FlyBase Gene Report: Dmel\Tbce

General Information

Symbol

Dmel\Tbce

Species

D. melanogaster

Name

Tubulin-binding cofactor E

Annotation Symbol

CG7861

Feature Type

protein_coding_gene

FlyBase ID

FBgn0033055

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

Tubulin-binding cofactor E (Tbce) encodes one of the five tubulin chaperones. It facilitates the folding of α-tubulin, a subunit of α-β tubulin heterodimers. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

Tubulin-specific chaperone E

Key Links

Genomic Location

Cytogenetic map

42A8-42A8

Sequence location

2R:6,042,532..6,044,964 [+]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:6042532..6044964

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables alpha-tubulin binding

inferred from biological aspect of ancestor with PANTHER:PTN001031924

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in microtubule polymerization

inferred from mutant phenotype

(Jin et al., 2009)

inferred from genetic interaction with FLYBASE:spas; FB:FBgn0039141

(Jin et al., 2009)

involved_in neuromuscular synaptic transmission

inferred from mutant phenotype

(Jin et al., 2009)

involved_in regulation of synaptic assembly at neuromuscular junction

inferred from mutant phenotype

(Jin et al., 2009)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in microtubule cytoskeleton organization

inferred from biological aspect of ancestor with PANTHER:PTN001031924

(GO Reference Genome Project, 2011-)

involved_in post-chaperonin tubulin folding pathway

inferred from biological aspect of ancestor with PANTHER:PTN001031924

(GO Reference Genome Project, 2011-)

involved_in tubulin complex assembly

inferred from biological aspect of ancestor with PANTHER:PTN001031924

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Jin et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001031924

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the TBCE family. (A1Z6J5)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Tubulin-binding cofactor E (Tbce) encodes one of the five tubulin chaperones. It facilitates the folding of α-tubulin, a subunit of α-β tubulin heterodimers. [Date last reviewed: 2019-03-14]

Protein Function (UniProtKB)

Tubulin-folding protein which is required for the development of the neuronal microtubule network. Essential for the development and function of neuromuscular synapses. Likely to promote microtubule formation by acting in the negative regulation of the microtubule-severing protein spas.

(UniProt, A1Z6J5)

Summary (Interactive Fly)

multidomain protein - required for development and function of neuromuscular synapses - promotes microtubule formation

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Tbce for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z6J5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Tbce-RB

FBtr0085974

NM_206025

2080

523

Tbce-RC

FBtr0335286

NM_136353

2144

523

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Tbce-PB

FBpp0085327

59.4

523

6.58

FBpp0307274

59.4

523

6.58

A1Z6J5

NP_610197

AAF57288

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

523 aa isoforms: Tbce-PB, Tbce-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

A1Z6J5

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tbce using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.93

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Jin et al., 2009)

larval ventral nerve cord

(Jin et al., 2009)

larval muscle system

(Jin et al., 2009)

larval stage

embryonic/larval epidermis

(Jin et al., 2009)

third instar larval stage

larval central nervous system

(Jin et al., 2009)

larval muscle system

Comment: perinuclear enrichment seen

(Jin et al., 2009)

adult stage | male

spermatid axoneme

(Nuwal et al., 2012)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis

(Nuwal et al., 2012)

Additional Descriptive Data

Tbce staining marks the axoneme bundles in the spermatogenic cyst apical to the F-actin-rich individualization complex.

(Nuwal et al., 2012)

Tbce is ubiquitously expressed in embryos with particular enrichment in the neuromusculature. In third instar larvae, weak expression is observed in muscles, central neurons, and peripheral axons and strong expression is seen in the epidermis.

(Jin et al., 2009)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Jin et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Tbce in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Tbce

View exonic expression by tissue for Dmel\Tbce

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033055

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35532/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033055

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033055

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033055

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7861

Flygut - An atlas of the Drosophila adult midgut

FBgn0033055

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Tbce

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 9-10
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 19 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Tbce

Transgenic constructs containing regulatory region of Tbce

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)Exel7085

(Ryder, 2004.4.26)

Df(2R)tbce^LH15

(Jin et al., 2009)

Gene partially disrupted in

Df(2R)tbce^LH15

(Jin et al., 2009)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

Tbce^GD10779

lethal - all die before end of pupal stage, with Scer\GAL4^elav-C155

Tbce^RNAi.UAS.cJa

lethal - all die during embryonic stage (with Tbce^LH15)

Tbce^Z0241

lethal - all die during embryonic stage (with Tbce^Z0241)

