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Chougule, A., Lapraz, F., Földi, I., Cerezo, D., Mihály, J., Noselli, S. (2020). The Drosophila actin nucleator DAAM is essential for left-right asymmetry.  PLoS Genet. 16(4): e1008758.
FlyBase ID
FBrf0245622
Publication Type
Research paper
Abstract

Left-Right (LR) asymmetry is essential for organ positioning, shape and function. Myosin 1D (Myo1D) has emerged as an evolutionary conserved chirality determinant in both Drosophila and vertebrates. However, the molecular interplay between Myo1D and the actin cytoskeleton underlying symmetry breaking remains poorly understood. To address this question, we performed a dual genetic screen to identify new cytoskeletal factors involved in LR asymmetry. We identified the conserved actin nucleator DAAM as an essential factor required for both dextral and sinistral development. In the absence of DAAM, organs lose their LR asymmetry, while its overexpression enhances Myo1D-induced de novo LR asymmetry. These results show that DAAM is a limiting, LR-specific actin nucleator connecting up Myo1D with a dedicated F-actin network important for symmetry breaking.

PubMed ID
PubMed Central ID
PMC7200016 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS Genet.
    Title
    PLoS Genetics
    Publication Year
    2005-
    ISBN/ISSN
    1553-7404 1553-7390
    Data From Reference
    Alleles (73)
    Genes (38)
    Physical Interactions (1)
    Cell Lines (1)
    Natural transposons (1)
    Insertions (9)
    Experimental Tools (7)
    Transgenic Constructs (66)