

Atha\AT2G36200

1 of 13

Atha\AT2G37420

1 of 13

Atha\AT3G14750

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT5G02370

1 of 13

Atha\AT5G23910

1 of 13

Yes

Atha\AT5G27550

1 of 13

Atha\AT5G60930

1 of 13

Atha\AT5G61920

1 of 13

Yes

Atha\EMB2766

1 of 13

Atha\FRA1

1 of 13

Atha\ICR1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\KIP2

4 of 13

Yes

Scer\KIP3

2 of 13

Scer\KIP1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\tea2

3 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:cmet. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (27)

cana

11 of 13

Khc

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

nod

2 of 13

unc-104

2 of 13

CG5004

1 of 13

CG32318

1 of 13

cos

1 of 13

dpy

1 of 13

Klp10A

1 of 13

Klp59C

1 of 13

Klp59D

1 of 13

ncd

1 of 13

neb

1 of 13

Nup62

1 of 13

pav

1 of 13

sub

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

cancer, models of chromosomal instability

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

cmet^NIG.6392R

model of cancer

CEA with BacA\p35^UAS.cUa

(Clemente-Ruiz et al., 2014)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

CENPE; centromere protein E

model of primary autosomal recessive microcephaly 13

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CENPE; centromere protein E

10 of 14

117143

MICROCEPHALY 13, PRIMARY, AUTOSOMAL RECESSIVE; MCPH13

primary autosomal recessive microcephaly 13

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

cmet - Cen

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Fgop2

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Ndc80

anti tag coimmunoprecipitation, peptide massfingerprinting

(Przewloka et al., 2007)

cmet - Panx

experimental knowledge based

(Guruharsha et al., 2011)

cmet - RAF2

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Slmap

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Tlk

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Ubqn

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Zw10

anti bait coimmunoprecipitation, peptide massfingerprinting

(Williams et al., 2003)

cmet - Zwilch

anti bait coimmunoprecipitation, peptide massfingerprinting

(Williams et al., 2003)

cmet - dgt6

anti bait coimmunoprecipitation, western blot

(Bucciarelli et al., 2009)

cmet - dup

experimental knowledge based

(Guruharsha et al., 2011)

cmet - geminin

experimental knowledge based

(Guruharsha et al., 2011)

cmet - nxf2

experimental knowledge based

(Guruharsha et al., 2011)

cmet - row

experimental knowledge based

(Guruharsha et al., 2011)

cmet - woc

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cmet

enhanceable

Ndc80

(Radford et al., 2015)

cmet

suppressible

Spc105R

(Radford et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72792

DroID - A comprehensive database of gene and protein interactions.

FBgn0040232

MIST (protein-protein) - An integrated Molecular Interaction Database

53561

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-44

Cytogenetic map

32E2-32E2

Sequence location

2L:11,261,026..11,269,002 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

32E2-32E2

Limits computationally determined from genome sequence between P{PZ}l(2)04008⁰⁴⁰⁰⁸&P{PZ}cmet⁰⁴⁴³¹ and P{PZ}salm⁰³⁶⁰²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

32D-32E

(Yucel, 1999.12.30)

32E1-32E2

(determined by in situ hybridisation)

(Spradling et al., 1999, Perrimon et al., 1996)

32E1-32E2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-44

(FlyBase, 2016)

2-37.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

54004

y¹ v¹; P{TRiP.HMJ21427}attP40

11375

P{PZ}cmet⁰⁴⁴³¹ cn¹/CyO; ry⁵⁰⁶

32327

y¹ w^*; cmet^Δ/CyO, y⁺

19807

y¹ w^67c23; P{EPgy2}cmet^EY00718

35816

y¹ sc^* v¹ sev²¹; P{TRiP.GL00404}attP2

NIG-Fly

6392R-2

P{NIG.6392R}2

HMJ21427

y¹ v¹; P{TRiP.HMJ21427}attP40/CyO

M2L-0253

y¹ w¹¹¹⁸; cmet^SK1 P{neoFRT}40A/CyO-Tb

2LG-0204

y² VhaAC39-1^cho-1 v¹; P{U6-cmet.NIG.sgRNA}attP40/CyO

GL00404

y¹ sc^* v¹ sev²¹; P{TRiP.GL00404}attP2/TM3, Sb¹

VDRC

v330414

P{VSH330414}attP40

v35081

w¹¹¹⁸; P{GD11911}v35081

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (19)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040232

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040232

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Yucel et al., 2000)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cell morphology is aberrant, indicative of a failure in cell cycle/mitosis progression. This phenotype is not seen in Kc167 cells.

