Isolation and characterization of Df(2L)BSC241
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC241 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Nup154f06867 and P{XP}d07743. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}Nup154f06867/P{XP}d07743 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC241 from the segment of PBac{WH}Nup154f06867 to the left of its FRT site and the segment of P{XP}d07743 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC241 predicted from the Release 4 genomic coordinates of the transposable element insertions sites are 32C1;32F2. It failed to complement ab1, cmet04431 and salm1.