FlyBase Allele Report: Dmel\Tbce[RNAi.UAS.cJa]

General Information

Symbol

Dmel\Tbce^RNAi.UAS.cJa

Species

D. melanogaster

Name

FlyBase ID

FBal0241482

Feature type

allele

Associated gene

Dmel\Tbce

Associated Insertion(s)

Carried in Construct

P{UAS-tbce.RNAi.J}

Key Links

Transgenic product class

RNAi_reagent

(Jin et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Jin et al., 2009)

Mutagen

in vitro construct

(Jin et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-tbce.RNAi.J}

Cytology

Description

UAS regulatory sequences drive expression of a hairpin of Tbce sequences, which consists of a cDNA fragment from the second exon fused to the corresponding genomic sequence plus intron 2 as a spacer (this targets nucleotides 337-842 of GenBank sequence NM_136353).

(Jin et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tbce

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of hypoparathyroidism-retardation-dysmorphism syndrome

CEA

(Jin et al., 2009)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN | cell autonomous | somatic clone, with Scer\GAL4^GH146

(Okumura et al., 2015)

embryonic/larval neuromuscular junction, with Scer\GAL4^C57

(Jin et al., 2009)

embryonic/larval neuromuscular junction, with Scer\GAL4^elav-C155

(Jin et al., 2009)

microtubule, with Scer\GAL4^C57

(Jin et al., 2009)

microtubule & neuromuscular junction, with Scer\GAL4^elav-C155

(Jin et al., 2009)

NMJ bouton, with Scer\GAL4^C57

(Jin et al., 2009)

NMJ bouton, with Scer\GAL4^elav-C155

(Jin et al., 2009)

Detailed Description

Statement

Reference

DL1 olfactory projection neuron clones expressing Tbce^{dsRNA.Scer\UAS.cJa} under the control of Scer\GAL4^GH146 exhibit ectopic dendrite arborization, but axon morphology is unaffected.

(Okumura et al., 2015)

Larvae expressing tbce^{dsRNA.Scer\UAS.cJa} under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^C57 show normal rhythmic peristalsis and crawling activity. However, the larvae perform less well in a roll-over assay than control flies, having a significantly increased roll-over time.

Expression of tbce^{dsRNA.Scer\UAS.cJa} under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^C57 results in an increase in synaptic branching and bouton number and a decrease in average bouton area at the third larval instar neuromuscular junction (NMJ) compared to controls.

Expression of tbce^{dsRNA.Scer\UAS.cJa} under the control of Scer\GAL4^elav-C155 results in an increase in the amplitude of excitatory junction potentials (EJPs) and in the amplitude and frequency of miniature EJPs (mEJPs) at the NMJ compared to controls. Quantal content is normal.

Expression of tbce^{dsRNA.Scer\UAS.cJa} under the control of Scer\GAL4^C57 has no effect on the amplitude of excitatory junction potentials (EJPs) or the amplitude or frequency of miniature EJPs (mEJPs) at the NMJ compared to controls. Quantal content is normal.

Expression of tbce^{dsRNA.Scer\UAS.cJa} under the control of Scer\GAL4^elav-C155 results in decreased, interrupted or missing microtubules at the distal part of the NMJ synaptic terminal.

Expression of tbce^{dsRNA.Scer\UAS.cJa} under the control of Scer\GAL4^C57 decreases the microtubule network in muscles, with sparser and shorter microtubule fibres.

(Jin et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Tbce^RNAi.UAS.cJa/Scer\GAL4^GH146 is an enhancer of abnormal neuroanatomy | cell autonomous | somatic clone phenotype of Tbcd¹

(Okumura et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Tbce^RNAi.UAS.cJa/Scer\GAL4^GH146 is an enhancer of adult antennal lobe projection neuron DL1 adPN | cell autonomous | somatic clone phenotype of Tbcd¹

(Okumura et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tbce^{dsRNA.Scer\UAS.cJa} under the control of Scer\GAL4^GH146 enhances the frequency of dendrite defects seen in TBCD¹ mutant DL1 projection neuron clones.

(Okumura et al., 2015)

When tbce^{dsRNA.Scer\UAS.cJa} and spas^Scer\UAS.cTa are co-expressed under the control of Scer\GAL4^C57, the microtubule phenotype in the muscles is similar to that of animals expressing spas^Scer\UAS.cTa alone under the control of Scer\GAL4^C57.

(Jin et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34537

w^*; wg^Sp-1/CyO; P{UAS-tbce.RNAi.J}3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Tbce^RNAi.UAS.cJa

Tbce^{dsRNA.Scer\UAS.cJa}

Tbce^{dsRNA.UAS.cJa}

tbce^{dsRNA.Scer\UAS.cJa}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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