Tbce^LH15

partially lethal, with Scer\GAL4^tin.CΔ4

Tbce^GD10779

viable, with Scer\GAL4^C57

Tbce^RNAi.UAS.cJa

Other Phenotypes

Allele

abnormal locomotor behavior | larval stage, with Scer\GAL4^C57

abnormal locomotor behavior | larval stage, with Scer\GAL4^elav-C155

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^69B

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^69B, Tbce^GD10779

Tbce^KK100119

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^69B, Tbce^KK100119

Tbce^GD10779

abnormal neuroanatomy | cell autonomous | somatic clone, with Scer\GAL4^GH146

Tbce^RNAi.UAS.cJa

abnormal neuroanatomy | embryonic stage

abnormal neuroanatomy | larval stage, with Scer\GAL4^C57

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

Tbce^RNAi.UAS.cJa

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Tbce^GD10779

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^69B, Tbce^GD10779

Tbce^KK100119

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^69B, Tbce^KK100119

Tbce^GD10779

decreased size | third instar larval stage, with Scer\GAL4^69B, Tbce^GD10779

Tbce^KK100119

decreased size | third instar larval stage, with Scer\GAL4^69B, Tbce^KK100119

Tbce^GD10779

some die during embryonic stage (with Tbce^LH15)

Tbce^Z0241

some die during embryonic stage (with Tbce^Z0241)

Tbce^LH15

some die during pupal stage, with Scer\GAL4^elav-C155

Tbce^RNAi.UAS.cJa

Phenotype manifest in

Allele

adult antennal lobe projection neuron DL1 adPN | cell autonomous | somatic clone, with Scer\GAL4^GH146

Tbce^RNAi.UAS.cJa

chromosome | third instar larval stage, with Scer\GAL4^69B, Tbce^GD10779

Tbce^KK100119

chromosome | third instar larval stage, with Scer\GAL4^69B, Tbce^KK100119

Tbce^GD10779

embryonic/larval neuromuscular junction, with Scer\GAL4^C57

embryonic/larval neuromuscular junction, with Scer\GAL4^elav-C155

Tbce^RNAi.UAS.cJa

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^RapGAP1-OK6

Tbce^UAS.cJa

embryonic neuroblast | third instar larval stage, with Scer\GAL4^69B

embryonic neuroblast | third instar larval stage, with Scer\GAL4^69B, Tbce^GD10779

Tbce^KK100119

embryonic neuroblast | third instar larval stage, with Scer\GAL4^69B, Tbce^KK100119

Tbce^GD10779

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Tbce^GD10779

larval brain | third instar larval stage, with Scer\GAL4^69B

larval brain | third instar larval stage, with Scer\GAL4^69B, Tbce^GD10779

Tbce^KK100119

larval brain | third instar larval stage, with Scer\GAL4^69B, Tbce^KK100119

Tbce^GD10779

larval longitudinal connective

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

Tbce^GD10779

larval ventral nerve cord

microtubule, with Scer\GAL4^C57

microtubule (with Df(2R)ED1484)

Tbce^Z0241

microtubule & neuromuscular junction, with Scer\GAL4^elav-C155

mitotic spindle | third instar larval stage, with Scer\GAL4^69B

mitotic spindle | third instar larval stage, with Scer\GAL4^69B, Tbce^GD10779

Tbce^KK100119

mitotic spindle | third instar larval stage, with Scer\GAL4^69B, Tbce^KK100119

Tbce^GD10779

mitotic spindle microtubule | third instar larval stage, with Scer\GAL4^69B, Tbce^GD10779

Tbce^KK100119

mitotic spindle microtubule | third instar larval stage, with Scer\GAL4^69B, Tbce^KK100119

Tbce^GD10779

NMJ bouton, with Scer\GAL4^C57

NMJ bouton, with Scer\GAL4^elav-C155

Tbce^RNAi.UAS.cJa

type Ib terminal bouton | increased number | larval stage, with Scer\GAL4^RapGAP1-OK6

Tbce^UAS.cJa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (25)

Hsap\TBCE

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (23)

Rnor\Tbce

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Nisch

1 of 14

Rnor\Podnl1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (21)

Mmus\Tbce

13 of 14

Yes

Mmus\Aspn

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (22)

Xtro\tbce

11 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\lrrc23

1 of 13

Xtro\lrriq1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (18)

Drer\tbce

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\tbce-1

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (23)

Agam\AgaP_AGAP010770

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP006348

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (11)

Atha\PFI

12 of 13

Yes

Atha\AT4G36180

1 of 13

Yes

Atha\AT4G39270

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\RLP26

1 of 13

Atha\RLP30

1 of 13

Atha\RLP35

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\PAC2

12 of 13

Yes

Scer\CYR1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\alp21

7 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Tbce. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (14)

mlt

3 of 13

2mit

1 of 13

atk

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Lrt

1 of 13

Sur-8

1 of 13

trn

1 of 13

U2A

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

hypoparathyroidism-retardation-dysmorphism syndrome

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 8 )

Allele

Disease

Evidence

References

Tbce^GD10779

model of hypoparathyroidism-retardation-dysmorphism syndrome

CEA

(Métivier et al., 2021)

