FlyBase Allele Report: Dmel\cmet[GD11911]

General Information

Symbol

Dmel\cmet^GD11911

Species

D. melanogaster

Name

FlyBase ID

FBal0209263

Feature type

allele

Associated gene

Dmel\cmet

Associated Insertion(s)

Carried in Construct

P{GD11911}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11911}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11911

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing cmet^GD11911 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 30-40% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11911

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

BacA\p35^UAS.cUa, Scer\GAL4^ap.PU, cmet^GD11911 has abnormal endomitotic cell cycle phenotype

(Clemente-Ruiz et al., 2014)

BacA\p35^UAS.cUa, Scer\GAL4^ap.PU, cmet^GD11911 has neoplasia phenotype

(Clemente-Ruiz et al., 2014)

BacA\p35^UAS.cUa, Scer\GAL4^en.PU, cmet^GD11911 has abnormal endomitotic cell cycle phenotype

(Clemente-Ruiz et al., 2014)

BacA\p35^UAS.cUa, Scer\GAL4^en.PU, cmet^GD11911 has neoplasia phenotype

(Clemente-Ruiz et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of cmet^GD11911 and BacA\p35^Scer\UAS.cUa in the dorsal compartment of the wing disc, under the control of Scer\GAL4^ap.PU, results in a high percentage of cells with DNA content higher than 4n (up to 30%) when compared to control cells. This is accompanied by some tissue overgrowth, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35^Scer\UAS.cUa-expressing control larvae and ultimately die.

Expression of cmet^GD11911 and BacA\p35^Scer\UAS.cUa in the posterior compartment of the wing disc, under the control of Scer\GAL4^en.PU, results in some tissue overgrowth. The resulting wing primordia are massively overgrown, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35^Scer\UAS.cUa-expressing control larvae and ultimately die.

Wing disc tissue expressing cmet^GD11911 and BacA\p35^Scer\UAS.cUa under the control of Scer\GAL4^ap.PU that has been transplanted into the abdomen of adult female flies (and maintained there for 12 days) grows several times larger than controls expressing BacA\p35^Scer\UAS.cUa alone and exhibits disorganised tissue architecture with extensive folding.

(Clemente-Ruiz et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v35081

w¹¹¹⁸; P{GD11911}v35081

Notes on Origin

Discoverer