Isolation and characterization of Df(2L)BSC242
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC242 was isolated as a FLP recombinase-induced recombination event involving P{XP}d07625 and PBac{WH}f02178.  The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d07625/PBac{WH}f02178 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females.  These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003).  This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002).  The recombination event generated the genetic element P+PBac{XP5.WH5}BSC242 from the segment of P{XP}d07625 to the left of its FRT site and the segment of PBac{WH}f02178 to the right of its FRT site.  Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC242 predicted from the Release 5 genomic coordinates of  the transposable element insertions sites are 32D4;32F4.  It failed to complement ab1, cmet04431 and salm1.