CEA with Tbce^KK100119

(Métivier et al., 2021)

Tbce^KK100119

model of hypoparathyroidism-retardation-dysmorphism syndrome

CEA

(Métivier et al., 2021)

CEA with Tbce^GD10779

(Métivier et al., 2021)

Tbce^Z0241

model of hypoparathyroidism-retardation-dysmorphism syndrome

CEA with Tbce^LH15

(Métivier et al., 2021)

Tbce^LH15

model of hypoparathyroidism-retardation-dysmorphism syndrome

CEA with Tbce^Z0241

(Métivier et al., 2021)

Tbce^RNAi.UAS.cJa

model of hypoparathyroidism-retardation-dysmorphism syndrome

CEA

(Jin et al., 2009)

Tbce^{mutNLS.UASp.EGFP}

model of hypoparathyroidism-retardation-dysmorphism syndrome

CEA

(Métivier et al., 2021)

Tbce^{mutCG.UASp.EGFP}

model of hypoparathyroidism-retardation-dysmorphism syndrome

CEA

(Métivier et al., 2021)

Tbce^{HRD.UASp.EGFP}

DOES NOT model hypoparathyroidism-retardation-dysmorphism syndrome

CEA

(Métivier et al., 2021)

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

TBCE; tubulin folding cofactor E

model of hypoparathyroidism-retardation-dysmorphism syndrome

IEA

(FlyBase, 2019-)

model of Kenny-Caffey syndrome type 1

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TBCE; tubulin folding cofactor E

14 of 14

604934

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Tbce - Arl2

anti tag coimmunoprecipitation, anti tag western blot

(Chen et al., 2016)

Tbce - Pen

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry

(Métivier et al., 2021)

Tbce - Ran

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry

(Métivier et al., 2021)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Tbce

enhanceable

mad2

(Métivier et al., 2021)

Tbce

suppressible

mad2

(Métivier et al., 2021)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Tbcd

enhanceable

Tbce

(Okumura et al., 2015)

αTub84B

suppressible

Tbce

(Saunders et al., 2022)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0033055

MIST (genetic) - An integrated Molecular Interaction Database

35532

MIST (protein-protein) - An integrated Molecular Interaction Database

35532

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42A8-42A8

Sequence location

2R:6,042,532..6,044,964 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42A8-42A8

Limits computationally determined from genome sequence between P{PZ}l(2)09851⁰⁹⁸⁵¹&P{lacW}Src42A^k10108 and P{lacW}l(2)k09848^k09848&P{EP}EP407

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-55.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

18286

w¹¹¹⁸; PBac{WH}Tbce^f00024/CyO

34536

w¹¹¹⁸; P{UAS-tbce.J}3/TM6B, Tb¹

34535

w^*; Tbce^LH15/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

34537

w^*; wg^Sp-1/CyO; P{UAS-tbce.RNAi.J}3/TM6B, Tb¹

44569

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02865}attP40

FlyORF

F001553

M{UAS-tbce.ORF}ZH-86Fb

F001258

M{UAS-Tbce.ORF.3xHA}ZH-86Fb

NIG-Fly

7861R-1

P{NIG.7861R}1

2RG-0894

y² VhaAC39-1^cho-1 v¹; P{U6-Tbce.NIG.sgRNA}attP40/CyO

HMS02865

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02865}attP40/CyO

VDRC

v34388

P{GD10779}v34388

v105246

P{KK100119}VIE-260B

More stocks available...

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (78)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GM13256

Other clones

FI05242

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0033055

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0033055

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Métivier et al., 2021)

monoclonal

(Jin et al., 2009)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

New stable cell line derived from S2R+ : S2R+ cell expressing wildtype or mutant Tbce from the Act5C promoter were created.
(Métivier et al., 2021)

Other Comments

One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

(Inlow and Restifo, 2004)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: tbce CG7861

(Jin et al., 2009)

Source for identity of: Tbce tbce

(FlyBase, 2009-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Name Synonyms

Tubulin-binding cofactor E

Tubulin-specific chaperone E

(Jin et al., 2009)

tubulin-specific chaperone E

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35532

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z6J5

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A1Z6J5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z6J5

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0033055

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35532/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033055

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033055

FlyMine - An integrated database for Drosophila genomics

FBgn0033055

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7861

MARRVEL_MODEL - MARRVEL (model organism gene)

35532

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0033055

DroID - A comprehensive database of gene and protein interactions.

FBgn0033055

DRSC - Results frm RNAi screens

FBgn0033055

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033055

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0033055

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7861

Flygut - An atlas of the Drosophila adult midgut

FBgn0033055

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC030612

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

35532

MIST (protein-protein) - An integrated Molecular Interaction Database

35532

References (49)

The following external sites may use different assemblies or annotations than FlyBase.

1.93

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