(Kiger et al., 2003)

cmet is required for the maintenance of chromosomes at the metaphase plate.

(Yucel et al., 2000)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. cmet gene expression during oogenesis is not critical to embryonic development, but the gene function may be essential for fertilisation and/or completion of meiosis.

(Perrimon et al., 1996)

Relationship to Other Genes

Source for database merge of

Source for merge of: cmet CG6392

(Greene, 2000.4.11, FlyBase, 1996-)

Source for merge of: cmet l(2)04431

(Misra, 2000.8.9)

Additional comments

Source for merge of cmet CG6392 was sequence comparison ( date:000725 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (22)

Reported As

Symbol Synonym

CENP-E

(Kondo et al., 2017, Clemente-Ruiz et al., 2014, Morais da Silva et al., 2013)

CENP-E meta

(Brunet and Vernos, 2001)

CENP-META

(Martins et al., 2009)

CENP-Meta

(Kwon et al., 2008)

CENP-meta

(Ye et al., 2015, Feijão et al., 2013, Moutinho-Pereira et al., 2013, Pool et al., 2012, Gerhold et al., 2011, Goshima et al., 2007, Maia et al., 2007, Maiato et al., 2006, Goshima and Vale, 2003)

CENPE

(Morais da Silva et al., 2013)

CENPmeta

(Przewloka et al., 2007)

CG6392

(National Institute of Genetics Fly Stocks, 2022-, Kondo et al., 2017, Meadows, 2017.9.14, Lee et al., 2013, Moutinho-Pereira et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Zhang et al., 2010, Hughes et al., 2008, Goshima et al., 2007, Przewloka et al., 2007, Molnar et al., 2006, Mount, 2001.8.14, Goldstein and Gunawardena, 2000, Greene, 2000.4.11)

Cenp-E

(Bucciarelli et al., 2009, Cenci et al., 2008, Musarò et al., 2008, Mentelova et al., 2007)

Cenp-M

(Dzhindzhev et al., 2005)

Cenp-meta

(Resende et al., 2018, Bucciarelli et al., 2009, Cenci et al., 2008)

Cenp_E

(Civelekoglu-Scholey et al., 2006)

Cmet

(Vicars et al., 2025, Khalili et al., 2023, Hirokawa, 2012.11.20, Miki et al., 2005, Miki et al., 2001)

DmCmet

(Hirokawa, 2012)

DmCmeta

(Dagenbach and Endow, 2004, Dagenbach and Endow, 2004, Kim and Endow, 2000)

KIF 10

(Miki et al., 2001)

KIF10

(Hirokawa, 2012.11.20)

l(2)04431

(Spradling et al., 1999)

Name Synonyms

CENP-meta

(Hirokawa, 2012.11.20, Gerhold et al., 2011, Przewloka et al., 2011, Fisher et al., 2008, Sawamura and Yamamoto, 2004, Yucel, 1999.12.30)

Cenp-meta

(Musarò et al., 2008, Dzhindzhev et al., 2005)

CenpMeta

(Karess, 2005)

Kinesin2A

(Kollmar, 2015.9.15)

cenp-meta

(Rubin et al., 2014, Wainman et al., 2012, Wainman et al., 2009, Williams et al., 2007)

Secondary FlyBase IDs

FBgn0010574
FBgn0032355

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53561

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VKI0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VKI0

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53561/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040232

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040232

FlyMine - An integrated database for Drosophila genomics

FBgn0040232

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6392

MARRVEL_MODEL - MARRVEL (model organism gene)

53561

Linkouts

BioGRID - A database of protein and genetic interactions.

72792

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040232

DroID - A comprehensive database of gene and protein interactions.

FBgn0040232

DRSC - Results frm RNAi screens

FBgn0040232

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

cmet_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040232

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040232

Flygut - An atlas of the Drosophila adult midgut

FBgn0040232

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0040232

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

MIST (protein-protein) - An integrated Molecular Interaction Database

53561

References (113)

The following external sites may use different assemblies or annotations than FlyBase.

0.37

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